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The goal of this project was the development of a serological test for the detection of Sclerotinia sclerotiorum on oilseed rape petals. Since the fungus exists in two forms on petals, as ascospores and mycelium, the responses of anti-mycelium and anti-ascospore antisera against these two kinds of antigens were compared. Two anti-mycelium sera, Smy and Smy', were produced against mycelial soluble extracts at different concentrations (0.3 mg and 0.1 mg of protein ml-1). Smy gave the greatest response level with eight S. sclerotiorum mycelial extracts tested. It had a very superior level of recognition to that of anti-ascospore serum, Ssp, when mycelium was tested as antigen. In contrast, Ssp and Smy were equally reactive when exposed to ascospores but the sensitivity of the assay was low. For each antiserum, the solution from which ascospores had been removed reacted similarly to the original suspension containing ascospores. A collection of fungi likely to be found on oilseed rape petals was examined. Cross-reactions were produced with both antisera, especially with Botrytis cinerea for which greater cross-reactivities were produced with Smy. The cross-absorption of antiserum Smy with a mycelial extract of B. cinerea considerably reduced this cross-reaction. The choice of antiserum for the development of a reliable detection system is discussed. 相似文献
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NEIL H. LANDMAN JEAN LOUIS DOMMERGUES DIDIER MARCHAND 《Lethaia: An International Journal of Palaeontology and Stratigraphy》1991,24(4):409-421
Several examples of progenetic species among Mesozoic ammonites are investigated. The ammonites are Middle Jurassic kosmoceratids, Upper Jurassic oppeliids, and Upper Cretaceous scaphites. As assessed through outgroup comparison, the progenetic species in each of these examples is characterized by small size at maturity and the presence of morphological features typical of those of the juveniles of more primitive species. However, in none of these examples is the progenetic species a duplicate form of the juvenile stage of more primitive species. There are at least three kinds of features of progenetic species that differ from those of the juveniles of more primitive species: (1) symplesiomorphous features associated with maturation, (2) unique features whose presence is not clearly related to progenetic processes, and (3) unique features that may represent epigenetic outgrowths of juvenile morphology. This novel combination of juvenile. adult. and unique features may endow progenetic species with the evolutionary potential to play a role in the origin of higher taxa. □ Progenesis, heterochrony, Ammon-oidea. Jurassic, Cretaceous. 相似文献
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Estimating total breeding populations (I) for species that exhibit biennial breeding is generally done from counts of individuals that breed in each year (N), but can be complicated by the fact that the proportion of individuals breeding varies from year to year. Partly, this reflects the proportion of individuals that re‐breed in successive years (re‐breeding rate, p), which is largely, although not exclusively, governed by reproductive failure. Here we show that variation in counts of breeding individuals not only reflects changes in total breeding population but can be sensitive to and powerfully driven by variation in p. A simulation of annual field counts of a bird exhibiting biennial breeding was constructed to explore the effect of re‐breeding attempts on estimations of the total breeding population. The model was used to simulate the consequences of adult mortality and different annual patterns of nesting failures on total breeding population estimates, and to explore the consequences of variation in p on N, when total breeding population remains constant. N is shown to be very sensitive to variations in p, so that even short‐term fluctuations in p can cause changes in N that oscillate for many years ahead. We compare our modelled results with real data for Grey‐headed Albatrosses Thalassarche chrysostoma and demonstrate that, when I is held constant in the model, actual counts may be simulated by variations in p only. Normally, I is unknown and is extrapolated from N on the assumption that N mirrors changes in the size of the total population. Consequently, applying average values of p can result in misleading estimates of total breeding population. We recommend that annual counts of breeding individuals are supplemented with annual estimates of p. Field protocols that aim to estimate annual breeding population size from counts of breeding individuals should be complemented by independent measures of rates of re‐breeding and nest failure. 相似文献
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Caroline Fraslon GaËlle Rolland Jacques R. Bourbon Michel Rieutort Cécile Valenza 《In vitro cellular & developmental biology. Animal》1991,27(11):843-852
Summary A serum-free culture medium (defined medium = DM) was elaborated by adding to Eagle’s minimum essential medium (MEM), non-essential
amino acids, transferrin, putrescine, tripeptide glycyl-histidyl-lysine, somatostatin, sodium selenite, ethanolamine, phosphoethanolamine,
