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991.
Gerson Chadi Jessica Ruivo Maximino Gabriela Pintar de Oliveira 《Journal of molecular histology》2009,40(4):241-250
Neuron-glia interaction is involved in physiological function of neurons, however recent evidences have suggested glial cells
as participants in neurotoxic and neurotrophic mechanisms of neurodegenerative/neuroregenerative processes. Histological techniques
employing immunolabeling, historadiography and in situ hybridization have been useful to localize at cell levels molecules
in normal and pathological situations. The intercellular accomplishment leading to neuronal injury in central nervous system
disorders implies the performance of quantitative assays to better interpret the role of related molecules or signal pathways,
however one limitation employing the whole tissue is the loss of cellular resolution. The laser capture microdissection was
developed recently and allows the selection of specific cell types from their original environment after freezing and sectioning
the tissue sampling, leading to the quantification of gene expression in individual cells, thus providing a unique opportunity
to get new informations on cell signaling related to neurodegeneration. Here we reviewed the role of glial cell signaling
on neurodegenerative disorders like ischemia, Parkinson and Alzheimer diseases, and also amyotrophic lateral sclerosis and
what has been published with regards to single cell laser capture microdissection technique in the molecular biology investigation
on these issues. 相似文献
992.
Paulo Cavalheiro Schenkel Angela Maria Vicente Tavares Rafael Oliveira Fernandes Gabriela Placoná Diniz Mariane Bertagnolli Alex Sander da Rosa Araujo Maria Luiza Barreto-Chaves Maria Flavia Marques Ribeiro Nadine Clausell Adriane Belló-Klein 《Molecular and cellular biochemistry》2010,341(1-2):1-8
In this study, we investigated the oxidative stress influence in some prosurvival and proapoptotic proteins after myocardial infarction (MI). Male Wistar rats were divided in two groups: Sham-operated (control) and MI. MI was induced by left coronary artery occlusion. 28-days after surgery, echocardiographic, morphometric, and hemodynamic parameters were evaluated. Redox status (reduced to oxidized glutathione ratio, GSH/GSSG) and hydrogen peroxide levels (H2O2) were measured in heart tissue. The p-ERK/ERK, p-Akt/Akt, p-mTOR/mTOR and p-GSK-3β/GSK-3β ratios, as well as apoptosis-inducing factor (AIF) myocardial protein expression were quantified by Western blot. MI group showed an increase in cardiac hypertrophy (23%) associated with a decrease in ejection fraction (38%) and increase in left ventricular end-diastolic pressure (82%) when compared to control, characterizing ventricular dysfunction. Redox status imbalance was seen in MI animals, as evidenced by the decrease in the GSH/GSSG ratio (30%) and increased levels of H2O2 (45%). This group also showed an increase in the ERK phosphorylation and a reduction of Akt and mTOR phosphorylation when compared to control. Moreover, we showed a reduction in the GSK-3β phosphorylation and an increase in AIF protein expression in MI group. Taken together, our results show increased H2O2 levels and cellular redox imbalance associated to a higher p-ERK and AIF immunocontent, which would contribute to a maladaptive hypertrophy phenotype. 相似文献
993.
Al-Kaddah S Reder-Christ K Klocek G Wiedemann I Brunschweiger M Bendas G 《Biophysical chemistry》2010,152(1-3):145-152
The interaction of the lantibiotic gallidermin and the glycopeptide antibiotic vancomycin with bacterial membranes was simulated using mass sensitive biosensors and isothermal titration calorimetry (ITC). Both peptides interfere with cell wall biosynthesis by targeting the cell wall precursor lipid II, but differ clearly in their antibiotic activity against individual bacterial strains. We determined the binding affinities of vancomycin and gallidermin to model membranes±lipid II in detail. Both peptides bind to DOPC/lipid II membranes with high affinity (K(D) 0.30 μM and 0.27 μM). Gallidermin displayed also strong affinity to pure DOPC membranes (0.53 μM) an effect that was supported by ITC measurements. A surface acoustic wave (SAW) sensor allowed measurements in the picomolar concentration range and revealed that gallidermin targets lipid II at an equimolar ratio and simultaneously inserts into the bilayer. These results indicate that gallidermin, in contrast to vancomycin, combines cell wall inhibition and interference with the bacterial membrane integrity for potent antimicrobial activity. 相似文献
994.
