全文获取类型
收费全文 | 16640篇 |
免费 | 1248篇 |
国内免费 | 1245篇 |
专业分类
19133篇 |
出版年
2024年 | 42篇 |
2023年 | 242篇 |
2022年 | 579篇 |
2021年 | 952篇 |
2020年 | 578篇 |
2019年 | 769篇 |
2018年 | 761篇 |
2017年 | 559篇 |
2016年 | 788篇 |
2015年 | 1047篇 |
2014年 | 1295篇 |
2013年 | 1423篇 |
2012年 | 1520篇 |
2011年 | 1372篇 |
2010年 | 832篇 |
2009年 | 744篇 |
2008年 | 853篇 |
2007年 | 712篇 |
2006年 | 573篇 |
2005年 | 511篇 |
2004年 | 420篇 |
2003年 | 371篇 |
2002年 | 270篇 |
2001年 | 250篇 |
2000年 | 223篇 |
1999年 | 233篇 |
1998年 | 160篇 |
1997年 | 135篇 |
1996年 | 122篇 |
1995年 | 110篇 |
1994年 | 103篇 |
1993年 | 91篇 |
1992年 | 103篇 |
1991年 | 102篇 |
1990年 | 53篇 |
1989年 | 55篇 |
1988年 | 43篇 |
1987年 | 33篇 |
1986年 | 22篇 |
1985年 | 27篇 |
1984年 | 23篇 |
1983年 | 15篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 10 毫秒
141.
142.
Aolin Jia Yan Ren Fengmei Gao Guihong Yin Jindong Liu Lu Guo Jizhou Zheng Zhonghu He Xianchun Xia 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(5):1063-1071
Key message
Four QTLs for adult-plant resistance to powdery mildew were mapped in the Zhou8425B/Chinese Spring population, and a new QTL on chromosome 3B was validated in 103 wheat cultivars derived from Zhou8425B.Abstract
Zhou8425B is an elite wheat (Triticum aestivum L.) line widely used as a parent in Chinese wheat breeding programs. Identification of genes for adult-plant resistance (APR) to powdery mildew in Zhou8425B is of high importance for continued controlling the disease. In the current study, the high-density Illumina iSelect 90K single-nucleotide polymorphism (SNP) array was used to map quantitative trait loci (QTL) for APR to powdery mildew in 244 recombinant inbred lines derived from the cross Zhou8425B/Chinese Spring. Inclusive composite interval mapping identified QTL on chromosomes 1B, 3B, 4B, and 7D, designated as QPm.caas-1BL.1, QPm.caas-3BS, QPm.caas-4BL.2, and QPm.caas-7DS, respectively. Resistance alleles at the QPm.caas-1BL.1, QPm.caas-3BS, and QPm.caas-4BL.2 loci were contributed by Zhou8425B, whereas that at QPm.caas-7DS was from Chinese Spring. QPm.caas-3BS, likely to be a new APR gene for powdery mildew resistance, was detected in all four environments. One SNP marker closely linked to QPm.caas-3BS was transferred into a semi-thermal asymmetric reverse PCR (STARP) marker and tested on 103 commercial wheat cultivars derived from Zhou8425B. Cultivars with the resistance allele at the QPm.caas-3BS locus had averaged maximum disease severity reduced by 5.3%. This STARP marker can be used for marker-assisted selection in improvement of the level of powdery mildew resistance in wheat breeding.143.
