全文获取类型
收费全文 | 1723篇 |
免费 | 159篇 |
出版年
2022年 | 6篇 |
2021年 | 26篇 |
2020年 | 13篇 |
2019年 | 14篇 |
2018年 | 16篇 |
2017年 | 20篇 |
2016年 | 44篇 |
2015年 | 62篇 |
2014年 | 69篇 |
2013年 | 78篇 |
2012年 | 109篇 |
2011年 | 116篇 |
2010年 | 80篇 |
2009年 | 59篇 |
2008年 | 104篇 |
2007年 | 106篇 |
2006年 | 82篇 |
2005年 | 91篇 |
2004年 | 96篇 |
2003年 | 94篇 |
2002年 | 97篇 |
2001年 | 15篇 |
2000年 | 17篇 |
1999年 | 24篇 |
1998年 | 27篇 |
1997年 | 28篇 |
1996年 | 18篇 |
1995年 | 25篇 |
1994年 | 19篇 |
1993年 | 24篇 |
1992年 | 25篇 |
1991年 | 22篇 |
1990年 | 17篇 |
1989年 | 17篇 |
1988年 | 7篇 |
1987年 | 11篇 |
1986年 | 19篇 |
1985年 | 14篇 |
1984年 | 15篇 |
1983年 | 15篇 |
1982年 | 21篇 |
1981年 | 15篇 |
1980年 | 22篇 |
1979年 | 11篇 |
1977年 | 10篇 |
1976年 | 6篇 |
1975年 | 13篇 |
1973年 | 7篇 |
1972年 | 6篇 |
1971年 | 7篇 |
排序方式: 共有1882条查询结果,搜索用时 31 毫秒
81.
The Drosophila dec-1 gene produces three proproteins required for female fertility and eggshell assembly. The three proproteins are distinguished by their C termini. Fc106, the most abundant proprotein, is cleaved within the vitelline membrane to three mature derivatives in a developmentally regulated manner. To define sequences within fc106 that are critical for its function, we created wild-type and mutant versions of an fc106 cDNA transgene. The functional consequences of the mutations were assessed in dec-14, a female-sterile splicing mutant that does not produce the fc106 isoform. The fertility of dec-14 females was restored by the introduction of either a wild-type transgene or a transgene bearing a C-terminal deletion that included fc106-specific sequences. Surprisingly, the removal of internal coding sequences created an aberrant DEC-1 proprotein that induced female sterility when introduced into wild-type flies. Dominant female sterility was not associated with larger deletions that included the fc106 N terminus, suggesting that abnormal juxtaposition of N- and C-terminal sequences in the aberrant proprotein interfered with endogenous DEC-1 proteins. Changes in the fractionation behavior of the endogenous fc106 C-terminal derivative, s60, and morphological changes in the endochorion in response to expression of the aberrant proprotein support this interpretation. 相似文献
82.
83.
Preston GM 《Cell host & microbe》2007,2(5):291-294
Type III secretion systems (T3SS) are used by bacteria to deliver proteins into the cytoplasm of eukaryotic cells. Most studies of T3SS have focused on the role of these secretion systems in specific host interactions. However, there is increasing evidence to suggest that T3SS are also used in interactions with multiple hosts, thereby promoting opportunistic infections and mutualistic symbioses. 相似文献
84.
85.
86.
87.
88.
A new cotton variant with reduced levels of terpenoid aldehydes (sesquiterpenoids and sesterterpenoids (heliocides)) was isolated from the progeny of hemizygous cotton (Gossypium hirsutum cv. Coker 312) transformed with antisense (+)-delta-cadinene synthase cDNA. Southern analysis of leaf DNA digested with HindIII, Pst or KpnI restriction endonucleases did not detect any antisense cdn1-C1 DNA in the genome of the variant. The gossypol content in the seed of the variant was markedly lower than in the seed of T1 antisense plants. Eighty-nine percent of the variant seed had a 71.1% reduction in gossypol and the foliage of the variant plants showed a 70% reduction in gossypol and a 31% reduction in heliocides. Compared to non-transformed plants there was no reduction in the number of lysigenous glands in the seed of the variant. The cotton variant shows uncoupling of terpenoid aldehyde synthesis and gland formation. The cotton variant may have resulted from somaclonal variation occurring in the callus tissue during the transformation-regeneration process. 相似文献
89.
Lieberman DE Krovitz GE Yates FW Devlin M St Claire M 《Journal of human evolution》2004,46(6):655-677
Changes in the technology of food preparation over the last few thousand years (especially cooking, softening, and grinding) are hypothesized to have contributed to smaller facial size in humans because of less growth in response to strains generated by chewing softer, more processed food. While there is considerable comparative evidence to support this idea, most experimental tests of this hypothesis have been on non-human primates or other very prognathic mammals (rodents, swine) raised on hard versus very soft (nearly liquid) diets. Here, we examine facial growth and in vivo strains generated in response to raw/dried foods versus cooked foods in a retrognathic mammal, the rock hyrax (Procavia capensis). The results indicate that the hyrax cranium resembles the non-human primate cranium in having a steep gradient of strains from the occlusal to orbital regions, but differs from most non-anthropoids in being primarily twisted; the hyrax mandible is bent both vertically and laterally. In general, higher strains, as much as two-fold at some sites, are generated by masticating raw versus cooked food. Hyraxes raised on cooked food had significantly less growth (approximately 10%) in the ventral (inferior) and posterior portions of the face, where strains are highest, resembling many of the differences evident between humans raised on highly processed versus less processed diets. The results support the hypothesis that food processing techniques have led to decreased facial growth in the mandibular and maxillary arches in recent human populations. 相似文献
90.
Since the nineteenth century, researchers have noted that Neandertal and modern human adults differ in mental foramen position, although the ontogenetic changes in the position of this feature have only recently come under the scrutiny of paleoanthropologists. Research on mental foramen position has focused on whether this feature is inferior to a particular tooth. However, tooth position may not be a reliable indicator of mental foramen position because of variability in tooth size within and between taxa and during eruption events. As opposed to observing the mental foramen with respect to the postcanine teeth, we examined linear distances from the mental foramen to other mandibular landmarks. Modern human adults may appear truncated, or paedomorphic, in mental foramen position with respect to Neandertal adults. However, infants of the two taxa differ substantially in anterior mandibular form. The initial differences in the shape of the mental region may be related to the embryological position of the mental foramen in modern humans and its role in the development of the mental trigone. The shape changes that accrue thereafter, possibly from faster mandibular growth rates in Neandertals, further distinguish the adults from one another. Although mandibular shape differences exist from early infancy onwards, adults of the two taxa are broadly similar in bi-mental foramen breadth with respect to mandibular size. For this reason, qualitative assessments of mental foramen position may provide less taxonomic information than previously thought. 相似文献