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41.
Blanco A Simeone R Gadaleta A 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(7):1195-1204
Grain protein content (GPC) of durum wheat (Triticum turgidum L. var. durum) is an important trait for the nutritional value of grain and for influencing the technological property of flour. Protein
content is a quantitative trait negatively correlated with grain yield, thus increase in protein quantity usually results
in yield reduction. This study was initiated to introgress alleles for high GPC from var. dicoccoides into durum wheat germplasm by the backcross inbred line (BIL) method and to identify molecular markers linked to high GPC
alleles not associated with depressing effects on yield. The backcross line 3BIL-85 with high GPC and similar grain yield
to the recurrent parent was backcrossed to Latino, and the generations F2, F3 and F4 were evaluated for GPC and yield per spike (GYS) in three field trials. Three QTLs with major effects on GPC were detected
on chromosome arms 2AS, 6AS and 7BL, identified by the markers Xcfa2164, XP39M37
(250)
and Xgwm577
, respectively. Multiple regression analysis indicated that the three QTLs explained all the genetic variances of the trait.
The high GPC parental line 3BIL-85 was not significantly different from the recurrent parent Latino for GYS, but the phenotypic
correlation coefficient between GPC and GYS had negative values (from −0.02 to −0.28) in each trial, although it was statistically
significant only in the F3 progeny trial. No co-located QTL for GYS was detected, excluding the hypothesis that the putative
QTLs for GPC were indirect QTLs for low grain yield. The negative protein-yield response could be due to: (a) co-location
of grain yield per spike QTLs with reduced phenotypic effects not detectable by the experimental design or statistical procedures,
or to (b) opposite pleiotropic gene effects due to the major bio-energetic requirements for synthesis of protein then carbohydrates.
Mapping loci by BILs should enable the production of near-isogenic lines in which the individual effects of each QTL can be
examined in detail without confounding variations due to other putative QTLs.
An erratum to this article can be found at 相似文献
42.
Lezza AM Fallacara FP Pesce V Leeuwenburgh C Cantatore P Gadaleta MN 《Neurochemical research》2008,33(12):2609-2614
According to the “mitochondrial theory of aging” the lifelong accumulation of various kinds of damage to mitochondrial DNA
(mtDNA) has been related to the age-dependent mitochondrial bioenergetic dysfunction. Caloric restriction (CR) diet is able
to prevent or delay the onset of several age-related damages to mtDNA. The effects of aging and CR on the presence of abasic
sites and single-strand breaks of the sugar–phosphate backbone in mtDNA have been analyzed by applying Ligation Mediated-PCR
to a H strand region of brain mtDNA from young and old ad libitum-fed and old CR-treated rats. The region, encompassing the
Direct Repeat 1 of the 4,834 bp-long deletion, is highly damaged in the old ad libitum-fed animals with respect to the young
ones, whereas in the CR rats it shows a much lower extent of damage. The data confirm, at single nucleotide resolution, the
protective effect of CR on the age-related mtDNA damage.
Special issue article in honor of Dr. Anna Maria Giuffrida-Stella. 相似文献
43.
Moriggi M Cassano P Vasso M Capitanio D Fania C Musicco C Pesce V Gadaleta MN Gelfi C 《Proteomics》2008,8(17):3588-3604
After hind limb suspension, a remodeling of postural muscle phenotype is observed. This remodeling results in a shift of muscle profile from slow-oxidative to fast-glycolytic. These metabolic changes and fiber type shift increase muscle fatigability. Acetyl-L-carnitine (ALCAR) influences the skeletal muscle phenotype of soleus muscle suggesting a positive role of dietary supplementation of ALCAR during unloading. In the present study, we applied a 2-D DIGE, mass spectrometry and biochemical assays, to assess qualitative and quantitative differences in the proteome of rat slow-twitch soleus muscle subjected to disuse. Meanwhile, the effects of ALCAR administration on muscle proteomic profile in both unloading and normal-loading conditions were evaluated. The results indicate a modulation of troponin I and tropomyosin complex to regulate fiber type transition. Associated, or induced, metabolic changes with an increment of glycolytic enzymes and a decreased capacity of fat oxidation are observed. These metabolic changes appear to be counteracted by ALCAR treatment, which restores the mitochondrial mass and decreases the glycolytic enzyme expression, suggesting a normalization of the metabolic shift observed in unloaded animals. This normalization is accompanied by a maintenance of body weight and seems to prevent a switch of fiber type. 相似文献
44.