Li Sun Ming Xiu Shuhua Wang David R. Brigstock Hongyan Li Limei Qu Runping Gao 《Journal of cellular and molecular medicine》2018,22(4):2346-2356
Pancreatic stellate cells (PSCs) play a critical role in fibrogenesis during alcoholic chronic pancreatitis (ACP). Transforming growth factor‐beta1 (TGF‐β1) is a key regulator of extracellular matrix production and PSC activation. Endotoxin lipopolysaccharide (LPS) has been recognized as a trigger factor in the pathogenesis of ACP. This study aimed to investigate the mechanisms by which LPS modulates TGF‐β1 signalling and pancreatic fibrosis. Sprague‐Dawley rats fed with a Lieber‐DeCarli alcohol (ALC) liquid diet for 10 weeks with or without LPS challenge during the last 3 weeks. In vitro studies were performed using rat macrophages (Mφs) and PSCs (RP‐2 cell line). The results showed that repeated LPS challenge resulted in significantly more collagen production and PSC activation compared to rats fed with ALC alone. LPS administration caused overexpression of pancreatic TLR4 or TGF‐β1 which was paralleled by an increased number of TLR4‐positive or TGF‐β1‐positive Mφs or PSCs in ALC‐fed rats. In vitro, TLR4 or TGF‐β1 production in Mφs or RP‐2 cells was up‐regulated by LPS. LPS alone or in combination with TGF‐β1 significantly increased type I collagen and α‐SMA production and Smad2 and 3 phosphorylation in serum‐starved RP‐2 cells. TGF‐β pseudoreceptor BAMBI production was repressed by LPS, which was antagonized by Si‐TLR4 RNA or by inhibitors of MyD88/NF‐kB. Additionally, knockdown of Bambi with Si‐Bambi RNA significantly increased TGF‐β1 signalling in RP‐2 cells. These findings indicate that LPS increases TGF‐β1 production through paracrine and autocrine mechanisms and that LPS enhances TGF‐β1 signalling in PSCs by repressing BAMBI via TLR4/MyD88/NF‐kB activation. 相似文献
144.
145.
Chiakang Ho Ya Gao Danning Zheng Yanjun Liu Shengzhou Shan Bin Fang Yixuan Zhao Dingzhong Song Yifan Zhang Qingfeng Li 《Journal of cellular and molecular medicine》2019,23(8):5108-5118
Obesity and its associated metabolic disorders such as diabetes, hepatic steatosis and chronic heart diseases are affecting billions of individuals. However there is no satisfactory drug to treat such diseases. In this study, we found that alisol A, a major active triterpene isolated from the Chinese traditional medicine Rhizoma Alismatis, could significantly attenuate high‐fat‐diet‐induced obesity. Our biochemical detection demonstrated that alisol A remarkably decreased lipid levels, alleviated glucose metabolism disorders and insulin resistance in high‐fat‐diet‐induced obese mice. We also found that alisol A reduced hepatic steatosis and improved liver function in the obese mice model.In addition, protein expression investigation revealed that alisol A had an active effect on AMPK/ACC/SREBP‐1c pathway. As suggested by the molecular docking study, such bioactivity of alisol A may result from its selective binding to the catalytic region of AMPK.Therefore, we believe that Alisol A could serve as a promising agent for treatment of obesity and its related metabolic diseases. 相似文献
146.
Bo Song Jürg Stcklin Deli Peng Yongqian Gao Hang Sun 《Botanical journal of the Linnean Society. Linnean Society of London》2015,179(2):349-359
Environmental conditions are known to affect the reproductive fitness of insects, as do host plants. Although the highly specialized bracts of Himalayan ‘glasshouse’ plants are thought to be an adaptive morphology that allows the plant to reproduce in harsh alpine environments, little information appears to exist concerning the benefit of these bracts for the pollinating insects. In this study, we examine whether the semi‐translucent bracts of Rheum alexandrae, a giant herb endemic to the alpine zone of the Hengduan Mountains in south‐western China, enhance the reproductive fitness of its pollinator. Rheum alexandrae depends mainly on mutualistic seed‐consuming Bradysia flies for pollination. Bracts increased interior temperature on sunny days, acted as a buffer against fluctuating air humidity, greatly decreased the intensities of ultraviolet B/C radiation and provided shelter from strong winds. Bract removal significantly decreased adult oviposition and offspring performance of pollinators during flowering and fruiting, respectively. Our results indicate that the bracts of R. alexandrae enhance the reproductive fitness of its pollinating seed‐consuming mutualist in alpine environments, which may be attributed to the positive effects of the bracts on the interior microenvironment. © 2015 The Linnean Society of London, Botanical Journal of the Linnean Society, 2015, 179 , 349–359. 相似文献
147.
