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GIOVANNI BERNARDINI ANGELA M. RIZZO ROSALBA GORNATI FEDERICA ROSSI BRUNO BERRA 《Cell biology international》1996,20(10):667-672
Xenopus embryos contain a considerable amount of a polysialo-ganglioside not yet fully characterized; in this paper, we will refer to it as ganglioside X1. Preliminary experiments indicate asialo-GM1 as the core structure of the ganglioside X1 and palmitic and oleic acid as the fatty acids of the ceramide moiety. Further analyses by comparative 2D-TLC with adult fish and chick embryo brains indicate the pentasialilated ganglioside GP1c as the possible structure of X1. In the adult Xenopus, X1 characterizes the ganglioside pattern of the central nervous system while is absent in all the other tested tissues. At least two other more polar (presumably richer in sialic acid) bands are often visible under X1, both in embryos and in brain and spinal cord tissues of adult Xenopus. The persistence of polysialo-gangliosides in the brain and spinal cord of adult amphibians could serve to guarantee a proper functioning of the central nervous system at low body temperature. 相似文献
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Killer sperm whale: a new basal physeteroid (Mammalia, Cetacea) from the Late Miocene of Italy 总被引:1,自引:0,他引:1
Zygophyseter varolai , a new genus and species of Physeteroidea (Cetacea, Odontoceti), is based on an almost complete skeleton from the Late Miocene (Tortonian) in southern Italy. The extreme elongation of the zygomatic process of the squamosal and the circular supracranial basin (probably for housing the spermaceti organ) delimited by a peculiar anterior projection of the supraorbital process of the right maxilla are the most distinctive features of this bizarre sperm whale. Large body size, large teeth present in both lower and upper jaw, and anteroposteriorly elongated temporal fossa and zygomatic process of the squamosal indicate that this cetacean (for which we suggest the English common name killer sperm whale) was an active predator adapted to feeding on large prey, similarly to the extant killer whale ( Orcinus orca ). A phylogenetic analysis reveals that Zygophyseter belongs to a Middle–Late Miocene clade of basal physeteroids, together with Naganocetus (new genus for the type of ' Scaldicetus ' shigensis ). Moreover, the phylogenetic analysis shows evidence of a wide physeteroid radiation during the Miocene and that the extant Physeter and Kogia belong to two distinct families that form a clade representing the crown-group Physeteroidea. © 2006 The Linnean Society of London, Zoological Journal of the Linnean Society , 2006, 148 , 103–131. 相似文献
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RICARDO T. GAZZINELLI ALVARO J. ROMANHA GILBERTO FONTES EGLER CHIARI GIOVANNI GAZZINELLI ZIGMAN BRENER 《The Journal of eukaryotic microbiology》1991,38(4):320-325
We observed a wide distribution of the carbohydrate epitopes galactosylα(1–3) galactose (galα1–3 gal), α-glucoside, and α-mannoside in mono- and heteroxenic trypanosomatids by using fluorescein-labelled lectins of Euonymus europaeus (EE) and Concanavalin A (Con A) as well as sera from acute chagasic patients who have very high levels of anti-galα(1–3) gal antibodies. The direct fluorescence test for galα1–3 gal with EE was positive at minimum concentrations of 6 μg/ml for heteroxenic trypanosomatids and 0.7 μg/ml for monoxenic ones and for the plant parasite, Phytomonas. On the other hand, heteroxenic trypanosomatids that infect vertebrates bound ten-fold more Con A than monoxenic flagellates and Phytomonas. These data were confirmed in ELISA and Western Blot assays carried out with peroxidase-labelled EE and Con A. Euonymus europaeus recognized several glycoproteins in all trypanosomatids that we tested. Con A, however, recognized a glycoprotein cluster in heteroxenic protozoa, which ranging from 60–120 kDa, seemed to lack monoxenic parasites and Phytomonas. These findings suggest that α-D-mannose and α-D-glucose might play an important role in the interaction between trypanosomatids and vertebrate hosts. 相似文献