sodium pyruvate, and metal trace elements. This medium was tested for its ability to support sustained surfactant biosynthesis
in fetal alveolar epithelial type II cells. For up to 8 days, ultrastructure was maintained with persistance of lamellar inclusion
bodies. Thymidine incorporation into DNA was enhanced about 50% in DM as compared with MEM, whereas it was enhanced 300% in
10% fetal bovine serum. With DM, the incorporation of tritiated choline into phosphatidylcholine (PC) of isolated surfactant
material was about twice that with MEM. Deletion experiments evidenced the prominent role of pyruvate, transferrin, and selenium
in the stimulation of surfactant PC biosynthesis. The addition of biotin to DM enhanced surfactant PC biosynthesis slightly
and nonsurfactant PC biosynthesis markedly. The presence of nucleosides seemed unfavorable to the synthesis of surfactant
PC. Type II cells responded to the addition of epidermal growth factor and insulinlike growth factor-I both by increased thymidine
incorporation into DNA and choline incorporation into PC. It is concluded that DM represents a useful tool for cultivating
type II cells without loss of their specialized properties and for studying the regulation of cell proliferation and surfactant
biosynthesis in a controlled environment. 相似文献
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Protein bodies, isolated by differential and isopycnic centrifugation, have been observed in transmission and scanning electron microscope and biochemically analysed. The powders of the axes and of the cotyledons contain numerous protein bodies, which in the scanning microscope appear to be surrounded by a more or less torn membrane. The proportion of intact, isolated protein bodies is influenced by the grinding methods, but even in the best conditions soaking disaggregates the majority of them. After isopycnic centrifugation, their debris gather in different density zones. Analyses of each zone have revealed that the caseinases are associated with particles of higher density than are peptidases and trypsin inhibitors. A minority population of small-size protein bodies resists the homogenization and fractioning modalities. A double origin of the protein bodies is considered. 相似文献
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SÉBASTIEN VILLÉGER GAËL GRENOUILLET VIRGINIE SUC SÉBASTIEN BROSSE 《Freshwater Biology》2012,57(11):2330-2341
1. We measured N and P excretion rates of 470 individuals belonging to 18 freshwater fish species widespread in Western Europe. We assessed the effect of body mass on excretion rates at both the intra‐ and interspecific levels. 2. The high variability in per capita N and P excretion rates was mainly determined by differences in body mass. The scaling coefficients of allometric relationships for both N and P excretion rates were significantly lower than 1 (mean ± SE, 0.95 ± 0.04 and 0.81 ± 0.05, respectively). 3. The slope of the allometric relationship between fish mass and nutrient excretion rate was significantly different among species. We did not detect any influence of phylogenetic conservatism on fish mass and on excretion rates. Further investigations are needed to understand the biological determinants of these differences. 4. This high intra‐ and interspecific variability in per capita excretion rates, coupled with differences in fish body mass, produce marked differences in biomass‐standardised excretion rates. These results thus indicate the necessity for further experimental and in situ investigations on the consequences of nutrient recycling by fish in freshwater ecosystems. 相似文献
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Variability in responses to thermal stress in parasitoids 总被引:1,自引:0,他引:1
GAËLLE AMICE PHILIPPE VERNON YANNICK OUTREMAN JACQUES VAN ALPHEN JOAN VAN BAAREN 《Ecological Entomology》2008,33(6):701-708
Abstract 1. To study phenotypic effects of stress, a stress is applied to cohorts of organisms with an increasing intensity. In the absence of mortality the response of traits will be a decreasing function of stress intensity because of increasing physiological costs. We call such decreasing functions type A responses. 2. However, when stress caused mortality, some studies have found that for high stress intensities, survivors performed as well as control individuals (type B responses). We proposed that type A responses are caused by the physiological cost of stress whereas type B responses are caused by a mixture of physiological costs and selection. 3. The present study exposed Aphidius picipes wasps to an increasing duration of cold storage (cold stress), and obtained variable responses as predicted when both physiological costs and selection of resistant individuals determine the outcome. 4. When cold storage of parasitoids for biological control is desirable, research should be carried out to find (i) the temperature regime and duration of storage and (ii) the least sensitive stage for storage to minimise losses from mortality and reduction of fitness of survivors. 5. Selection by cold stress as observed in the present study could result in rapid adaptation of populations exposed to such stress. 相似文献