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11.
Summary
Drosophila melanogaster males heterozygous for the second chromosome locus Segregation Distorter preferentially transmit this chromosome to their progeny due to a dysfunctioning of SD
+-bearing sperm. SD males with a normal sex chromosome constitution produce more females than males among SD
+ progeny. This report shows that this unequal recovery of sexes is enhanced from XY/Y; SD/SD
+ males and enhanced still further from XY/O; SD/SD
+ males. It is argued that the probability that a SD
+-bearing sperm will dysfunction is related to its sex chromsome complement, with the relative probabilities of dysfunction ranked O>
Y>
X>
XY. It is shown that a modified probit analysis accounts for the relationship between sex ratio and second chromosome segregation frequency for all paternal genotypes. Finally, SD/SD
+ males show no increase in sex chromosome nondisjunction with respect to a control.R. E. Denell was supported by U.S.P.H.S. Training Grant No. GM00337 and by a U.S.P.H.S. Postdoctoral Fellowship; George L. Gabor Miklos was supported by A.E.C. Contract No. AT (04-3)-34 PA150. 相似文献
12.
Studies on the existence of a pathway in liver and muscle for the conversion of glucose into glycogen without glucose 6-phosphate as an intermediate 总被引:1,自引:1,他引:0
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Gabor J. Antony Indira Srinivasan Hollis R. Williams Bernard R. Landau 《The Biochemical journal》1969,111(4):453-459
Mixtures of (14)C-labelled glucose plus pyruvate were incubated either with rat diaphragm or slices of rat liver. Incorporation of glucose carbon into glycogen was compared with its incorporation into glucose 6-phosphate relative to the incorporation of pyruvate carbon into these metabolic products. There was no preferential incorporation of glucose carbon relative to pyruvate carbon into glycogen compared with glucose 6-phosphate in the liver slices, but there was in diaphragm. On the assumption that glucose 6-phosphate is a necessary intermediate in the conversion of pyruvate carbon into glycogen, this is evidence for the existence in muscle, but not in liver, of more than one pool of glucose 6-phosphate or of a pathway from glucose to glycogen without glucose 6-phosphate as an intermediate. Galactose carbon, relative to pyruvate carbon, was preferentially incorporated into liver glycogen, so that a substrate converted in liver into glycogen without glucose 6-phosphate as an intermediate could be detected by this approach. 相似文献
13.
14.
Nutrient additions to wetlands in the Interlake region of Manitoba,Canada: effects of a single pulse addition in spring 总被引:3,自引:3,他引:0
Gabor T. S. Murkin H. R. Stainton M. P. Boughen J. A. Titman R. D. 《Hydrobiologia》1994,279(1):497-510
This study examined the responses of algae and invertebrates to a single application of N and P in a series of experimental wetland enclosures in the Interlake region of Manitoba during 1989 and 1990. N and P levels in the water, sediment and vegetation were also monitored. The 3 fertilization treatments were: dissolved inorganic (6200 µg 1–1 N, 420 µg 1–1 P), dissolved inorganic (3200 µg 1–1 N, 210 µg 1–1 P) and organic (ground alfalfa meal: 6200 µg 1–1 420 µg 1–1 P).Dissolved nutrients in the inorganic treatments were quickly depleted from the water column, but dissolved N increased in the water column of the alfalfa treatment as the alfalfa decomposed. No changes in N or P concentrations in the sediments or vegetation were detected. Phytoplankton biomass increased in all fertilized enclosures while epiphytic periphyton exhibited only minor responses. Epipelon biomass increased in the alfalfa treatment and metaphyton standing crops were highest in the high inorganic treatments.In the alfalfa treatment, high microbial respiration rapidly reduced dissolved oxygen concentrations which negatively affected invertebrates. This trend reversed as oxygen levels increased later in the experiment. Dominant nektonic and benthic invertebrates increased in the high inorganic and alfalfa treatments. Orthocladiinae emergence increased in the high inorganic and alfalfa treatments, while Chironominae and Tanypodinae increased in the alfalfa treatment. Second year responses by algae and invertebrate communities to the fertilization treatments were minimal. Annual single pulse fertilization has the potential to increase the productivity of Interlake wetlands when nutrients are applied in the spring, however it should be noted that at the levels used in this study the effects did not extend to the second year. 相似文献
15.
Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans 总被引:3,自引:0,他引:3
Susanne Grether-Beck Gabor L. Igloi Stefan Pust Emil Schilz Karl Decker Roderich Brandsch 《Molecular microbiology》1994,13(5):929-936
The genes of nicotine dehydrogenase (NDH) were identified, cloned and sequenced from the catabolic plasmid pA01 of Arthrobacter nicotinovorans. In immediate proximity to this gene cluster is the beginning of the 6-hydroxy-L-niotine oxidase (6-HLNO) gene. NDH is composed of three subunits (A, B and C) of Mr 30011, 14924 and 87677. It belongs to a family of bacterial hydroxylases with a similar subunit structure; they have molybdopterin dinucleotide, FAD and Fe-S clusters as cofactors. Here the first complete primary structure of a bacterial hydroxylase is provided. Sequence alignments of each of the NDH subunits show similarities to the sequences of eukaryotic xanthine dehydrogenase (XDH) but not to other known molybdenum-containing bacterial enzymes. Based on alignment with XDH it is inferred that the smallest subunit (NDHB) carries an iron-sulphur cluster, that the middle-sized subunit (NDHA) binds FAD, and that the largest NDH subunit (NDHC) corresponds to the molybdopterin-binding domain of XDH. Expression of both the ndh and the 6-hlno genes required the presence of nicotine and molybdenum in the culture medium. Tungsten inhibited enzyme activity but not the synthesis of the enzyme protein. The enzyme was found in A. nicotinovorans cells in a soluble form and in a membrane-associated form. In the presence of tungsten the fraction of membrane-associated NDH increased. 相似文献
16.
Presynaptic modulation by eicosanoids in cortical synaptosomes 总被引:1,自引:0,他引:1
In continuing experiments to determine the ionic basis of inhibitory presynaptic modulation, rat cortical synaptosomes were employed and receptor-activated K+ efflux was determined with a K+ sensitive electrode. When synaptosomes were sub-optimally depolarized by veratridine, the addition of agents that activated purinergic, 2, muscarinic and opioid receptors all promoted K+ efflux. With 2-chloroadenosine as a model inhibitory presynaptic modulator, the increased K+ efflux evoked by this agent was blocked by the cyclooxygenase inhibitor indomethacin suggesting that arachidonic acid or its metabolites was an intermediary in opening the channel. When arachidonic acid and PGE2 were tested, both promoted K+ efflux that was inhibited by dendrotoxin and mast cell degranulating peptide, two agents that are known to inhibit a delayed rectifier K+ current. Our results suggest that via eicosanoid second messengers, inhibitory presynaptic modulators open a sub-class of K channels that hyperpolarize nerve terminals, therefore less Ca2+ would enter per nerve impulse and thus the evoked release of neurotransmitters would be decreased.Abbreviations DTX
dendrotoxin
- MCDP
mast cell degranulating peptide
- NHGA
norhydroguairetic acid
- PGE2
prostaglandin E2 相似文献
17.
The kinetics of t-[3H]butylbicycloorthobenzoate (TBOB) binding to the convulsant sites of the γ-aminobutyric acidA (GABAA) receptor-ionophore complex were examined in synaptosomal membrane preparations of rat brain. On and off rates of TBOB binding were accelerated by 1 μM GABA and decelerated by 1 μM bicuculline methochloride, a GABAA antagonist. The presence of GABA and bicuculline methochloride created rapid and slow phases of dissociation, respectively. The three groups of rate constants distinguished for the dissociation of 4 nM and 30 nM [3H]TBOB represent multiaffinity states of the convulsant sites depending on the presence of GABA or bicuculline methochloride. Apparent association rate constants do not obey the equation kapp=koff±kon [TBOB] without assuming interconvertibility of the kinetic states during binding. Avermectin B1a (AVM B1a), a chloride channel opening agent, accelerated the association and dissociation of TBOB and resulted in a biphasic effect on TBOB binding, i.e., enhancement at low concentrations (EC50, 7.8 nM) followed by displacement at high concentrations (IC50 6.3 μM) of AVM B1a. AVM B1a resulted in similar biphasic effects on t- [35S]butylbicyclophosphorothionate binding. DIDS, an isothiocyanatostilbene derivative with irreversible anion channel blocking effect, selectively inhibited basal [3H]TBOB binding (IC50 125 μM DIDS) leaving the enhancement by AVM B1a unaffected. 相似文献
18.
