Influence de la nature et de l'abondance des proies sur l'augmentation des effectifs de deux coccinelles prédatrices de la cochenille du manioc au Congo

Résumé Les variations d'abondance des 2 cochenillesPhenacoccus manihoti Matile-Ferrero etFerrisia virgata Cockerell et des 2 prédateurs CoccinellidaeHyperaspis senegalensis hottentotta Mulsant etExochomus flaviventris Mader sont étudiées dans une parcelle de manioc. La colonisation de cette dernière parE. flaviventris est précoce, en présence de faibles niveaux numériques des 2 proies, mais avec une dominance deF. virgata; celle d'H. s. hottentotta intervient un mois plus tard en relation semble-t-il avec la présence d'une population deP. manihoti abondante et jeune. Nos observations font ressortir une dynamique des populations propre à chaque espèce de coccinelle, conditionnée par l'abondance de l'une ou l'autre des proies, la structure des colonies de chaque cochenille et les conditions climatiques (température) qui interviennent en synergie. Ainsi, la réponse numérique deH. s. hottentotta, plus forte que celle d'E. flaviventris, semble en relation plus étroite avecP. manihoti. PourE. flaviventris il appara?t difficile de séparer ce qui revient à chaque espèce de cochenille:F. virgata joue sans doute un r?le important pour son implantation dans les champs, puis sa raréfaction, mais c'est probablementP. manihoti qui permet l'augmentation de ses effectifs.      

Sensory receptors of the ovipositor ofTrichogramma maidis [Hym.:Trichogrammatidae]

Sensory receptors of the ovipositor ofTrichogramma maidis are described. Sense organs are found on the 2^nd valvifers (1 type), on the tip of the 3^rd valvulae (2 types) and on the 1^st valvulae (4 types). The nature and possible functions of these sensilla are discussed.      

Linkage disequilibrium and extended haplotypes in the HLA-A to D6S105 region: implications for mapping the hemochromatosis gene (HFE)

The hemochromatosis gene (HFE) maps to 6p21.3, in close linkage with the HLA Class I genes. Linkage disequilibrium (LD) studies were designed to narrow down the most likely candidate region for HFE, as an alternative to traditional linkage analysis. However, both the HLA-A and D6S105 subregions, which are situated 2–3 cM and approximately 3 Mb apart, have been suggested to contain HFE. The present report extends our previous study based upon the analysis of a large number of HFE and normal chromosomes from 66families of Breton ancestry. In addition to the previously used RFLP markers spanning the 400-kb surrounding HLA-A, we examined three microsatellites: D6S510, HLA-F, and D6S105. Our combined data not only confirm a peak of LD at D6S105, but also reveal a complex pattern of LD over the i82 to D6S105 interval. Within our ethnically well-defined population of Brittany, the association of HFE with D6S105 is as great as that with HLA-A, while the internal markers display a lower LD. Fine haplotype analysis enabled us to identify two categories of haplotypes segregating with HFE. In contrast to the vast majority of normal haplotypes, 50% of HFE haplotypes are completely conserved over the HLA-A to D6S105 interval. These haplotypes could have been conserved through recombination suppression, selective forces and/or other evolutionary factors. This particular haplotypic configuration might account for the apparent inconsistencies between genetic linkage and LD data, and additionally greatly complicates positional cloning of HFE through disequilibrium mapping.The authors contributed equally to this work     

Adaptive surface antigen variation in Mycoplasma bovis to the host immune response

Abstract The variability of predominant Mycoplasma bovis surface antigens in the presence of specific immune pressure was analyzed in an in vitro assay to determine if M. bovis could escape immune destruction. We have shown that serum antibodies from immunized or experimentally infected calves and monoclonal antibodies which specifically react with previously characterized or as yet undefined major M. bovis membrane surface proteins cause repression of expression or shortening of the target protein, or induce switching to expression of an antigenically distinct variant protein. We have further demonstrated that removal of the inducing antibody results in reversion to the original phenotype. These results suggest that the level of expression and the length of M. bovis surface antigens in the host is modulated by cognate antibodies. According to the surface antigenic variation systems, random selection of preexisting variants resistant to antibody-mediated inhibition or direct regulation of gene expression may be means by which this organism evades host immune defences.     

