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Gao Kai Liu Meiyou Li Yuan Wang Lei Zhao Chao Zhao Xian Zhao Jinyi Ding Yi Tang Haifeng Jia Yanyan Wang Jingwen Wen Aidong 《Journal of molecular histology》2021,52(3):449-459
Journal of Molecular Histology - Currently, the excessive activation of N-methyl-D-aspartate receptors (NMDARs) is considered to be a crucial mechanism of brain injury. Lycium barbarum A (LyA) is a... 相似文献
165.
Plasmonics - Tunable local surface plasmon resonance (LSPR) enhancement properties of cavity-nanoparticle scaffold-based clusters were investigated via finite-difference time-domain (FDTD)... 相似文献
166.
Biology Bulletin - CRISPR is an adaptive immune defense system found in bacteria and archaea that is resistant to heterologous invasive genetic material. Later studies showed that the CRISPR system... 相似文献
167.
Gao X. Zhang Y. Mu J. Q. Chen K. X. Zhang H. F. Bi K. S. 《Russian Journal of Bioorganic Chemistry》2021,47(2):420-431
Russian Journal of Bioorganic Chemistry - Guan-Xin-Shu-Tong capsule is a widely used traditional Chinese medicine for the treatment of cardiovascular diseases. However, little knowledge about the... 相似文献
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X.C. Xu X. Abuduhadeer W.B. Zhang T. Li H. Gao Y.H. Wang 《European journal of histochemistry : EJH》2013,57(4)
The receptor for advanced glycation end-products (RAGE) is an oncogenic trans-membranous receptor, which is overexpressed in multiple human cancers. However, the role of RAGE in gastric cancer is still elusive. In this study, we investigated the expression and molecular mechanisms of RAGE in gastric cancer cells. Forty cases of gastric cancer and corresponding adjacent non-cancerous tissues (ANCT) were collected, and the expression of RAGE was assessed using immunohistochemistry (IHC) in biopsy samples. Furthermore, RAGE signaling was blocked by constructed recombinant small hairpin RNA lentiviral vector (Lv-shRAGE) used to transfect into human gastric cancer SGC-7901 cells. The expression of AKT, proliferating cell nuclear antigen (PCNA) and matrix metallopeptidase-2 (MMP-2) was detected by Real-time PCR and Western blot assays. Cell proliferative activities and invasive capability were respectively determined by MTT and Transwell assays. Cell apoptosis and cycle distribution were analyzed by flow cytometry. As a consequence, RAGE was found highly expressed in cancer tissues compared with the ANCT (70.0% vs 45.0%, P=0.039), and correlated with lymph node metastases (P=0.026). Knockdown of RAGE reduced cell proliferation and invasion of gastric cancer with decreased expression of AKT, PCNA and MMP-2, and induced cell apoptosis and cycle arrest. Altogether, upregulation of RAGE expression is associated with lymph node metastases of gastric cancer, and blockade of RAGE signaling suppresses growth and invasion of gastric cancer cells through AKT pathway, suggesting that RAGE may represent a potential therapeutic target for this aggressive malignancy.Key words: RAGE, gastric cancer, growth, invasion 相似文献
170.
Cuiqin Li Laping He Baoquan Qiu Bing Gao 《Preparative biochemistry & biotechnology》2013,43(4):354-365
Novozyme 435 could be a highly efficient catalyst in the asymmetric acylation of (R,S)-3-n-butylphthalide in tetrahydrofuran–hexane solvents. The effect of various reaction parameters such as agitation velocity, water content, mixed media, temperature, concentration of Novozyme 435, molar ratio of acetic anhydride to (R,S)-3-n-butylphthalide, reaction time, enantiomeric excess of substrate (eeS), enantiomeric excess of product (eeP), and enantioselective ratio (E) were studied. Tetrahydrofuran markedly improved (R,S)-3-n-butylphthalide conversion, enantiomeric excess of remaining 3-n-butylphthalide, and enantiomeric ratio. The optimum media were 50% (v/v) tetrahydrofuran and 50% (v/v) hexane. Other ideal reaction conditions were an agitation velocity of 150 rpm, 0.4% (v/v) water content, temperature of 30°C, 8 mg/mL dosage of Novozyme 435, 8:1 (0.4 mmol: 0.05 mmol) molar ratio of acetic anhydride to (R,S)-3-n-butylphthalide, and a reaction time of 48 hr. Under the optimum conditions, 96.4% eeS and 49.3% conversion of (R,S)-3-n-butylphthalide were achieved. In addition, enantiomeric excess of the product was above 98.0%. 相似文献