In this study mycorrhizal fungi were isolated from the roots of the endemic terrestrial orchid Bipinnula fimbriata. Seven isolates were previously identified as the form-genus Rhizoctonia, a polyphyletic group known to form mycorrhizal associations with Orchidaceae. Two other isolates were included in the study: #793 isolated from Chloraea crispa, and #1325 Rhizoctonia solani, isolated from potato. After morphological and molecular characterization of the nine isolates, they were divided into three groups, Ceratobasidium sp., Tulasnella calospora and Thanatephorus cucumeris, to determine the diversity between isolates. Consensus ITS sequences were used for a blast search on the GenBank database, which confirmed the results of the morphological observations. Once the isolates were identified, an in vitro germination test was done with four plates of oatmeal agar inoculated with each fungus, plus an asymbiotic control. The germination stages of the seeds were recorded 30 days after sowing. All isolates obtained from B. fimbriata, and the isolate #793 from Chloraea crispa, promoted seed germination. However, the isolate #1325 Rhizoctonia solani, which is known as both a pathogen and an orchid symbiont, did not promote germination. This shows that B. fimbriata is associated with more than one mycorrhizal fungus in its habitat and has a broader potential specificity in vitro. The results support the hypothesis that at least one fungal isolate promotes the germination of B. fimbriata, permitting the conservation of this species in ex situ conditions. 相似文献
995.
Gabriela Montero-Moran Jorge M. Caviglia Derek McMahon Alexis Rothenberg Vidya Subramanian Zhi Xu Samuel Lara-Gonzalez Judith Storch George M. Carman Dawn L. Brasaemle 《Journal of lipid research》2010,51(4):709-719
Mutations in human CGI-58/ABHD5 cause Chanarin-Dorfman syndrome (CDS), characterized by excessive storage of triacylglycerol in tissues. CGI-58 is an α/β-hydrolase fold enzyme expressed in all vertebrates. The carboxyl terminus includes a highly conserved consensus sequence (HXXXXD) for acyltransferase activity. Mouse CGI-58 was expressed in Escherichia coli as a fusion protein with two amino terminal 6-histidine tags. Recombinant CGI-58 displayed acyl-CoA-dependent acyltransferase activity to lysophosphatidic acid, but not to other lysophospholipid or neutral glycerolipid acceptors. Production of phosphatidic acid increased with time and increasing concentrations of recombinant CGI-58 and was optimal between pH 7.0 and 8.5. The enzyme showed saturation kinetics with respect to 1-oleoyl-lysophosphatidic acid and oleoyl-CoA and preference for arachidonoyl-CoA and oleoyl-CoA. The enzyme showed slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid. Recombinant CGI-58 showed intrinsic fluorescence for tryptophan that was quenched by the addition of 1-oleoyl-lysophosphatidic acid, oleoyl-CoA, arachidonoyl-CoA, and palmitoyl-CoA, but not by lysophosphatidyl choline. Expression of CGI-58 in fibroblasts from humans with CDS increased the incorporation of radiolabeled fatty acids released from the lipolysis of stored triacylglycerols into phospholipids. CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids. 相似文献
996.
997.
Yolanda Karla Cupertino da Silva Cristina Villarinho Augusto Maria Letícia de Castro Barbosa Gabriela Muniz de Albuquerque Melo Aline Cavalcanti de Queiroz Thays de Lima Matos Freire Dias Walfrido Bispo Júnior Eliezer J. Barreiro Lídia Moreira Lima Magna Suzana Alexandre-Moreira 《Bioorganic & medicinal chemistry》2010,18(14):5007-5015
In this paper, we report the synthesis and pharmacological evaluation of pyrazine N-acylhydrazone (NAH) derivatives (2a–s) designed as novel analgesic and anti-inflammatory drug candidates. This series was planned by molecular simplification of prototype 1 (LASSBio-1018), previously described as a non-selective cyclooxygenase inhibitor. Derivatives 2a–s were evaluated in several animal models of pain and inflammation, standing-out compound 2o (2-N′-[(E)-(3,4,5-trimethoxyphenyl) methylidene]-2-pyrazinecarbohydrazide; LASSBio-1181), that was also active in a murine model of chronic inflammation (i.e., adjuvant-induced arthritis test in rats) and can be considered a new analgesic and anti-inflammatory lead for drug development. 相似文献
998.