Core temperature variability in diving king penguins (Aptenodytes patagonicus): a preliminary analysis

Core temperature was determined in two king penguins living in the wild at Ile de la Possession, Crozel Archipelago, using implantable four-channel temperature loggers. Core temperatures derived from bird no. 1 (sensor placed under the sternum, in the vicinity of the liver and upper stomach) were closely correlated with diving activity (as determined by an external light recorder), and ranged from 38.3°C, (on land) to a minimum of 37.2°C during a dive. Core temperatures measured in bird no. 2 showed that temperatures near the heart were generally 1°C lower than those under the sternum or in the lower abdomen. Core temperatures declined continuously during dives (by 0.8, 1.2 and 2.7°C in the lower abdomen, under the sternum and near the heart, respectively) and showed precipitous drops to 35°C, probably associated with ingestion of food. Temperatures measured near the heart fluctuated over a period of 288 s, corresponding to the duration (from the literature) of the surface/dive cycle. The relevance of these findings with respect to diving physiology, blood perfusion of tissues, tissue metabolism and aerobic dive limits is discussed.     

Human neurotrophin-3: A one-step peptide mapping method and complete disulfide characterization of the recombinant protein

Human neurotrophin-3 (NT-3) is a member of the nerve growth factor (NGF) family of neurotrophic factors, and the recombinant protein is being developed as a therapeutic for neurodegenerative diseases. The final product purity and lot-to-lot variation are monitored routinely by peptide mapping. However, only the N-terminal region of NT-3 was susceptible to proteolysis under native conditions. Complete digestion required that the protein be chemically modified by reduction and S-alkylation prior to proteolysis. Complete proteolytic degradation of the protein was achieved simply by an intial denaturation of NT-3 in 6 M guanidinium chloride (pH 6) for 2 hr at 37°C, followed by a tenfold dilution with the digestion buffer (0.1 M Tris-HCl, 1 mM CaCl₂ at pH 7.0) and immediate addition of chymotrypsin at 1% by weight. Direct comparison of the peptide map with an identical aliquot that had been reduced and alkylated also allowed the establishment of the cystine linkages present in NT-3: Cys¹⁴ to Cys⁷⁹, Cys⁵⁷ to Cys¹⁰⁸, and Cys⁶⁷ to Cys¹¹⁰. This disulfide structure is homologous to the NGF family of neurotrophic factors.     

A cellular model for drug interactions on hematopoiesis: The use of human umbilical cord blood progenitors as a model for the study of drug-related myelosuppression of normal hematopoiesis

A cellular model of hematopoiesis which would be more convenient than bone marrow (BM) progenitors and directly relevant to human pathology is needed in order to investigate xenobiotic toxicity. Human umbilical cord blood (HCB), previously shown to be able to repopulate BM, provides a powerful in vitro model of normal human hematopoiesis. In order to validate the use of normal HCB progenitors as targets for dose-related myelosuppression, we used clonogenic assays and expansion in a liquid culture of progenitor-enriched cell suspensions from HCB. A series of 8 reference molecules, doxorubicin, cytosine-arabinoside, 5-fluorouracil, 3-azido-3-deoxythymidine, acetylsalicyclic acid, sodium valproate and two cephalosporin antibiotics, were tested. In vitro 50% inhibition concentrations (IC₅₀) were compared to those observed or reported with BM progenitors, and to the values of plasma concentrations from treated patients. HCB progenitors as in vitro targets for cytotoxic molecules were easy to access and handle, and their use was sensitive, specific and reproducible. They gave results similar to BM progenitors and allowed a qualitative approach to cellular metabolism and toxicity using morphological, flow cytometric and chromatographic methods.Abbreviations ARA-CC cytosine arabinoside - AS acetylsalicylic acid - AZTT 3-azido-3-deoxythymidine - BFUU burst-forming units - BM bone marrow - CFU colony-forming units - DOXX doxorubicin - FU 5-fluorouracil - glyAA glyAcophorin A - HCB human umbilical cord blood - IU international units - PCMEM human placenta-conditioned medium - VA sodium valproate     

Egg contents in relation to host-feeding in some parasitic hymenoptera

Using transmission electron microscopy, egg content is studied in 10 parasitoid species, which either feed on host hemolymph or do not. As expected, hydropic eggs were found to contain little yolk which is only lipoid. In host-feeding species, the yolk contains a great number of typical protein bodies whereas in species that do not host-feed and which produce large eggs, the yolk contain very different bodies.