全文获取类型
收费全文 | 3204篇 |
免费 | 191篇 |
国内免费 | 1篇 |
出版年
2024年 | 7篇 |
2023年 | 29篇 |
2022年 | 62篇 |
2021年 | 120篇 |
2020年 | 77篇 |
2019年 | 83篇 |
2018年 | 97篇 |
2017年 | 84篇 |
2016年 | 116篇 |
2015年 | 190篇 |
2014年 | 181篇 |
2013年 | 234篇 |
2012年 | 275篇 |
2011年 | 255篇 |
2010年 | 161篇 |
2009年 | 135篇 |
2008年 | 152篇 |
2007年 | 184篇 |
2006年 | 169篇 |
2005年 | 145篇 |
2004年 | 146篇 |
2003年 | 101篇 |
2002年 | 96篇 |
2001年 | 26篇 |
2000年 | 20篇 |
1999年 | 23篇 |
1998年 | 29篇 |
1997年 | 14篇 |
1996年 | 8篇 |
1995年 | 8篇 |
1994年 | 9篇 |
1993年 | 9篇 |
1992年 | 10篇 |
1991年 | 18篇 |
1990年 | 12篇 |
1989年 | 16篇 |
1988年 | 9篇 |
1987年 | 10篇 |
1984年 | 6篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1979年 | 4篇 |
1978年 | 8篇 |
1975年 | 7篇 |
1974年 | 7篇 |
1973年 | 5篇 |
1971年 | 3篇 |
1941年 | 3篇 |
1940年 | 3篇 |
1939年 | 3篇 |
排序方式: 共有3396条查询结果,搜索用时 15 毫秒
31.
Biodegradation of azo and heterocyclic dyes by Phanerochaete chrysosporium. 总被引:10,自引:1,他引:9
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Biodegradation of Orange II, Tropaeolin O, Congo Red, and Azure B in cultures of the white rot fungus, Phanerochaete chrysosporium, was demonstrated by decolarization of the culture medium, the extent of which was determined by monitoring the decrease in absorbance at or near the wavelength maximum for each dye. Metabolite formation was also monitored. Decolorization of these dyes was most extensive in ligninolytic cultures, but substantial decolorization also occurred in nonligninolytic cultures. Incubation with crude lignin peroxidase resulted in decolorization of Azure B, Orange II, and Tropaeolin O but not Congo Red, indicating that lignin peroxidase is not required in the initial step of Congo Red degradation. 相似文献
32.
A single mutation in the oli2 region of the mitochondrial DNA causes a charge alteration in a mitochondrially translated subunit of the mitochondrial ATPase (subunit 6; apparent Mr 20 000; apparent pI 6.9 and 7.1). This alteration leads to the defective assembly of the proteolipid subunit into the enzyme complex. The mutant, which is able to grow only very slowly by oxidative metabolism at 28°C offers new possibilities for studying the assembly of the membrane sector (F0) into the mitochondrial ATPase complex and the role of subunit 6 in this process. 相似文献
33.
A tertiary amine monoxygenase from a Pseudomonas sp. was partially purified (35-fold) and characterized. In the presence of nitrilotriacetate (NTA), O2, NADH, and Mn2+, the enzyme yielded two sets of products: iminodiacetate, glyoxylate, NAD+ and H2O; or H2O2 and NAD+. Which set of products predominated was a function of enzyme concentration, ionic strength of solution, pH, and cation supplied. NTA functioned both as a modifiable substrate and as a stimulator of NADH oxidase activity. A requirement for preincubation with Mn2+ and NTA to eliminate enzyme hysteresis and the similar Km values for NTA and Mn2+ suggested that the substrate and metal were bound as a unit by the enzyme. 相似文献
34.
35.
36.
Gabriela Vizárová 《Biologia Plantarum》1975,17(5):380-382
The excretion of cytokinins into the cultivating medium, which are produced by the phytopathogenic fungiMonilia sp. andCytospora sp. has been investigated. All the isolates of the fungi used in the experiments (Monilia fructicola, Monilia fructigena, isolate 2 and 4,Monilia laxa isolate 3 andCytospora sp. isolate CPL and C1) have been found to produce cytokinins. The production is increased during the formation of the fructification organs. Among the isolates investigatedMonilia fructigena isolate 4 andCytospora sp. isolate CPL showed the highest production of cytokinins. After chromatographic separation, cytokinin activity was found at RF 0.7–0.8 values by the biological test as well as by identification according to UV spectra. Application of purified cytokinins produced by the fungi evoked the formation of “green islands” on isolated barley leaves underin vitro conditions. 相似文献
37.
38.
Timothy Spellman Malka Svei Jesse Kaminsky Gabriela Manzano-Nieves Conor Liston 《Cell》2021,184(10):2750-2766.e17
- Download : Download high-res image (152KB)
- Download : Download full-size image
39.
40.
Tribelli Paula M. Pezzoni Magdalena Brito María Gabriela Montesinos Nahuel V. Costa Cristina S. López Nancy I. 《Extremophiles : life under extreme conditions》2020,24(2):265-275
Extremophiles - Pseudomonas extremaustralis is an Antarctic bacterium with high stress resistance, able to grow under cold conditions. It is capable to produce polyhydroxyalkanoates (PHAs) mainly... 相似文献