排序方式: 共有64条查询结果,搜索用时 15 毫秒
41.
A E Gurvich N A Galstian D A El'gort 《Biulleten' eksperimental'no? biologii i meditsiny》1986,102(10):501-504
New microcarriers for the growth of animal cells have been synthesized and studied. The preparations are porous cellulose beads, modified by diamines. Spreading and growth of L cells, MEVO and HETR cells on these beads were observed. As a result of the cultivation the number of animal cells increased 5-10-fold. 相似文献
42.
The procedures for isolation and purification of Cu/Zn superoxide dismutase (SOD) from small amounts of rat liver at different stages of Pliss lymphosarcoma growth were developed. Two stages of tumour growth were distinguished. At the first stage (4-5th day after reinoculation) the intensity of optical (680 nm) and EPR spectra of SOD was decreased, on the average, to 30%, while at the second stage--by 40% at 680 nm and by 32%, at 260 nm. The intensity of the EPR spectra was also diminished by 40% as compared to the control. It was assumed that at initial stages of tumour growth the decrease of the SOD activity is mainly due to the reduction of enzyme Cu, while at the second stage--to the decrease of the protein content. In all probability, the observed changes are induced by the activation of lipid peroxidation involving active O2 species. The second stage is associated with the superoxide-induced impairment of the function of nucleic acids involved in protein biosynthesis. 相似文献
43.
Kunakbaeva AF Karazhas NV Zigangirova NA Rybalkina TN Galstian GM Osmanov EA Petrosov VV Bosh'ian RE Savitskaia NV Feklisova LV Iuditskiĭ MV 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2006,(7):100-103
Results of study of rooms' air and washes from medical equipment by PCR assay to detect Pneumocystis carinii DNA are presented. PCR assay sensivity was 200 copies/ml. Method of taking of air samples by MC-2 sample-taking device was modified for P. carinii detection. Sensivity of the method was 10 copies/m3. 27 air samples and 105 washes from medical equipment were studied and P. carinii DNA was not detected. It has been shown during the study that DNA of pneumocysts remains intact at room temperature during 12 days including 2-hour ultraviolet (UV) radiation treatment. After processing of studied surfaces with 0.1% solution of chloramine with subsequent UV radiation treatment during 30 minutes results of PCR assay were negative. 相似文献
44.
45.
46.
47.
48.
49.
50.