Gabriela Solano Álvaro Segura María Herrera Aarón Gómez Mauren Villalta José María Gutiérrez Guillermo León 《Biologicals》2010,38(5):577-585
The lethality neutralization assay performed in mice is the standard recommended by the World Health Organization to estimate antivenom potency. The interpretation of its results without considering its analytical capacity may lead to erroneous conclusions. Therefore, laboratories that manufacture or control antivenoms must demonstrate the appropriateness of their models. A study of the method used at Instituto Clodomiro Picado, Costa Rica, to estimate the potency of antivenoms against Bothrops asper snake venom was performed. Results show that venom doses ranging from 2 to 6 Median Lethal Doses (LD50) are appropriate to be used as challenge in this test. Variables such as the injection route, number of mice used per venom/antivenom level, and weight of the animals are critical in the estimation of the Median Effective Dose (ED50), whereas incubation time is not. The assay has an acceptable selectivity, linearity, and limits of detection and quantification. Accuracy of the lethality neutralization assay, expressed as percentage recovery, was between 71% and 127%. Intermediate precision, expressed as relative standard deviation, was ≤17%. It is concluded that the analytical characteristics of this assay are adequate enough to prove product compliance and to have statistical control over an industrial line of antivenom serial production. 相似文献
999.
Gabriela Pérez-Lachaud José Antonio López-Méndez Guy Beugnon Peter Winterton Jean-Paul Lachaud 《Biological Control》2010,52(2):131-139
Composition and dynamics of ant communities may be influenced by highly specialized, specific parasitoids such as eucharitids. Yet, little is known about their prevalence in ant societies. Through systematic monthly excavation of ant nests, we evaluated the impact on the Neotropical ant Ectatomma tuberculatum of two eucharitid parasitoid species, Dilocantha lachaudii and Isomerala coronata, which simultaneously attack the same host populations in southern Mexico. Nearly 90% of all the nests collected through the year were parasitized, with an average of 13% ant pupae and 6.7% ant larvae parasitized by eucharitids, and an annual loss of 17% of the ant brood. Eucharitid prevalence among host nests was, however, very variable, and only some E. tuberculatum nests were severely weakened (100% of ant brood parasitized). Parasitism was highest during the dry season (January–March), just when the production of ant pupae was minimum: up to 50.6% of the ant pupae were destroyed in March. However, production of E. tuberculatum males and females occurred later (June–July), and the reproductive potential of the host colonies did not ultimately seem to be heavily affected by eucharitid parasitism. Differences in the seasonal timing of eucharitid attack and ant reproduction thus have the potential to modulate the impact of eucharitids on ants. Our results are discussed in the context of the impact of eucharitids upon E. tuberculatum colonies and their possible effect on the community structure of this potential biocontrol agent ant. 相似文献
1000.
Pablo Corcuera Pedro Luis Valverde José Alejandro Zavala-Hurtado Gabriela De la Rosa César Gabriel-Durán 《Journal of Insect Conservation》2010,14(6):711-719
Eucalyptus spp. are commonly planted, forming non-native plantations, including the tropics and their wildlife conservation value is
relatively unknown. Recent studies have concluded that secondary forests and tree plantations are less diverse than well-developed
tropical rain forests. However, introduced Eucalyptus stands harbored similar species richness to surrounding native woodland in temperate woodlands in North America though the
identity of the species present may differ. Species composition, as well as dominance curves and differences in community
structure add additional insight to understanding faunistic responses to replacement of native woodland by Eucalyptus plantations. Here, we compared species richness, diversity patterns, and the distribution of non-weaving spiders between
native woodlands and Eucalyptus plantations in a temperate region of Mexico. We found more Lycosidae species in all plantation stands. Other community attributes were not consistently different between plantations and native
woodlands. This is explained by similarities between, and differences within, the understory of the two main vegetation types.
Multivariate analyses identified three spider groups and five spider species could be identified as indicators of these groups.
A comparison of the number of species of the wandering spiders between the two vegetation types suggests a compensation pattern
that is reported here for the first time. 相似文献