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61.
Maria Lucia Taylor Guillermo M. Ruíz-Palacios María del Rocío Reyes-Montes Gabriela Rodríguez-Arellanes Laura E. Carreto-Binaghi Esperanza Duarte-Escalante Aurora Hernández-Ramírez Armando Pérez Roberto O. Suárez-Alvarez Yuri A. Roldán-Aragón Rafael Romero-Martínez Jorge H. Sahaza-Cardona José Sifuentes-Osornio Luis E. Soto-Ramírez Gabriela R. Peña-Sandoval 《FEMS immunology and medical microbiology》2013,1830(3):435-441
Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel’s ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92–99%) between them. 相似文献
62.
Elham Khanicheh Martina Mitterhuber Lifen Xu Stéphanie P. Haeuselmann Gabriela M. Kuster Beat A. Kaufmann 《PloS one》2013,8(3)
Background/Objectives
Inflammatory changes on the endothelium are responsible for leukocyte recruitment to plaques in atherosclerosis. Noninvasive assessment of treatment-effects on endothelial inflammation may be of use for managing medical therapy and developing novel therapies. We hypothesized that molecular imaging of vascular cell adhesion molecule-1 (VCAM-1) with contrast enhanced ultrasound (CEU) could assess treatment effects on endothelial phenotype in early atherosclerosis.Methods
Mice with atherosclerosis produced by gene deletion of the LDL-receptor and Apobec-1-editing protein were studied. At 12 weeks of age, mice received 8 weeks of regular chow or atorvastatin-enriched chow (10 mg/kg/day). At 20 weeks, CEU molecular imaging for aortic endothelial VCAM-1 expression was performed with VCAM-1-targeted (MBVCAM) and control microbubbles (MBCtr). Aortic wall thickness was assessed with high frequency ultrasound. Histology, immunohistology and Western blot were used to assess plaque burden and VCAM-1 expression.Results
Plaque burden was reduced on histology, and VCAM-1 was reduced on Western blot by atorvastatin, which corresponded to less endothelial expression of VCAM-1 on immunohistology. High frequency ultrasound did not detect differences in aortic wall thickness between groups. In contrast, CEU molecular imaging demonstrated selective signal enhancement for MBVCAM in non-treated animals (MBVCAM 2±0.3 vs MBCtr 0.7±0.2, p<0.01), but not in statin-treated animals (MBVCAM 0.8±0.2 vs MBCtr 1.0±0.2, p = ns; p<0.01 for the effect of statin on MBVCAM signal).Conclusions
Non-invasive CEU molecular imaging detects the effects of anti-inflammatory treatment on endothelial inflammation in early atherosclerosis. This easily accessible, low-cost technique may be useful in assessing treatment effects in preclinical research and in patients. 相似文献63.
Marcelo Magioli Katia Maria Paschoaletto Micchi de Barros Ferraz Eleonore Zulnara Freire Setz Alexandre Reis Percequillo Michelle Viviane de Sá Santos Rondon Vanessa Villanova Kuhnen Mariana Cristina da Silva Canhoto Karen Evelyn Almeida dos Santos Claudia Zukeran Kanda Gabriela de Lima Fregonezi Helena Alves do Prado Mitra Katherina Ferreira Milton Cezar Ribeiro Priscilla Marqui Schmidt Villela Luiz Lehmann Coutinho Márcia Gonçalves Rodrigues 《European Journal of Wildlife Research》2016,62(4):431-446
64.
Gabriela Castaño-Meneses 《Arthropod-Plant Interactions》2016,10(5):403-417
The canopy of forests has been considered “the last biotic frontier,” and study of its elements is very important in explaining the global functionality in ecosystems. Epiphytic plants and arthropods are essential elements in canopy habitats, and their relationships have been studied in order to understand the high diversity in tropical forests. Nevertheless, there are few studies on this development in temperate forests. The arthropod community was studied during the rainy and dry seasons at two altitudes, and a total of 240 T. violacea plants of three sizes were collected from Abies religiosa and Quercus spp. host trees. A total of 163,043 arthropods were collected and about 200 morphospecies identified. The highest abundance was obtained during the dry season, while high diversity was found during the rainy season. There was a significant effect of plant size, host trees and collecting season on abundance and diversity, and there were seasonal variations in community composition. The community hosted on A. religiosa epiphytes showed higher abundance and density than that of Quercus. 相似文献
65.
Rafael Conceição de Souza Gabriela de Medeiros Muniz Andrei Santos Siqueira Adonis de Melo Lima Alessandra Pereira da Silva Evonnildo Costa Gonçalves João Lídio da Silva Gonçalves Vianez Júnior 《Journal of molecular modeling》2016,22(11):269
Human immunodeficiency virus (HIV) infections continue to exert an enormous impact on global human health. This led experts to emphasize the importance of new measures for preventing HIV infections, including the development of vaccines and novel drugs. In this context, a promising approach involves the use of lectins that can bind the surface envelope glycoprotein gp120 of HIV with high affinity, preventing viral entry. The cyanobacterial lectin microvirin (MVN) has been proposed as a candidate for development as a topical microbicide because of its ability to bind to high mannose-type glycans, potently inhibiting HIV-1 entry. Thus, the aim of this computational study was to investigate the effects of four point mutations (D53Q, D53E, D53K, and D53W) on the structure and affinity of MVN with di-mannose (MAN). Molecular dynamics simulations followed by binding free energy calculations using MM-GBSA were employed. The calculated binding free energy of ligand-receptor complexation of MVN with MAN was ?26.02 kcal mol-1. We identified in the wild-type protein that residues I45, T59, and Q81 have a major contribution to the binding free energy of di-mannose. Among the investigated mutants, the most promising one was the D53W mutation, with a theoretical binding free energy value of ?29.16 kcal mol-1. We suggest that this increased stability is due to the introduction of extra rigidity on the hinge region connecting two key structural elements of the MVN binding site. 相似文献
66.
67.
Chiara V. Segré Silvia Senese Sara Loponte Stefano Santaguida Paolo Soffientini Gabriela Grigorean 《MABS-AUSTIN》2016,8(1):37-42
Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells. 相似文献
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70.
Cancer is a worldwide increasing burden and its therapy is often challenging and causes severe side effects in healthy tissue. If drugs are loaded into nanoparticles, side effects can be reduced, and efficiency can be increased via the enhanced permeability and retention effect. This effect is based on the fact that nanoparticles with sizes from 10 to 200 nm can accumulate in tumor tissue due to their leaky vasculature. In this work, we produced polycaprolactone (PCL) in the sizes 1.8, 5.4, and 13.6 kDa and were able to produce spherical shaped nanoparticles with mean diameters of 64 ± 19 nm out of the PCL5.4 and 45 ± 8 nm out of the PCL13.6 reproducibly. By encapsulation of paclitaxel the diameter of that nanoparticles did not increase, and we were able to encapsulate 73 ± 7 fmol paclitaxel per 1000 particles in the PCL5.4‐nanoparticles and 35 ± 8 fmol PTX per 1000 PCL13.6‐nanoparticles. Furthermore, we coupled the aptamer S15 to preformed PCL5.4‐nanoparticles resulting in particles with a hydrodynamic diameter of 153 nm. This offers the opportunity to use these nanoparticles for targeted drug delivery. 相似文献