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21.
Synthesis of Cell Coat in Normal and Transformed Cells 总被引:6,自引:0,他引:6
THE surface of transformed cells has been a focus of considerable attention recently because some of the properties which distinguish these cells from their precursors, such as decreased cell adhesiveness, altered cell orientation and loss of contact and density dependent inhibition1–3, may relate to changes on their surface. A common feature of vertebrate cells is the cell coat, a glycoprotein structure surrounding the plasma membrane4. Electron microscopy has revealed that transformed cells have a thicker coat than normal cells5 and we have now found that coat synthesis in cells transformed by an oncogenic DNA virus and in cells transformed by a chemical carcinogen occurs faster than in normal controls whereas only in the virus-transformed cells is the coat significantly thicker. 相似文献
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CLYDE M. SENGER DATUS M. HAMMOND JOSEPH L. THORNE A. EARL JOHNSON G. MARK WELLS 《The Journal of eukaryotic microbiology》1959,6(1):51-58
SYNOPSIS. An immunity to reinfection with E. bovis was demonstrated in 3 experiments involving 60 calves. This immunity develops rapidly, as indicated by resistance to a challenge given 14 days after the immunizing inoculation. In 3 groups of 3 to 6 young calves each, immunity was still present to a moderate degree 2 to 3 months after inoculation; in one group of 5 animals about a year old there was apparently a high degree of immunity about 7 months after the last inoculation. In one experiment an immunizing inoculum of 10,000 oöcysts did not produce as much immunity as 50,000 oöcysts. In 2 experiments there appeared to be little difference in the immunity produced by a single inoculation of 50,000 as compared with 100,000 oöcysts, but inoculation with 100,000 oöcysts, resulted in substantially longer and more severe illness than 50,000 oöcysts. There appeared to be no appreciable difference in clinical symptoms or development of immunity between calves given a single immunizing inoculum and those given the same number of oöcysts in 5 equal inocula on successive days. Treatment with sulfamethazine and sulfamerazine (Merameth) 13 to 15 days after inoculation alleviated the clinical symptoms of coccidiosis without interfering appreciably with the development of immunity. In one experiment with 7 calves, no beneficial effect was noted from 1 or 2 transfusions of 500 ml. of plasma and leucocytes from immune calves into 4 calves 1 and 12 days or 11 days after a challenge inoculation. 相似文献
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KATHARINE J. M. DICKINSON DAVE KELLY ALAN F. MARK GUDRUN WELLS RICHARD CLAYTON 《Austral ecology》2007,32(2):155-168
Abstract Establishing what are the underlying causes of species range limits is of fundamental interest in ecology. We followed the fate of individually mapped plants of three endemic New Zealand high‐alpine species of Myosotis, over a period of 9 years. The species provide contrasts in their geographical range and their demography. Myosotis oreophila Petrie is rare and extremely localized (c. 0.5 ha extent), Myosotis cheesemanii Petrie is regionally endemic, and Myosotis pulvinaris Hook. f. is more widespread. All three occur on the Dunstan Mountains, Central Otago, South Island, New Zealand within a 1‐km radius, and individual plants were followed in four permanent plots. The three species differed in their longevity and in population variability, with the most widespread species (M. pulvinaris) having the lowest survival (61% per year), the fewest old plants (only 3% of plants present in 1993 surviving until 2001), no increase in survival rates with age, and the most variability in total numbers across years. Both of the rare species, M. oreophila and M. cheesemanii, had higher survival (75% and 88%, respectively, per year) especially for older plants, many older plants (20% and 59%, respectively, of 1993 plants surviving until 2001), and lower variability in total numbers across years. These results are consistent with other studies showing that rare plant species tend to have higher inertia than more common congeners. The range limits of M. oreophila showed a high level of spatial constancy on a scale of metres over the 9 years, despite 80% turnover in plants during that time. The M. oreophila population showed lower mean densities of plants near to the population boundaries, identical age‐specific survival rates, but lower flowering probabilities, than the core of the population. We were unable to detect any abiotic differences between inside and outside the M. oreophila range in terms of topography, soil parent materials, microclimate or through manipulation of snow cover. Disturbance may be a factor affecting the distribution of M. cheesemanii but limiting factors for M. oreophila and M. pulvinaris are likely to be biotic (competition, seed limitation, dispersal capacity) and/or historic. Further experimentation is recommended. 相似文献