全文获取类型
收费全文 | 10020篇 |
免费 | 823篇 |
国内免费 | 1篇 |
专业分类
10844篇 |
出版年
2023年 | 40篇 |
2021年 | 69篇 |
2020年 | 86篇 |
2019年 | 75篇 |
2018年 | 180篇 |
2017年 | 219篇 |
2016年 | 235篇 |
2015年 | 228篇 |
2014年 | 272篇 |
2013年 | 436篇 |
2012年 | 752篇 |
2011年 | 832篇 |
2010年 | 428篇 |
2009年 | 260篇 |
2008年 | 619篇 |
2007年 | 683篇 |
2006年 | 603篇 |
2005年 | 526篇 |
2004年 | 492篇 |
2003年 | 491篇 |
2002年 | 454篇 |
2001年 | 310篇 |
2000年 | 332篇 |
1999年 | 164篇 |
1998年 | 102篇 |
1997年 | 84篇 |
1996年 | 102篇 |
1995年 | 78篇 |
1994年 | 87篇 |
1993年 | 83篇 |
1992年 | 80篇 |
1991年 | 83篇 |
1990年 | 77篇 |
1989年 | 70篇 |
1988年 | 74篇 |
1987年 | 53篇 |
1986年 | 71篇 |
1985年 | 77篇 |
1984年 | 75篇 |
1983年 | 75篇 |
1982年 | 79篇 |
1981年 | 70篇 |
1980年 | 69篇 |
1979年 | 80篇 |
1978年 | 54篇 |
1977年 | 62篇 |
1976年 | 54篇 |
1975年 | 32篇 |
1974年 | 41篇 |
1973年 | 38篇 |
排序方式: 共有10000条查询结果,搜索用时 12 毫秒
171.
172.
M. Riedy H. Matoba N. K. Vøllestad C. R. Oakley S. Blank L. Hermansen P. D. Gollnick 《Histochemistry and cell biology》1984,80(6):553-557
Summary Biopsy samples from the vastus lateralis muscle (VLM) of man were examined for fiber composition at rest and at selected intervals
during prolonged exercise ranging in intensity from 40% to 75% of the total body maximal oxygen uptake (VO
2max) and one-min bouts of exercise at 150%VO
2max. Because of the heterogeneity of fibers in human VLM, studies were also completed where the effect of exercise on the fiber
composition of the rat soleus muscle (SM) was examined. In some animals the SM from one hindlimb was removed 9 days prior
to their being exercised after which the remaining SM was removed. Exercise reduced muscle glycogen in all experiments. In
the studies with man, blood lactate exceeded 17 mmoles/l after the heavy exercise but was largely unchanged by endurance exercise.
Colonic temperature of the exercised rats exceeded 40° C. In studies where fibers were identified only as type I and type
II, type II fibers in the VLM of all samples (16) taken at rest averaged 61.2±12.5% as compared to 59.0±12.0% after exercise
(54 biopsy samples). In a second series of studies with man where the subtypes of type II fibers were identified, there were
also no differences in fiber composition of the VLM after varying periods of exercise. Glycogen content and percent fiber
composition were the same in right and left SM obtained from rested rats. Exercise (30 to 40 min) did not alter the fiber
composition of the rat SM. These data demonstrate that the histochemically demonstratable myofibrillar actomyosin ATPase of
skeletal muscle is not altered by a single exercise bout. 相似文献
173.
In germinating spores of the parasitic fungus, Aphanomyces astaci, chitinase was first demonstrated shortly before the germ-tube began to branch, in contrast to protease which was present in both ungerminated and germinated spores. The time at which chitinase would be required when this fungus penetrates the crayfish cuticle is correlated with that of the in vitro production of chitinase. 相似文献
174.
Uptake of horseradish peroxidase from CSF into the choroid plexus of the rat,with special reference to transepithelial transport 总被引:3,自引:0,他引:3
Summary Protein uptake from cerebral ventricles into the epithelium of the choroid plexus, and transport across the epithelium were studied ultrastructurally in rats. Horseradish peroxidase (HRP, MW 40,000) was used as protein tracer. Steady-state ventriculo-cisternal perfusion with subatmospheric pressure (-10cm of water) in the ventricular system was applied. HRP dissolved in artificial CSF was perfused from the lateral ventricles to cisterna magna for various times, and ventriculo-cisternal perfusion, vascular perfusion or immersion fixation with a formaldehyde-glutaraldehyde solution was performed.Coated micropinocytic vesicles containing HRP were seen both connected with the apical, lateral and basal epithelial surface and within the cells. Heavily HRP-labeled vesicles were often fused with the lining membrane of slightly labeled or unlabeled intercellular spaces. Since the apical tight junctions of the epithelium never appeared open or never contained HRP in the spaces between the fusion points, and since the intercellular spaces between adjacent epithelial cells below the junctions only infrequently contained tracer after 5 min, by increasing amounts after 15–60 min of HRP perfusion, a vesicular transport of HRP from the apical epithelial surface to the intercellular spaces, bypassing the tight junctions, is suggested.In addition to the transepithelial transport, micropinocytic vesicles also transported HRP to the lysosomal apparatus of the epithelial cells. With increasing length of exposure to HRP, a sequence of HRP-labeled structures could be evaluated, from slightly labeled apical vacuoles and multivesicular bodies to very heavily labeled dense bodies. 相似文献
175.
