Molecular chaperone HspB2 inhibited pancreatic cancer cell proliferation via activating p53 downstream gene RPRM,BAI1, and TSAP6

Heat shock proteins (HSPs) were known as the molecular chaperones, which play a pivotal role in the protein quality control system, ensuring correct folding of proteins, and facilitating the correct refolding of damaged proteins via the transient interaction with their substrate proteins. They also practice in the regulation of cell cycles and are involved in apoptosis. We found that HspB2 was almost completely silent in pancreatic cancer and few studies investigated the role of HspB2 in cancer cells, particularly in pancreatic cancer. Here, we reported that HspB2 effectively inhibited cell proliferation in Panc-1 cells. Specifically, we demonstrated that HspB2 could combine mut-p53 and change the DNA binding site of mutant p53, subsequently upregulated the expression of RPRM, BAI-1, and TSAP6 which were the downstream genes of wt-p53, participate in mediating downstream responses to p53, including inhibiting cell proliferation and angiogenesis. The main aim of this study is to investigate the relationship between HspB2 and p53, and provide a novel treatment strategy for pancreatic cancer.     

A risk signature-based on metastasis-associated genes to predict survival of patients with osteosarcoma

Osteosarcoma (OS) is the most common primary solid malignant bone tumor, and its metastasis is a prominent cause of high mortality in patients. In this study, a prognosis risk signature was constructed based on metastasis-associated genes. Four microarrays datasets with clinical information were downloaded from Gene Expression Omnibus, and 256 metastasis-associated genes were identified by limma package. Further, a protein-protein interaction network was constructed, and survival analysis was performed using data from the Therapeutically Applicable Research to Generate Effective Treatments data matrix, identifying 19 genes correlated with prognosis. Six genes were selected by the least absolute shrinkage and selection operator regression for multivariate cox analysis. Finally, a three-gene (MYC, CPE, and LY86) risk signature was constructed, and datasets GSE21257 and GSE16091 were used to validate the prediction efficiency of the signature. The survival times of low- and high-risk groups were significantly different in the training set and validation set. Additionally, gene set enrichment analysis revealed that the genes in the signature may affect the cell cycle, gap junctions, and interleukin-6 production. Therefore, the three-gene survival risk signature could potentially predict the prognosis of patients with OS. Further, proteins encoded by CPE and LY86 may provide novel insights into the prediction of OS prognosis and therapeutic targets.     

实时荧光定量PCR及RT-PCR与常规PCR及RT-PCR检测对虾病毒效果的比较

常规PCR及RT-PCR已用于对虾DNA及RNA病毒检测,但存在费时、灵敏度较低、不能定量等问题。建立了TaqMan实时荧光定量PCR及RT-PCR方法,分别用于检测白斑综合症病毒(WSSV)、传染性皮下及造血组织坏死病毒(IHHNV)及桃拉综合征病毒(TSV)、黄头病毒(YHV)4种对虾病毒。与常规PCR及RT-PCR比较,所建立的TaqMan实时荧光定量PCR及RT-PCR检测上述4种对虾病毒不仅有很高的特异性,检测灵敏度也提高了10～100倍,同时还具有快速、简便、不污染环境、重复性好、实时定量等优点,可明显提高对虾病毒检验检疫工作质量及效率。     

电子载体对丁醇发酵的影响

研究在培养基中加入不同电子载体对丁醇发酵的影响。结果表明:添加微量的苄基紫精可以促进丁醇的产生,同时可强烈抑制丙酮的合成,丁醇体积分数由66.92%提高到82.35%。苄基紫精可促进菌株快速进入产溶剂期,发酵周期明显缩短,丁醇生产强度显著提高。7%玉米培养基中加入40 mg/L苄基紫精,丁醇产量最高达16.10 g/L,生产强度为0.37 g/(L.h),分别较对照提高10.96%和60.87%。在初始丁醇体积分数较低的条件下,苄基紫精对丁醇合成的促进作用更明显。     

蛋白质O-GlcNAc修饰的检测技术

O-连接的N-乙酰葡糖胺（O-GlcNAc）修饰是位于细胞浆和细胞核蛋白质的丝氨酸或苏氨酸上的一种翻译后修饰,在高等真核生物细胞中广泛存在.越来越多的研究表明,O-GlcNAc修饰在代谢调控、压力应激、细胞周期、凋亡、糖尿病、心血管疾病和癌症等多种生理和病理过程中发挥重要作用,因此, O-GlcNAc修饰已受到众多生命科学领域研究人员的关注.然而,由于O-GlcNAc修饰与传统的N聚糖和O聚糖修饰有所不同,常规糖基化修饰的检测方法并不适用于O-GlcNAc.本文对O-GlcNAc修饰的检测及其修饰位点的确定方法进行了综述,并分析了各种方法的优缺点.     

