排序方式: 共有32条查询结果,搜索用时 15 毫秒
11.
Juan C Rondan Due?as Cristina N Gardenal Guillermo Albrieu Llinás Graciela M Panzetta-Dutari 《Génome》2006,49(8):931-937
The complete A+T - rich region of Aedes aegypti mitochondrial DNA has been cloned and sequenced. In Argentinean populations of the species, a polymorphism in the length of the amplified fragment was observed. Nucleotide sequence comparison of the shortest and longest A+T - rich amplified fragments detected revealed the presence of 2 types of tandemly repeated blocks. The size variation observed in natural populations is mainly due to the presence of a variable number of a 181 bp tandem repeat unit, located toward the 12S rRNA gene end. The size of the longest A+T - rich region was of 2070 bp, representing the largest control sequence reported for any mosquito species. Few relevant short blocks of primary-sequence similarity conserved in the control region of mosquitoes and other insects were detected scattered throughout the whole region. Five putative stem-loop secondary structures were found, one of them flanked by conserved sequences described in other insects. Our results suggest that there are no universal models of structure-function relations in the control region of insect mtDNA. In addition, we identified a short A+T - rich variable segment in the Ae. aegyti control region that would be suitable for population genetic studies. 相似文献
12.
Danillo Gardenal Augusto Bruno Zagonel Piovezan Luiza Tamie Tsuneto Sidia Maria Callegari-Jacques Maria Luiza Petzl-Erler 《PloS one》2013,8(2)
Although the KIR gene content polymorphism has been studied worldwide, only a few isolated or Amerindian populations have been analyzed. This extremely diverse gene family codifies receptors that are expressed mainly in NK cells and bind HLA class I molecules. KIR-HLA combinations have been associated to several diseases and population studies are important to comprehend their evolution and their role in immunity. Here we analyzed, by PCR-SSP (specific sequencing priming), 327 individuals from four isolated groups of two of the most important Brazilian Amerindian populations: Kaingang and Guarani. The pattern of KIR diversity among these and other ten Amerindian populations disclosed a wide range of variation for both KIR haplotypes and gene frequencies, indicating that demographic factors, such as bottleneck and founder effects, were the most important evolutionary factors in shaping the KIR polymorphism in these populations. 相似文献
13.
The Greater Rhea (Rhea americana) is a characteristic bird of the Argentine Pampas. Despite the increasing farming interest of this ratite, their natural populations are progressively decreasing in size and range. The object of this study was to evaluate the status of captive populations as potential genetic reservoirs. Using Inter‐Simple Sequence Repeats as molecular markers, levels of genetic variability of F1 individuals from two captive populations were estimated and compared with those of wild populations in the same region. The captive populations were polymorphic for 12.22 and 13.33% of the loci, with a genetic diversity of 0.050. Differences with wild populations were not significant (z=1.79; P>0.05). Therefore, captive populations of rheas in Argentina should not be overlooked as genetic reservoir and source of individuals for reinforcement of natural populations, through reintroduction and translocation. Zoo Biol 30:65–70, 2011. © 2010 Wiley‐Liss, Inc. 相似文献
14.
González-Ittig RE Salazar-Bravo J Polop JJ Gardenal CN 《Molecular ecology resources》2008,8(6):1466-1468
The rodent Oligoryzomys longicaudatus or long-tailed pygmy rice rat is the reservoir of the aetiological agent of the hantavirus pulmonary syndrome in southern Argentina and Chile. We characterize 11 polymorphic microsatellite loci which would be useful for studies on microgeographical population structure in the species. Amplification of these loci in 42 individuals from four natural populations revealed four to 21 alleles per locus, and values of observed heterozygosities ranging from 0.371 to 0.896. Cross-species amplifications showed that some of the primers designed may be useful for other species of the genus Oligoryzomys. 相似文献
15.
Gladys B. de Sousa Adolfo Jiménez Antonio Blanco Cristina N. Gardenal 《Biochemical genetics》1997,35(11-12):339-349
Aedes albifasciatus is a floodwater mosquito widely distributed in Argentina. It is important from economic and medical points of view. A 4-year survey of seasonal variation in allele frequencies in a population of this species was undertaken to determine possible changes in the genetic structure and their correlation with environmental conditions. Significant temporal variation was detected at most of the loci, but it did not follow a cyclic or seasonal pattern. Multivariate analysis of principal components showed a remarkable homogeneity of samples collected from December 1993 to April 1995 and a clear differentiation of the November 1991, March 1992, and November 1993 samples. This variation could be correlated with the magnitude of rainfall occurring in the area. Passive transport of larvae by water streams and river freshets produced by floods would have mixed larvae from breeding sites with different allele frequencies, causing the genetic differentiation observed. 相似文献
16.
