Differential Expression of microRNAs and their Regulatory Networks in Skin Tissue of Liaoning Cashmere Goat during Hair Follicle Cycles

MicroRNAs (miRNAs) are endogenous small noncoding RNA molecules that negatively regulate gene expression. Herein, we investigated a selective number of miRNAs for their expression in skin tissue of Liaoning Cashmere goat during hair follicle cycles, and their intracellular regulatory networks were constructed based on bioinformatics analysis. The relative expression of six miRNAs (mir-103-3p, -15b-5p, 17-5p, -200b, -25-3p, and -30c-5p) at anagen phase is significantly higher than that at catagen and/or telogen phases. In comparison to anagen, the relative expression of seven miRNAs (mir-148a-3p, -199a-3p, -199a-5p, -24-3p, -30a-5p, -30e-5p, and -29a-3p) was revealed to be significantly up-regulated at catagen and/or telogen stages. The network analyses of miRNAs indicated those miRNAs investigated might be directly or indirectly involved in several signaling pathways through their target genes. These results provided a foundation for further insight into the roles of these miRNAs in skin tissue of Liaoning Cashmere goat during hair follicle cycles.     

β2糖蛋白I及其抗体在抗磷脂抗体综合症病理进程中的作用探讨

抗磷脂抗体综合症(Antiphospholipid syndrome,APS)是以反复的动脉和静脉血栓形成为特征,与内皮细胞及单核淋巴细胞功能失调有关。β2糖蛋白Ⅰ是一种磷脂结合型糖蛋白,抗β2糖蛋白Ⅰ抗体与血栓形成密切相关,并且在APS的发生发展过程中具有决定性作用,但是抗β2糖蛋白Ⅰ及其抗体的功能尚未阐明。本文将围绕β2GPⅠ及其抗体的结构、生物学功能及其在血栓形成中的作用等方面进行详细的阐述,期望为APS发生发展过程中抗原抗体复合物调节细胞功能的分子机制提供新的视点。     

Molecular Characterization and Expression Analysis of ftr01, ftr42, and ftr58 in Zebrafish (Danio rerio)

Tripartite motif(TRIM) proteins were shown to play an important role in innate antiviral immunity. FinTRIM(ftr) is a new subset of TRIM genes that do not possess obvious orthologs in higher vertebrates. However, little is known about its function. In this study, we used bioinformatic analysis to examine the phylogenetic relationships and conserved domains of zebrafish(Danio rerio) ftr01, ftr42, and ftr58, as well as qualitative real-time PCR to examine their expression patterns in zebrafish embryonic fibroblast(ZF4) cells and zebrafish tissues. Sequence analysis showed that the three finTRIMs are highly conserved, and all contain a RING domain, B-box domain, and SPRY-PRY domain. In addition, ftr42 and ftr58 had one coiled-coil domain(CCD), whereas ftr01 had two CCDs. Tissue expression analysis revealed that the m RNA level of ftr01 was the highest in the liver, whereas those of ftr42 and ftr58 were the highest in the gill; the expression of thesefinTRIMs was clearly upregulated not in the eyes, but in the liver, spleen, kidney, gill, and brain of zebrafish following spring viremia of carp virus(SVCV) infection. Similarly, the expression of these three finTRIM genes also increased in ZF4 cells after SVCV infection. Our study revealed that ftr01, ftr42, and ftr58 may play an important role in antiviral immune responses, and these findings validate the need for more in-depth research on the finTRIM family in the future.     

林分因子对云顶山不同人工林林下植物多样性的影响

该研究采用典型抽样法,以成都云顶山5种人工林——柏木 枫杨林(BF)、银杏 楠木林(YN)、光皮梾木 香樟林(GZ)、枫杨 桤木林(FQ)、柏木林(CB)为研究对象,分析不同人工林的林下植物组成与多样性特征,并确定影响林下植物多样性的主导林分因子,为当地人工林经营管理提供理论依据。结果表明：(1)研究区共记录林下植物168种,隶属于62科130属;5种人工林灌木层与草本层的科属种数均以GZ最多。(2)5种不同人工林灌木层与草本层的优势种数分别为7、4、7、6、4种和5、4、9、9、10种,数量都较少。(3)5种人工林的Shannon Wiener多样性指数(H)、Simpson优势度指数(H′)、物种丰富度指数(D)、Pielou均匀度指数(J_sw) 均基本表现为草本层>灌木层,BF、GZ灌木层的D略高;灌木层的H、H′、D值均以GZ最大,但不同人工林的J_sw差异不显著;草本层的H、H′、D、J_sw均基本呈现CB>FQ>GZ>BF>YN趋势,GZ的D值略高于FQ。(4)6个林分因子对灌木层4个物种多样性指数的影响均无显著差异;林分平均树高、平均枝下高、平均胸径、平均冠幅和林分密度是影响草本层H、D的主要因子,但各林分因子对草本层H′、J_sw的影响差异不显著。研究认为,林分结构对林下草本层物种多样性的影响更大,平均树高、平均枝下高、平均胸径、平均冠幅、林分密度对草本层多样性有显著影响。     