山梨酸钾和D-异抗坏血酸钠联合作用HepG2细胞的生物学效应分析

该文研究食品添加剂联合作用人肝癌HepG2细胞后的多参数生物学指标,并探索可能存在的损伤机制。CCK-8法检测受试物山梨酸钾及D 异抗坏血酸钠0.13～ 2.00 g/L分别作用HepG2细胞24、48及72 h后的细胞增殖活力,显示山梨酸钾及D-异抗坏血酸钠均呈显著的时间、剂量依赖性的抑制HepG2细胞增殖,其24 h的IC50分别为1.35±0.11 g/L和1.58±0.17 g/L。高内涵分析结果表明,与单独组相比,联合组显著降低细胞数量和线粒体膜电位,增大细胞膜通透性及活性氧水平(P＜0.05);高剂量联合组(0.30+0.41 g/L)显著增加DNA损伤(P＜0.01),表现为协同作用。qRT-PCR和Western印迹法显示,山梨酸钾及D 异抗坏血酸钠可显著提高P53、Caspase 3、Bax、γ-H2AX表达,同时显著降低Bcl-2表达,从而抑制HepG2细胞增殖。     

铅对大鼠海马神经细胞H-电流特性的影响

应用全细胞膜片钳技术研究了铅对大鼠海马神经细胞H－电流特性的影响。结果表明：铅抑制了H－电流表达;降低了H－通道电压敏感性和升高了H－通道的激活电压。铅对H－电流的压抑效应有其对H－通道电压敏感性的改变,是铅损伤神经活动的一个因素之一。     

反义hTR抑制人胰腺癌P3细胞系端粒酶活性和增殖的研究

设计针对端粒酶RNA模板序列并经硫代磷酸修饰的正义、反义和随机序列寡核苷酸,以正常人成纤维细胞作对照,观察其对人胰腺癌细胞端粒酶活性和细胞生长增殖的影响作用以及对正常细胞是否有毒副作用。结果表明：针对hTR模板区的反义寡核苷酸能降低胰腺癌P3细胞端粒酶活性,抑制细胞生长,促进细胞周期改变并诱导细胞发生凋亡,而且对正常细胞没有明显的毒副作用。因此我们认为利用反义技术封闭hTR基因很可能成为治疗肿瘤的安全、有效手段之一。 Abstract:This paper is to investigate PS-ODN's (antisense-PS-ODN of hTR,sense-PS-ODN of hTR and random sequence) effects on telomerase activity and proliferation of P3 pancreatic cancer cells,and to find a novel method for gene therapy of pancreatic cancer.The results indicate that the anti-hTR complementary to the template region of hTR is sufficient to inhibit P3 cell telomerase activity and cell proliferation in vitro,and as a result,they can lead to a profound induction of programmed cell death.Telomerase represents an interesting and promising anticancer drug target and antitelomerase technology may have potential significance in tumor therapy.     

繁缕和无瓣繁缕六个居群的数值分析

对繁缕（Stellaria media）和无瓣繁缕（S.apetala）的6个居群的57个性状进行Q－聚类和R－聚类的研究。结果表明：（1）Q－聚类中,用一条结合线,可以把繁缕的4个居群聚为一类,无瓣繁缕的2个居群聚为一类。这一结果支持肥繁缕和无瓣繁缕划分为两个物种;（2）R－聚类中,发现了呈现完全正相关、极大正相关和极大负相关的性状,并根据R－聚类的结果,运用一条适当的结合线,把繁缕和无瓣繁缕的57个性状划为5个类群,并分析了各性状的分类学意义。