Design,synthesis and biological evaluation of novel desloratadine derivatives with anti-inflammatory and H1 antagonize activities

To improve the anti-inflammatory activity of desloratadine, we designed and synthesized a series of novel desloratadine derivatives. All compounds were evaluated for their anti-inflammatory and H₁ antagonistic activities. Among them, compound 2c showed the strongest H₁ antagonistic and anti-inflammatory activity. It also exhibited promising pharmacokinetic profiles and low toxicity. All these results suggest that compound 2c as a novel anti-allergic agent is worthy of further investigation.     

西北干旱区葡萄净光合速率变化及其影响因素

葡萄作为西北干旱区主要经济作物之一,认识其光合生产过程对种植栽培至关重要。为探究大田自然条件下葡萄光合生理特征及影响葡萄光合作用的主要影响因子,该研究于2019年6—9月测定葡萄(品种：无核白)叶片光合作用及其生理生态因子日变化,采用通径分析方法分析各因子对叶片净光合速率的直接和间接作用,确定其主要影响因子,同时在全天分时段模式下进一步分析葡萄叶片净光合速率对各生理生态因子的响应。结果表明：(1)葡萄叶片净光合速率日变化总体呈现先升高、后下降的单峰型曲线变化特征。(2)葡萄叶片净光合速率与光合有效辐射、饱和水汽压差、空气温度、气孔导度和蒸腾速率呈极显著正相关,与相对湿度和胞间CO₂浓度呈极显著负相关。(3)各月影响葡萄叶片净光合速率变化的主要决定因子6月、8月和9月为蒸腾速率,而7月为气孔导度。(4)6—9月的葡萄叶片净光合速率与空气温度、光合有效辐射、饱和水汽压差的响应均呈“迟滞回环”关系,与蒸腾速率、气孔导度呈良好的线性关系(R²>0.85),与胞间CO₂浓度呈指数函数关系(R²=0.53...     

The Role of Kv1.2 Channel in Electrotaxis Cell Migration

Voltage‐gated potassium Kv1.2 channels play pivotal role in maintaining of resting membrane potential and, consequently, regulation of cellular excitability of neurons. Endogenously generated electric field (EF) have been proven as an important regulator for cell migration and tissue repair. The mechanisms of ion channel involvement in EF‐induced cell responses are extensively studied but largely are poorly understood. In this study we generated three COS‐7 clones with different expression levels of Kv1.2 channel, and confirmed their functional variations with patch clamp analysis. Time‐lapse imaging analysis showed that EF‐induced cell migration response was Kv1.2 channel expression level depended. Inhibition of Kv1.2 channels with charybdotoxin (ChTX) constrained the sensitivity of COS‐7 cells to EF stimulation more than their motility. Immunocytochemistry and pull‐down analyses demonstrated association of Kv1.2 channels with actin‐binding protein cortactin and its re‐localization to the cathode‐facing membrane at EF stimulation, which confirms the mechanism of EF‐induced directional migration. This study displays that Kv1.2 channels represent an important physiological link in EF‐induced cell migration. The described mechanism suggests a potential application of EF which may improve therapeutic performance in curing injuries of neuronal and/or cardiac tissue repair, post operational therapy, and various degenerative syndromes. J. Cell. Physiol. 231: 1375–1384, 2016. © 2015 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc.     

酸解法制取甘草次酸及其纯化工艺

以甘草酸单铵盐为原料,酸解制取甘草次酸并利用低温冷析法对其进行精制纯化.通过L9(34)正交试验,确定了酸解甘草次酸的最佳条件:H2SO4体积分数8％、料液比(g/mL)1:100、温度100℃、12 h,在此条件下,转化率可达80.24％:通过对比实验,获得了低温冷析法精制甘草次酸的纯化工艺:酸解产物经水洗、氯仿溶提后,在体积分数30％乙醇溶液中80℃热溶15 min,再在冰水中冷析10 min,最终得到甘草次酸的质量分数为95.79％,得率40.87％.     

1H, 15N and 13C resonance assignments for the three LOTUS RNA binding domains of Tudor domain-containing protein TDRD7

The LOTUS or OST-HTH domain is a recently discovered motif of about 80 amino acids and is found in several germline-specific proteins including the Tudor domain-containing proteins TDRD5 and TDRD7, which are important for germ cell development. The LOTUS domain is an RNA binding domain but its exact function is unknown. Here, we report the ¹H, ¹³C and ¹⁵N resonance assignments for the three LOTUS domains present in mouse TDRD7. These assignments will allow three-dimensional structure determination of the LOTUS domains and mapping of their interaction with RNA, steps toward deciphering the function of TDRD7.     