Phylogenetic relationships among species of the Neotropical genus Graomys (Rodentia: Cricetidae): contrasting patterns of skull morphometric variation and genetic divergence
下载免费PDF全文
![点击此处可从《Biological journal of the Linnean Society. Linnean Society of London》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Juan J. Martínez Cristina N. Gardenal 《Biological journal of the Linnean Society. Linnean Society of London》2016,118(3):648-667
Subtle differences of external traits characterize species of rodents in the Neotropical genus Graomys. On the other hand, the species differ markedly in chromosome number. In the present study, we evaluate the possible evolutionary forces involved in the evolution of the genus by assessing the degree of intra‐ and interspecific genetic and morphological variation. A phylogenetic analysis demonstrates the existence of at least three species with high levels of genetic distance (10%), which diverged between 1 and 1.5 Mya. Neither Graomys griseoflavus, nor Graomys chacoensis present marked phylogeographical structure. Regarding morphological characters, these species show shape differences in the skull that could be attributable to differences in the local conditions they inhabit, being more marked in G. griseoflavus than in G. chacoensis. The skull shape of G. chacoensis could have evolved under genetic drift, whereas evidence reported in the present study indicates that this character could be under selective pressures in G. griseoflavus. Reconstruction of the ancestral area suggests that G. griseoflavus originated in the central Monte desert, whereas G. chacoensis originated in the Chaco ecoregion surrounding the austral extreme of the Yungas rainforest. Subsequently, both species would have undergone demographic and geographical expansions almost simultaneously, starting approximately 150 000–175 000 years ago. The complex evolutionary history of the genus could be partly explained by the decoupling of morphological, karyological and molecular traits. 相似文献
17.
18.
Juan J. Martínez Raúl E. González‐Ittig Gerardo R. Theiler Ricardo Ojeda Cecilia Lanzone Agustina Ojeda Cristina N. Gardenal 《Journal of Zoological Systematics and Evolutionary Research》2010,48(2):159-166
To increase our understanding of the speciation process occurred in the sibling species Graomys griseoflavus and Graomys centralis, a phylogeographic study was conducted based on sequences of a hypervariable segment of the mtDNA D‐loop region. The resulting haplotype phylogenetic network showed two well‐defined clusters, one for each species. The clusters were connected by two haplotypes from localities that are almost 300 km apart, one situated in the Monte eco‐region and the other, in the Chaco. This result is in agreement with a previous hypothesis about the geographical context in which the cladogenetic process occurred. A divergence time of 0.15–0.28 million years was estimated, which is consistent with a process of recent speciation. An amova test confirmed that at present gene flow between species does not exist. The mismatch distribution analyses suggest that the geographical and demographic expansion undergone by the species is related to the climatic events that occurred in the region during the Quaternary. 相似文献
19.
Sassi PL Chiappero MB Borghi C Gardenal CN 《Journal of experimental zoology. Part A, Ecological genetics and physiology》2011,315(6):337-348
The small cavy Microcavia australis, a social and fossorial rodent, inhabits a large distribution range in South American arid zones. The species is versatile in coping with the seasonal and spatial variability typical of these environments through changes in morphology, physiology, and behavior. In order to explore whether phenotypic variations are related to the evolutionary history of the species, we analyzed the levels of genetic variability and divergence among four populations that differ in climate and habitat characteristics, two belonging to highlands and the other two from lowlands. We sequenced the mitochondrial control region and used the Inter Simple Sequence Repeats technique to study variability in the noncoding nuclear genome. Results from both genetic markers were consistent. Variability levels were high for all populations, and even higher for lowland ones. Pairwise genetic differentiation varied greatly, all comparisons being statistically significant except for the two highland populations. Seventeen haplotypes were detected which displayed three clear lineages: two corresponding to each lowland population and one to those in the highlands. Levels of genetic differentiation between population pairs varied widely. Haplotypes showed a mean sequence divergence of 1.4% between lowland populations and 0.2% between highland ones, whereas divergence was around 9% when populations from different altitudes were compared. Results from BEAST analysis support extant hypotheses suggesting that lowland forms are clearly older than the highland group. The deep genetic divergence between lineages poses the need to search for new evidence for properly defining the taxonomic status of divergent populations of M. australis. 相似文献
20.
Calomys musculinus is a Sigmodontinae rodent inhabiting periodically disturbed habitats in the central eastern plains of Argentina. It is the natural reservoir host of Junin virus, the etiological agent of Argentine Hemorrhagic Fever (AHF). In order to analyze the levels of gene flow among populations of this species, allozymic variability at 26 loci was studied in 291 individuals from the endemic zone of AHF and localities outside it. All populations showed high levels of polymorphism (H
e between 0.107 and 0.144; P95% between 38 and 54%). Individual loci f values were in most cases negative, although not significantly different from zero. Mean genetic differentiation among populations was low, but statistically significant (=0.020; P<0.01). There was no correlation between genetic and geographic distances between pairs of populations, and scatter of the pairwise points suggests that, at the regional scale, genetic drift is more influential than gene flow. This result can be interpreted as indicative of a relatively recent expansion of C. musculinus habitat and restricted on-going gene flow, which would be compatible with a relatively slow expansion rate of AHF. 相似文献