Abstract Since 1990 under the Eastern Habitat Joint Venture over 100 small wetlands have been restored in Prince Edward Island, Canada. Wetlands were restored by means of dredging accumulated sediment from erosion to emulate pre‐disturbance conditions (i.e., open water and extended hydroperiod). In 1998 and 1999 we compared waterfowl pair and brood use on 22 restored and 24 reference wetlands. More pairs and broods of Ring‐necked Ducks, Gadwall, Green‐winged Teal, and American Black Ducks used restored versus reference wetlands. In restored wetlands waterfowl pair density and species richness were positively correlated with wetland/cattail area, percent cattail cover, and close proximity to freshwater rivers. In addition, a waterfowl reproductive index was positively correlated with percent cattail cover. Green‐winged Teal pair occurrence in restored wetlands was positively correlated with greater amounts of open water and water depths. American Black Duck pairs occurred on most (86%) restored wetlands. Restored small wetlands likely served as stopover points for American Black Duck broods during overland or stream movements, whereas they likely served as a final brood‐rearing destination for Green‐winged Teal broods. We suggest that wetland restoration is a good management tool for increasing populations of Green‐winged Teal and American Black Ducks in Prince Edward Island. 相似文献
19.
Uwe-G. Maier Stefan A. Rensing Gabor L. Igloi Martina Maerz 《Molecular & general genetics : MGG》1995,246(1):128-131
Introns within introns (twintrons) are known only from the Euglena chloroplast genome. Twintrons are group II or III introns, into which another group II or III intron has been transposed. In this paper we describe a non-Euglena twintron structure within a plastid-encoded chaperone gene (cpn60) of the cryptomonad alga Pyrenomonas salina. In addition, the evolutionary relationships between members of the Cpn60 protein family are determined. Our findings permit the inclusion of cryptomonad plastomes in phylogenetic studies of intron evolution and present further evidence for the origin of modern plastids from a cyanobacterial ancestor.This paper is dedicated to Prof. Dr. Peter Sitte on the occasion of his 65th birthday 相似文献
20.
Nathalie Doerflinger Catherine Linder Karim Ouahchi Gabor Gyapay Jean Weissenbach Denis Le Paslier Philippe Rigault Samir Belal Christiane Ben Hamida Faycal Hentati Mongi Ben Hamida Massimo Pandolfo Stephano DiDonato Ronald Sokol Herbert Kayden Pierre Landrieu Alexandra Durr Alexis Brice Fran?oise Goutières Alfried Kohlschütter Pascal Sabouraud Ali Benomar Mohamed Yahyaoui Jean-Louis Mandel Michel Koenig 《American journal of human genetics》1995,56(5):1116-1124
Ataxia with vitamin E deficiency (AVED) is an autosomal recessive disease characterized clinically by neurological symptoms with often striking resemblance to those of Friedreich ataxia. This disorder has been reported previously as familial isolated vitamin E deficiency. We have mapped recently the AVED locus to a 5-cM confidence interval on chromosome 8q by homozygosity mapping in six Mediterranean families. We have now analyzed six new and two previously described families and demonstrate genetic homogeneity despite important clinical variability and wide geographic origins. Analysis of nine new tightly linked microsatellite markers, including four characterized in this study, revealed a predominant but not unique mutation in northern African populations, where this condition is more frequent. Haplotype analysis but also classical recombinations allowed us to refine the AVED position to a 1-cM interval. A YAC contig over this interval was constructed from marker STSs and YAC fingerprint data, in order to facilitate the search of the AVED gene. 相似文献