Bacillus subtilis核糖体蛋白质S13和BL18突变体的遗传分析

以诱变分离得到Bacillus subtilis W168核糖体蛋白质S3、S7、S8、S11、S13、BL2、BL6、BL7突变体。通过遗传分析定位了S13和BL18基因。S13基因(rps M)位于主要核糖体蛋白质基因群str A-spc A区中,而BL18基因(rpl S)和pyr D紧密连锁。BL 18突变对细胞RNA和蛋白质合成以及噬菌体φ29的成斑率没有明显影响。     

薇甘菊不同生长方式下的繁殖特征比较

利用实验生态学方法, 对攀缘生长与伏地生长的薇甘菊(Mikania micrantha)的形态特征、生物量分配和繁殖特征进行了比较研究。结果表明: 在潮湿生境条件下, 攀缘生长的植株与伏地生长的植株相比对主茎的生物量投资较大, 而对分枝茎的生物量投资则相对较小; 但在干旱生境条件下, 攀缘生长的植株对主茎和分枝茎的生物量投资均较伏地生长的植株小。在潮湿与干旱生境中, 攀缘植株的繁殖投资分别为0.429 g·g^–1和0.342 g·g^–1, 显著高于相同生境中的伏地植株。在薇甘菊种群的补充与更新过程中, 攀缘生长的植株以有性生殖占主导地位, 在潮湿与干旱生境中产生的个体分别占种群数量的84.7%和62.6%; 伏地生长的植株则主要以无性(克隆)繁殖为主, 在潮湿与干旱生境中有性生殖产生的个体仅占种群数量的40.4%和35.9%。生长方式与土壤水分二因子互作效应对薇甘菊的生物量分配和繁殖指标影响均达到显著水平(P<0.05)。通过研究得出以下结论: 不同生长方式的薇甘菊对环境条件具有不同的生长与繁殖适应对策。     

内生驱动因素对景感意愿度差异的影响——从城市公园斑块-廊道-基质景观角度

城市绿色空间与人的健康息息相关,新发展阶段人们对城市绿色空间建设提出了更高要求,来满足日益增长的美好生活需要。优化城市绿色空间,维持、提升和增加城市生态系统服务,是景感生态学研究的使命之一,然而以往研究鲜有从人自身的人格属性角度探讨内生驱动因素在景观感知和景观评价中的影响。以景感意愿度作为衡量景观感知和需求的指标,探究内生驱动因素对人的景观感知和行为偏好选择的影响,提出内生驱动因素对人在不同景观类型中的感受存在差异,而且这种差异影响到个体的行为偏好这一假设。以杭州市主城区5座城市公园的"斑块-廊道-基质"景观作为研究对象,以MBTI量表所得的人格类型作为内生驱动因素,将内生驱动因素与景观和行为偏好选择进行有序回归分析,探讨其对景感意愿度的影响。结果表明：基质景观中受访者景感意愿度最强烈,其次为斑块景观,廊道景观中景感意愿度最低;在斑块景观中,ESFP （外向-感官-感性-自然）型、ENFP （外向-畅想-感性-自然）型、ESFJ （外向-感官-感性-控制）型和INFP （内向-畅想-感性-自然）型的人格类型对景感意愿度具有显著相关关系;在廊道景观中,有14种人格类型对景感意愿度具有显著相关关系;在基质景观中,有9种人格类型对景感意愿度具有显著相关关系。研究结果证实了内生驱动因素影响着人们的景感意愿度。在城市景观生态评价、规划和景感营造,以及生态系统服务研究中,不仅需要考虑生态环境因素和人的社会属性,还应考虑到人的内在属性特征,将景感意愿度和内生驱动因素纳入生态规划中。     

北京地区设施草莓8种病毒的酶联免疫吸附测定检测

[背景]病毒可以随同草莓无性繁殖材料传播扩散,导致产量和品质下降.选育无病毒种苗是草莓病毒病防治的主要措施,高效、灵敏的检测技术可为草莓病毒病防治提供技术保障.[目的]为明确8种能够侵染草莓的病毒在北京地区设施草莓上的发生情况,应用酶联免疫吸附测定(Enzyme-Linked Immunosorbent Assay,E...