45.
Masanori?Nakaem-nakae@cc.kochi-u.ac.jp; KS fishssk@cc.kochi-u.ac.jp" title="MN m-nakae@cc.kochi-u.ac.jp; KS fishssk@cc.kochi-u.ac.jp" itemprop="email" data-track="click" data-track-action="Email author" data-track-label="">Email author Kunio?Sasaki 《Ichthyological Research》2004,51(4):327-336
Homologies of the adductor mandibulae muscles in eight families of Tetraodontiformes were hypothesized from the branching patterns of ramus mandibularis trigeminus. Insertions of the muscles to the upper or lower jaw were weak indicators of homology, migrations of the sites occurring frequently in A1, A2, A2, and A3. In monacanthids, tetraodontids, and diodontids, A1 tended to be split into numerous subsections, whereas in aracanids and ostraciids, A3 was highly developed, comprising three or four subsections. In tetraodontids, A2 was found to be a composite of A1 subsection and A2. The methods of and limits to applying nerve branching patterns to muscle homology are discussed. A new naming system that reflects both muscle homologies and insertions is proposed. 相似文献
46.
47.
48.
A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli 总被引:1,自引:0,他引:1
Patricia Gadaleta Sara Kaufman Patricia Martini Jorge Zorzópulos 《FEMS microbiology letters》1991,80(2-3):147-150
Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Apr), gentamicin (Gmr) and amikacin (Akr) resistance was transformed into Escherichia coli. Transformation efficiency was about 2 x 10(3) transformants/micrograms of plasmid DNA. The plasmids present in the E. coli transformants were identical to pPG1 according to their restriction patterns. The copy number of pPG1 was estimated to be at least 20-times less in E. coli than in S. aureus. The minimal inhibitory concentrations (MICs) for Ap and Gm were lower in E. coli than in S. aureus. However, the MIC for Ak was higher in E. coli transformants than in S. aureus. pPG1 was maintained in the E. coli transformants for at least 80 generations at 37 degrees C without antibiotic selection pressure. 相似文献
49.
50.
Patruno R Arpaia N Gadaleta CD Passantino L Zizzo N Misino A Lucarelli NM Catino A Valerio P Ribatti D Ranieri G 《Journal of cellular and molecular medicine》2009,13(3):555-561
Canine cutaneous mast cell tumour (CMCT) is a common cutaneous tumour in dog, with a higher incidence than in human. CMCT is classified in three subgroups, well and intermediately differentiated (G1 and G2), corresponding to a benign disease, and poorly differentiated (G3), corresponding to a malignant disease, which metastasize to lymph nodes, liver, spleen and bone marrow. In this study, we have evaluated serum (S), platelet-poor plasma (P-PP), plasma-activated platelet rich (P-APR) and cytosol vascular endothelial growth factor (VEGF) concentrations, microvascular density (MVD) and mast cell density (MCD) in a series of 86 CMCTs and we have correlated these parameters with each other, by means of ELISA detection of VEGF and immunohistochemistry. Results show that VEGF level from cytosol P-APR and MVD were significantly higher in G3 CMCTs as compared to G1 or G2 subgroups. Moreover, a significantly strong correlation among VEGF levels from P-PAR and cytosol, MVD and MCD was found in G3 subgroup. Because VEGF levels from P-APR well correlated with MVD and malignancy grade in CMCT, we suggest that VEGF might be secreted from MCs and it may be a suitable surrogate inter-species angiogenetic markers of tumour progression in CMCT. Finally, CMCT seems to be a useful model to study the role of MCs in tumour angiogenesis and inhibition of MCs degranulation or activation might be a new anti-angiogenic strategy worthy to further investigations. 相似文献