Dr. Daniel Szöllösi 《Cell and tissue research》1978,191(1):115-119
Summary In polyovular, primordial follicles in the rabbit, desmosomes are found between apposing oocyte surfaces. Morphologically these desmosomes correspond closely to those described in epithelia of vertebrates. The desmosomes alternate with other junctions which are probably gap junctions.This work was supported in part by the G.R.S.T. Grant n 75.7.1313 相似文献
176.
Summary Parasympathetic stimulation of parotid glands has been studied in vivo, a) in normal resting glands, b) 72 h after post-ganglionic sympathectomy and c) after adrenergic degranulation of the acinar cells.Morphological results in each gland were compared with a similarly pretreated, but not parasympathetically stimulated, contralateral gland from the same animal.On parasympathetic stimulation of glands with densely granulated acinar cells (groups a- and b-) a variable, but usually relatively small, tendency for vacuole formation occurred in some cells. After prior degranulation of the cells (group c-) the tendency for vacuole formation was greatly accentuated. This indicates that the pre-existing metabolic state of the cells can influence the responses to stimulation of a single nerve. Dilatation of rough endoplasmic reticulum and nuclear changes were also more prominent after parasympathetic stimulation of previously degranulated acinar cells, and this suggests that parasympathetic impulses may have strong activating effects on resynthesis under these conditions. It is also likely that parasympathetic stimulation induced some, albeit small, degree of degranulation and, since this occurred in the absence of sympathetic nerves (group b-) it was probably the consequence of a direct cholinergic effect. The present results therefore indicate that the concept of an absolute dichotomy between parasympathetic and sympathetic responses is not tenable in this tissue.Analyses of saliva for amylase and peroxidase gave complex results but indicate that the two enzymes are not necessarily secreted in parallel. The morphological results support the idea that some enzyme molecules may have entered the saliva without being prepackaged into secretory granules, but could have passed directly from dilated cisterns of rough endoplasmic reticulum into intra-cellular vacuoles, and this tendency was most apparent after para-sympathetic stimulation of previously degranulated cells.Travel grants from the Wellcome Trust to J.R. Garrett are gratefully acknowledged. This work has been helped by the technical assistance of Mr. P.S.A. RowleyM.R.C. Research Assistant 相似文献
177.
Summary The permeability of fenestrated capillaries in the mouse pineal gland to proteins and peptides was demonstrated by means of ultrastructural tracers. Horseradish peroxidase (HRP) and microperoxidase (MP) were injected intravenously and allowed to circulate for approximately 30 s, 1 min, 5 min, 1 or 2h. The tissue was then fixed by vascular perfusion or by immersion with aldehydes. In all experiments a pronounced extravasation of HRP and MP occurred. Transendothelial vesicular transport seemed to have occurred across the fenestrated capillaries. The most pronounced tracer labeling of vesicles was found after 1 min of MP- or HRP-circulation. The vesicles were uncoated and more than 70 % of the HRP-and MP-containing vesicles exhibited diameters between 50 and 110 nm. Furthermore, three other transcapillary pathways taken by the tracers are suggested: 1) via intercellular junctions, 2) through fenestrae and 3) via channels formed by fusion of vesicles with the luminal and abluminal cell membranes. Based on these results, it is assumed that the capillaries in the mouse pineal gland are also permeable to peptides synthesized and secreted by the pineal gland.Part of this study was presented at the EMCELL-76 meeting, Copenhagen, 1976 相似文献
178.
Ulrik Merrild Vibeke Schiøler Flemming Christensen Ewald Wolny Carl Johan Edeling 《Human genetics》1978,45(1):85-88
Summary Elevated levels of alpha-1-fetoprotein (AFP) were found in the amniotic fluid of a 36-year-old woman in the 15th week of gestation. Because of this and the results of repeated ultrasonography, abortion was induced. An anencephalic fetus with trisomy 18 was delivered. The possible correlation among neural-tube defects, chromosomal abnormalities, and level of AFP is discussed. 相似文献
179.
Søren Wilken Rasmussen 《Chromosoma》1977,60(3):205-221
In Bombyx mori oocytes the synaptonemal complexes are retained in modified form from pachytene to metaphase I. At the end of pachytene the length and width of the lateral components of the complex increase, whereafter the complexes become compacted during later stages of the meiotic prophase. Ultimately, at metaphase I the modified synaptonemal complexes of individual bivalents fuse to form a more or less continuous sheet between the homologous chromosomes. This sheet corresponds to the structure historically known as the elimination chromatin. It is concluded that in the absence of crossing over and chiasma formation in Bombyx mori females the retainment and subsequent modification of the synaptonemal complex has evolved as a substitute mechanism to ensure regular disjunction of the bivalents. 相似文献
180.
P. O. Fanger J. Højbjerre J. O. B. Thomsen 《International journal of biometeorology》1977,21(1):44-50
Preferred room temperatures were determined for 16 winter swimmers who had been exposed to cold shock daily by bathing in the sea (0–6°C) during at least two winter seasons. Each subject participated in one comfort experiment (2 1/2 h) in an environmental test chamber, where the preferred ambient temperature was determined by adjusting it according to his wishes. The subjects were sedentary and clothed in a standard uniform. Skin temperatures, rectal temperature and evaporative weight loss were measured. The results were compared with similar data for two other groups: (a) a control group of 64 normal subjects and (b) 16 persons who had been working 8 h a day at 8°C during at least 6 months in the meat packing industry. The results showed no significant differences between the ambient temperatures preferred by the three groups of subjects. This indicated that man cannot be adapted to prefer lower ambient temperatures by exposing him daily to cold. 相似文献