水稻花药总蛋白质双向电泳方法的改良与优化(简报)

双向电泳作为蛋白质组学核心技术之一,目前已广泛地应用在植物领域,并且成功应用于水稻代谢和调节等方面的研究。谢锦云等利用溶液法提取温敏核不育水稻花药总蛋白质,利用pH3-10线性胶条分离,经银染显色后检测到约1,000个     

Virus-like particles in Eimeria tenella are associated with multiple RNA segments

Total nucleic acids from sporulated oocysts of Eimeria tenella isolated from Changchun in China were found to contain three extrachromosomal double-stranded RNA segments (dsRNAs) of 1.4, 2.4 and 3.6 kb in sizes. These RNAs were resistant to RNase A digestion under high salt concentration (0.3 M NaCl). RNA-dependent RNA polymerase (RDRP) activity was detected in crude extracts of E. tenella sporulated oocysts containing these nucleic acid species. Virus-like particles (VLPs) were shown to have a diameter of approximately 38 nm under Electron Microscopy (EM) after purification by sucrose density gradient centrifugation. In keeping with the nomenclature generally adopted for protozoan viruses, we have named this isolate as E. tenella virus (ETV) which is the first virus isolated from E. tenella.     

赤霉素对总状绿绒蒿三个居群种子萌发特性的影响

采集生长于滇西北不同海拔梯度的3个总状绿绒蒿居群种子,分别采用不同浓度的赤霉素(GA3)处理后,进行种子萌发实验。结果表明:(1)种子的长宽比和千粒重随海拔升高而增加,差异显著(P<0.05);种子含水量随海拔升高而降低,且差异显著(P<0.05)。(2)不同居群的对照种子发芽率随海拔升高而增加,平均发芽时间随海拔升高而缩短。(3)100~250 mg·L-1的GA3处理可提高种子的发芽率和发芽势,且差异极显著(P<0.001),但GA3处理对平均发芽时间无显著影响(P>0.05)。同时,各居群萌发指标的最适GA3处理浓度不一致。(4)居群对总状绿绒蒿种子的发芽率和发芽势无显著影响(P>0.05),而对种子平均发芽时间影响显著(P<0.05)。说明海拔是造成不同居群种子外部形态和种子萌发特性差异的原因之一,而不同浓度的GA3对各居群种子的萌发有不同程度的影响。     

PROTEIN S-ACYL TRANSFERASE 13/16 modulate disease resistance by S-acylation of the nucleotide binding,leucine-rich repeat protein R5L1 in Arabidopsis

Nucleotide binding, leucine-rich repeat(NB-LRR)proteins are critical for disease resistance in plants,while we do not know whether S-acylation of these proteins plays a role during bacterial infection. We identified 30 Arabidopsis mutants with mutations in NB-LRR encoding genes from the Nottingham Arabidopsis Stock Center and characterized their contribution to the plant immune response after inoculation with Pseudomonas syringae pv tomato DC3000(Pst DC3000). Of the five mutants that were hyper-...     

水稻红莲型细胞质雄性不育系小孢子不同发育时期花药总蛋白质比较分析

采用双向凝胶电泳对水稻红莲型细胞质雄性不育的不育系小孢子发育单核期和二核期花药总蛋白进行了分离,通过银染显色,获得了分辨率和重复性较好的双向电泳图谱,且单核期和二核期花药总蛋白质在双向电泳胶上分布的图谱十分相似。PDQuest 2DE图像分析软件在等电点(pI)3.0~10.0、分子量(M.W.)9.0~98.0 kD之间可识别约1 800个蛋白质点。比较分析发现单核期和二核期花药中共有241个差异表达的蛋白质点,其中仅在单核期中表达的点数为125,仅在二核期中表达的为13点;表现为表达量差异的105点,其中在二核期表达下调的点数为70点,表达上调的为33点。还对蛋白质点集中的区域(pI 4.5~8.0,M.W.25.0~70.0 kD)中的41个差异蛋白质点进行了分子量和等电点分析。     

Cytochrome oxidase from thermophilic bacterium PS3 contains a fourth protein subunit

Monoclonal antibodies prepared against subunits II and IV of beef heart cytochrome oxidase were found to cross-react with thermophilic bacterial PS3 oxidase. Each individual antibody affects the enzymatic activity. "Western" blot analyses showed that subunit II antibodies of beef heart recognized subunit II of PS3 and subunit IV antibody likewise recognized a fourth protein subunit on slab gels. This fourth subunit previously thought to be a contaminant or a degradation product has a molecular weight of about 10,500 on SDS-gels, and appears to exist in stoichiometric amount. We have extracted this subunit from slab gels and compared its amino acid composition with that of subunit III.     

美人蕉矮化病植株中发现的一种类似类病毒的RNA

北京栽培的多年生植物美人蕉(Canna Indica)发生一种矮化病(CaSD),病叶组织中未发现病毒颗粒,但发现一种分子量约为1.9×10~5的核酸。这种核酸对核糖核酸酶敏感,抗脱氧核糖核酸酶,称谓CaSD-RNA。在CF-11纤维素柱上层析,90％以上CaSD-RNA可在含15％乙醇缓冲液中洗脱下来。分子展层后用暗场电镜观察,在不变性条件下为类似棒状的结构。在变性条件下为环状分子结构。用双向聚丙烯酰胺凝胶电泳技术证实,CaSD-RNA为环状分子。其侵染性尚待证实。     

Determining beta-sheet stability by Fourier transform infrared difference spectra

We describe here a new method for determining the conformational stability of antiparallel beta-sheets. Due to coupling between the transition dipoles, beta-sheet conformations typically exhibit a characteristic high-frequency amide I component centered at approximately 1680 cm(-1). Using one beta-sheet protein and two small beta-hairpins, we demonstrate that this high-frequency component, which is fairly narrow (approximately 8-10 cm(-1)), can be quantitatively resolved and used in thermal stability determination. Compared with the commonly used CD and fluorescence techniques, this ir method offers advantages. Since the area of this high-frequency component is only proportional to the folded population, it eliminates the need for a priori information of the folded and unfolded baselines encountered in other methods. Thus, it is applicable to a variety of beta-sheet systems.