Evaluation of UV-Induced Mutants of Chlorella vulgaris for Single Cell Protein I. The Screening Effect of Cycloheximide and 6-Methylpurine

Chlorella vulgaris was irradiated with UV doses allowing a 0.1 per cent survival. Dark recovery and photoreactivation were carried out either in the presence or in the absence of an inhibitor of protein synthesis. Faster growing and faster greening colonies were selected, and the amino acid composition of the mutant strains were evaluated. Higher growth and photosynthetic rates, higher chlorophyll content and lower respiration rate were shown by all mutants irrespective of the selection procedure. Selection against cycloheximide led to higher protein and RNA, but lower carotenoid content, whereas mutants selected against 6-methylpurine showed, in addition to a protein and carotenoid increase, a more favourable protein: RNA ratio.     

SOME SOURCES OF BIAS IN CONSUMER TESTING

Data sets from three studies were examined to determine the effects of brand identification and package design on consumer responses during product evaluation. In these studies, consumers evaluated products with or without brand identification, compared products in standardized conditions, and compared products in different package designs with different or identical product contents. Measurement of consumer responses for all studies was based on 9-point hedonic scale. The results of the study showed that consumers were less critical in their evaluation when the samples being assessed were identified by brand names. Brand identification and package design, when not concealed in the comparison of product contents, resulted in consumer response bias. Also, the popularity of a brand influenced consumer's perception of products.     

TRUSTED CONSENT AND RESEARCH BIOBANKS: TOWARDS A ‘NEW ALLIANCE’ BETWEEN RESEARCHERS AND DONORS

We argue that, in the case of research biobanks, there is a need to replace the currently used informed consent with trusted consent. Accordingly, we introduce a proposal for the structure of the latter. Further, we discuss some of the issues that can be addressed effectively through our proposal. In particular, we illustrate: i) which research should be authorized by donors; ii) how to regulate access to information; iii) the fundamental role played by a Third Party Authority in assuring compliance with the reciprocal expectations and obligations of donors and scientists. Finally, we briefly analyse two issues that might represent important elements of a ‘new alliance’ between researchers and donors to which the trusted consent could pave the way: i) the correlations between needs and rights of the two parties, and ii) possible economic transactions.     

Serological relationships among Sophoreae, Thermopsideae and Genisteae (Fabaceae)

A comparison among 20 genera, representatives of Sophoreae, Thermopsideae and Genisteae based on the serological cross reactivity of the seed proteins, indicates: (a) Sophoreae are highly heterogeneous – there is little similarity among genera of the New World, genera of tropical austral Africa, and genera of temperate Eurasia; (b) Sophora and Maackia are strictly related both to Thermopsidae and to Genisteae; (c) Thermopsideae are not intermediate between Sophoreae and Genisteae; (d) Primitive Thermopsideae and Genisteae (Anagyris, Hesperolaburnum) are serologically related among themselves and to the Sophoreae; (e) derived herbaceous genera (Baptisia, Lupinus) are serologically divergent.     

RELATIONSHIPS BETWEEN PATELLA FERRUGINEA GMELIN, 1791 AND THE OTHER TYRRHENIAN SPECIES OF PATELLA (GASTROPODA: PATELLIDAE)

The external morphology of soft parts of the rare Patella ferrugineaGmelin, 1791 is described, and its relationships with P. caerulea,P. rustica and P. ulys-siponensis are investigated on both morphologicaland electrophoretic grounds. Soft part morphology is a gooddiscriminative tool, but of little help in detecting affinities.Electrophoretic data on 19 presumptive loci suggest that P.rustica is the closest species to P. ferruginea, whereas P.caerulea and P. ulyssiponensis are clustered separately. Meanobserved heterozyg-osities arc higher in P. ferruginea and P.rustica. It is concluded that in case of subdivision of thegenus Patella L., 1758, P. ferruginea and P. rustica shouldbe placed together in the subgenus Patellastra Mon-terosato,1884. (Received 6 January 1993; accepted 23 February 1993)     

Monomorphic Region of the Serotonin Transporter Promoter Gene in New World Monkeys

Genetic variation in the human serotonin system has long been studied because of its functional consequences and links to various neuropsychiatric and behavior‐related disorders. Among non‐human primates, the common marmosets (Callithrix jacchus) and tufted capuchins monkeys (Cebus apella) are becoming increasingly used as models to study the effects of genes, environments, and their interaction on physiology and complex behavior. In order to investigate the independent functions of and potential interactions between serotonin‐related genes, anxiety and neuropsychiatric disorders, we analyzed the presence and variability of the serotonin transporter gene‐linked polymorphic region (5‐HTTLPR) in marmoset and capuchin monkeys. By PCR and using heterologous primers from the human sequence, we amplified and then sequenced the corresponding 5‐HTT region in marmosets and capuchins. The resulting data revealed the presence of a tandem repeat sequence similar to that described in humans, but unlike humans and other Old World primates, no variable length alleles were detected in these New World monkeys, suggesting that if serotonin transporter is involved in modulating behavior in these animals it does so through different molecular mechanisms. Am. J. Primatol. 74:1028‐1034, 2012. © 2012 Wiley Periodicals, Inc.     

Killing in the Pliocene: shark attack on a dolphin from Italy

Abstract: Shark bite marks, including striae, sulci and abrasions, in a well‐preserved fossil dolphin skeleton referred to Astadelphis gastaldii (Cetacea, Delphinidae) from Pliocene sediments of Piedmont (northern Italy), are described in detail. The exceptional combination of a fossil dolphin having a significant part of the skeleton preserved and a large number of bite marks on the bones represents one of the few detailed documentations of shark attack in the past. Most bite marks have been referred to a shark about 4 m long with unserrated teeth, belonging to Cosmopolitodus hastalis, on the basis of their shape and their general disposition on the dolphin skeleton. According to our hypothesis, the shark attacked the dolphin with an initial mortal bite to the abdomen from the rear and right, in a similar way as observed for the living white shark when attacking pinnipeds. A second, less strong, bite was given on the dorsal area when the dolphin, mortally injured, probably rolled to the left. The shark probably released the prey, dead or dying, and other sharks or fishes probably scavenged the torn body of the dolphin.