Successful immune-mediated regression of solid tumors is difficult because of the small number of cytotoxic T lymphocytes (CTLs) that were traffic to the tumor site. Here, the targeting of tumor-specific infiltrating CTLs was dependent on a fusion protein consisting of human epidermal growth factor (EGF) and staphylococcal enterotoxin A (SEA) with the D227A mutation. EGF-SEA strongly restrained the growth of murine solid sarcoma 180 (S180) tumors (control versus EGF-SEA, mean tumor weight: 1.013 versus 0.197 g, difference = 0.816 g). In mice treated with EGF-SEA, CD4+, CD8+ and SEA-reactive T lymphocytes were enriched around the EGFR expressing tumor cells. The EGF receptors were potentially phosphorylated by EGF-SEA stimulation and the fusion protein promoted T cells to release the tumoricidal cytokines interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α). Intratumoral CTLs secreted cytolytic pore-forming perforins and granzyme B proteins near the surface of carcinomas, causing the death of many tumor cells. We additionally show that labeled EGF-SEA was directly targeted to the tumor tissue after intravenous (i.v.) injection. The findings demonstrate that antibody-like EGF-SEA plays an important role in arresting CTLs in the solid tumor site and has therapeutic potential as a tumor-targeting agent. 相似文献
148.
Bo Wang Deliang Shen Junnan Tang Jing Li Yue Xiao Xiuying Chen Chang Cao Dongjian Han Erhe Gao Wen Zhao Jinying Zhang Junbiao Chang 《Journal of cellular and molecular medicine》2019,23(9):6048-6059
Sodium (±)‐5‐bromo‐2‐(a‐hydroxypentyl) benzoate (generic name: brozopine, BZP) has been reported to protect against stroke‐induced brain injury and was approved for Phase II clinical trials for treatment of stroke‐related brain damage by the China Food and Drug Administration (CFDA). However, the role of BZP in cardiac diseases, especially in pressure overload‐induced cardiac hypertrophy and heart failure, remains to be investigated. In the present study, angiotensin II stimulation and transverse aortic constriction were employed to induce cardiomyocyte hypertrophy in vitro and in vivo, respectively, prior to the assessment of myocardial cell autophagy. We observed that BZP administration ameliorated cardiomyocyte hypertrophy and excessive autophagic activity. Further results indicated that AMP‐activated protein kinase (AMPK)‐mediated activation of the mammalian target of rapamycin (mTOR) pathway likely played a role in regulation of autophagy by BZP after Ang II stimulation. The activation of AMPK with metformin reversed the BZP‐induced suppression of autophagy. Finally, for the first time, we demonstrated that BZP could protect the heart from pressure overload‐induced hypertrophy and dysfunction, and this effect is associated with its inhibition of maladaptive cardiomyocyte autophagy through the AMPK‐mTOR signalling pathway. These findings indicated that BZP may serve as a promising compound for treatment of pressure overload‐induced cardiac remodelling and heart failure. 相似文献
149.
Synergistic interactions between Glomus mosseae and Bradyrhizobium japonicum in enhancing proton release from nodules and hyphae 总被引:1,自引:0,他引:1
Soybean (Glycine max L. Merr.) seedlings were inoculated with Glomus mosseae (GM) and Bradyrhizobium japonicum (BJ) together or separately to study the effect of interactions on net H+ effluxes of nodules or extraradical hyphae by in vivo vibrating electrode techniques. GM promoted three-fold the H+ effluxes of nodules on mycorrhizal lateral roots and BJ increased eight-fold the net H+ effluxes of hyphae developing in the vicinity of nodules on lateral roots. Increments in plant P content were positively
and linearly correlated with the net H+ efflux of nodules and hyphae. It is concluded that increased H+ effluxes of nodules resulted from enhanced nitrogenase activities induced by the presence of the AM fungus in lateral roots.
The results point to additive effects of interactions between mycorrhizal fungi and rhizobia in increasing the extent of acidification
of the “nodulesphere” and the hyposphere. 相似文献
150.