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91.
Crude and immunoaffinity-purified excretory-secretory antigens derived from a domestic pig isolate of Trichinella spiralis were used in an enzyme-linked immunosorbent assay to test serum from mice infected with 25 different pig and wild animal isolates of T. spiralis sspp. All of the sera were found positive by ELISA using either of the antigen preparations, indicating all isolates shared certain antigen epitopes. Excretory-secretory antigens were prepared from 3 distinct isolates of T. spiralis sspp.--Trichinella spiralis spiralis (pig isolate), Trichinella spiralis nativa (polar bear isolate), and Trichinella spiralis pseudospiralis--and compared by electrophoresis and monoclonal antibody binding. While protein profiles varied among the isolates, a monoclonal antibody recognizing a major immunodiagnostic antigen epitope bound all 3 antigen preparations. However, this antigen epitope occurred on different molecular weight excretory-secretory proteins from the different isolates. 相似文献
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93.
J L Gamble 《Biochimica et biophysica acta》1973,323(2):240-250
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95.
Evidence for the absence of photoreduction of the metal centers of cytochrome C oxidase by X-irradiation 总被引:1,自引:0,他引:1
Samples of X-irradiated cytochrome c oxidase were examined by electron paramagnetic resonance and optical spectroscopy. Both radiation from the Stanford Synchrotron Radiation Laboratory and a conventional X-ray source (W target) were utilized. The X-ray flux from these sources ranges from 10(9) to 10(13) photons/s. No evidence was found for photoreduction of the metal centers in the enzyme by X-ray photons. These results demonstrate that the integrity of cytochrome c oxidase is maintained using the conditions under which X-ray absorption measurements are presently being made. 相似文献
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98.
MacDonald JI Dietrich A Gamble S Hryciw T Grant RI Meakin SO 《Journal of neurochemistry》2012,121(6):861-880
Vesicular transport in neurons plays a vital role in neuronal function and survival. Nesca is a novel protein that we previously identified and herein describe its pattern of expression, subcellular localization and protein-protein interactions both in vitro and in vivo. Specifically, a large proportion of Nesca is in tight association with both actin and microtubule cytoskeletal proteins. Nesca binds to F-actin, microtubules, βIII and acetylated α-tubulin, but not neurofilaments or the actin-binding protein drebrin, in in vitro-binding assays. Nesca co-immunoprecipitates with kinesin heavy chain (KIF5B) and kinesin light-chain motors as well as with the synaptic membrane precursor protein, syntaxin-1, and is a constituent of the post-synaptic density. Moreover, in vitro-binding assays indicate that Nesca directly binds KIF5B, kinesin light-chain and syntaxin-1. In contrast, Nesca does not co-immunoprecipitate with the kinesin motors KIF1B, KIF3A nor does it bind syntaxin-4 or the synaptosome-associated protein 25 kDa (SNAP-25) in vitro. Nesca expression in neurons is highly punctuate, co-stains with syntaxin-1, and is found in fractions containing markers of early endosomes and Golgi suggesting that it is involved in vesicular transport. Collectively, these data suggest that Nesca functions as an adapter involved in neuronal vesicular transport including vesicles containing soluble N-ethylmaleimide sensitive factor attachment protein receptors that are essential to exocytosis. 相似文献
99.
Vidya Limaye Pu Xia Chris Hahn Malcolm Smith Mathew A. Vadas Stuart M. Pitson Jennifer R. Gamble 《Cellular & molecular biology letters》2009,14(3):424-441
Sphingosine kinase-1 (SK1) promotes the formation of sphingosine-1-phosphate (S1P), which has potent pro-inflammatory and
pro-angiogenic effects. We investigated the effects of raised SK1 levels on endothelial cell function and the possibility
that this signaling pathway is activated in rheumatoid arthritis. Human umbilical vein endothelial cells with 3- to 5-fold
SK1 (ECSK) overexpression were generated by adenoviral and retroviralmediated gene delivery. The activation state of these cells and
their ability to undergo angiogenesis was determined. S1P was measured in synovial fluid from patients with RA and OA. ECSK showed an enhanced migratory capacity and a stimulated rate of capillary tube formation. The cells showed constitutive activation
as evidenced by the induction of basal VCAM-1 expression, and further showed a more augmented VCAM-1 and E selectin response
to TNF compared with empty vector control cells (ECEV). These changes had functional consequences in terms of enhanced neutrophil binding in the basal and TNFstimulated states
in ECSK. By contrast, over-expression of a dominant-negative SK inhibited the TNF-induced VCAM-1 and E selectin and inhibited PMN
adhesion, confirming that the observed effects were specifically mediated by SK. The synovial fluid levels of S1P were significantly
higher in patients with RA than in those with OA. Small chronic increases in SK1 activity in the endothelial cells enhance
the ability of the cells to support inflammation and undergo angiogenesis, and sensitize the cells to inflammatory cytokines.
The SK1 signaling pathway is activated in RA, suggesting that manipulation of SK1 activity in diseases of aberrant inflammation
and angiogenesis may be beneficial. 相似文献
100.
Megan D. Gamble Christopher E. Bagwell Jeannine LaRocque Peter W. Bergholz Charles R. Lovell 《Microbial ecology》2010,59(2):253-265
Nitrogen fixation is the primary N source in the highly productive but N-limited North Inlet, SC, USA salt marsh system. The
diverse assemblages of nitrogen-fixing (diazotrophic) bacteria associated with the rhizospheres of the short and tall growth
forms of Spartina alterniflora were analyzed at two sites, Crab Haul Creek and Goat Island, which are in different tidal creek drainage systems in this
marsh. The sites differed in proximity to the main channel for tidal intrusion and in several edaphic parameters. We hypothesized
that either the differing abiotic environmental regimes of the two sites or the variation due to seasonal effects result in
differences in the diazotroph assemblage. Rhizosphere samples were collected seasonally during 1999 and 2000. DNA was purified
and nifH amplified for denaturing gradient gel electrophoresis (DGGE) analysis of diazotroph assemblage composition. Principal components
analysis was used to analyze the binary DGGE band position data. Season strongly influenced assemblage composition and biplots
were used to identify bands that significantly affected the seasonal and site-specific clustering. The types of organisms
that were most responsive to seasonal or site variability were identified on the basis of DGGE band sequences. Seasonally
responsive members of the anaerobic diazotrophs were detected during the winter and postsenescence conditions and may have
been responsible for elevated pore water sulfide concentrations. Sequences from a diverse assemblage of Gammaproteobacteria
were predominant during growth periods of S. alterniflora. Abiotic environmental parameters strongly influenced both the S. alterniflora and the diazotrophic bacterial assemblages associated with this keystone salt marsh plant species. 相似文献