EGFR配体生物学效应的多样性

表皮生长因子受体（epidermal growth factor receptor, EGFR）的配体作为一类重要的信号分子参与了细胞功能的调节,并且和机体发育、器官形成、组织修复与稳态维持,以及疾病的发生密切相关。虽然这些信号分子具有序列和结构上的相似性,但由于这些信号分子结构上的细微差异以及它们受体信号传导上的复杂性,造成了这些信号分子（配体）生物学效应的多样性。目前,从结构和机制上,对于单个信号分子的生物学效应已有较为深入的研究,但这些信号分子之间以及信号分子与受体之间的调控网络较为复杂,并且这种调控网络对信号的精细、有序和多样化转导至关重要。本文对EGFR配体的结构及配体生物学效应多样性的分子机制进行回顾,并对未来的研究方向提出展望。     

与医学相关的营养与代谢方面的新概念

近年来,与医学相关的营养与代谢的研究出现不少新的认识与新发现。本文总结了以下5个方面的新概念：供能的营养物质不仅是供能,还需注意他们对健康的影响;以构成蔗糖分子一半的果糖为例,它竟然是促成代谢综合征、心脑血管病等疾病的因素;食物纤维是极其重要不可或缺的营养素;应高度重视维护正常肠道菌群,因为它与人体的健康与多种疾病密切相关;全食物营养不仅能维持生命,还能增强体质、避免和减少疾病;植物营养素具有抗氧化、抗炎症及抗癌等作用,是全食物营养不可或缺的组成部分。人体营养与物质代谢研究的终极目标是：增强体质,预防和减少疾病,健康长寿。希望本文能够带给读者一些启发。     

Probiotic Therapy (BIO-THREE) Mitigates Intestinal Microbial Imbalance and Intestinal Damage Caused by Oxaliplatin

Probiotics and Antimicrobial Proteins - Gastrointestinal mucositis associated with the use of chemotherapeutic drugs can seriously affect the quality of life of patients. In this study, a probiotic...     

细胞因子在ARDS发病机制中的作用

细胞因子是由多种细胞产生的多肽或低分子糖蛋白,在人体内含量极微,在pg水平就发挥作用。作为特异性免疫反应和非特异性免疫反应的蛋白质,细胞因子以自分泌、旁分泌、或内分泌方式产生,与相应的细胞表面受体结合,在局部或全身发挥复杂的生物学效应,它们的代谢异常和疾病的发生、发展有着密切的关系。有些细胞因子已应用于临床的生物学治疗,具有深远的临床应用价值,故对细胞因子的研究将是一个越来越重要的课题。急性肺损伤(ALI)/急性呼吸窘迫综合征(ARDS)发病机制错综复杂,大量临床和实验室研究证明多种效应细胞释放的炎症介质是造成ARDS的"中心环节",其中TNF-α、IL-1、IL-8、IL-10、CXC趋化因子等细胞因子在ARDS发病中的作用尤为重要。本文就细胞因子在ARDS发病机制中的作用做一综述。     

Correlation between haplotype of apolipoprotein B gene and natural longevity persons in Uygur Nationality

This paper investigated the correlation between polymorphisms and haplotypes in the apolipoprotein B (apoB) gene (SP-I/D, XbaI-RFLP, VNTR) and natural longevity persons among the Uygur people in Xin-jiang. For this purpose, 191 healthy Uygur individuals aged above 90 from Hetian area of Xinjiang were recruited, and another 53 persons aged 65—70 from the same nationality, the same region and with the same gender ratio, served as the control group. Genotyping was performed by PCR-SSP, PCR-RFLP and PCR-sequencing methods. Logistic regression analyses revealed that the frequencies of X X genotype, M and L alleles and the genetypes composed of M and L were significantly higher in the longevity group than in the control group. In haplotype analyses, we found that, in the long-lived people, the frequency of haplotypes composed of the X and M alleles was significantly higher whereas the frequency of haplotypes composed of the X- and S alleles was significantly lower (both P<0.05) I than those of their controls. These results indicated that the S allele, SS genotype and X -S, D-S, D-X -S haplotypes were the possible adverse factors, whereas the M, L alleles, X X , MM, ML, LL genotypes and I-X -M, X -M haplotypes were the possibe protective factors for the naturally long-lived Uygur people in China.     

哺乳期需氧菌性阴道炎患者定期补充乳杆菌的疗效

目的分析在有效抗炎基础上定期补充乳杆菌活菌胶囊对哺乳期需氧菌性阴道炎患者的疗效及对远期复发率的影响。方法选择2019年4月至2020年3月于大连市妇女儿童医疗中心(集团)行产后42 d检查时发现的需氧菌性阴道炎患者共120例,按照随机自愿原则将所选患者分为观察组及对照组,各60例。全部患者均使用红核妇洁洗液及克林霉素磷酸酯阴道凝胶抗炎治疗7 d,观察组患者于第8天开始给予阴道用乳杆菌活菌胶囊每日睡前上药1粒,治疗10 d,停药后1周对两组患者进行临床疗效评估并了解不良反应发生情况。于首次治疗结束后第3个月及第6个月进行定期随诊,了解两组患者阴道炎的复发情况。且观察组患者于治疗后第3个月随诊结束后再次给予阴道用乳杆菌活菌胶囊补充上药1疗程。记录并比较两组患者治疗前、治疗后1周、治疗后第3个月及第6个月阴道pH值及H₂O₂情况。结果两组患者治疗期间均未发生明显不良反应。治疗后1周观察组患者治愈率(66.7%)显著高于对照组(36.7%),差异有统计学意义(P<0.05);观察组总有效率(95.0%)与对照组(86.7%)比较差异无统计学意义...