汉黄芩素通过调控上皮间质转化抑制胃癌细胞的侵袭转移

目的:汉黄芩素是中药黄芩中的一种黄酮,具有体内外抗癌活性。然而,汉黄芩素对人胃癌细胞的作用尚不十分清楚。本研究拟探讨汉黄芩素对人胃癌细胞MGC-803侵袭转移能力的影响及其对上皮间质转化(Epithelial to Mesenchymal Transition,EMT)的作用机制。方法:采用MTT法测定汉黄芩素对人胃癌细胞MGC-803增殖能力的影响,通过划痕实验、Transwell试验检测汉黄芩素对人胃癌细胞MGC-803迁移、侵袭能力的影响。通过免疫印迹法和免疫荧光法分析汉黄芩素对EMT的影响。结果:20μM以上浓度的汉黄芩素能抑制人胃癌细胞MGC-803的增殖,不同浓度的汉黄芩素能抑制人胃癌细胞MGC-803的迁移和侵袭,且呈浓度依赖性。此外,汉黄芩素能抑制间质标记蛋白波形蛋白(Vimentin)和锌指蛋白E-盒结合同源异形盒-1(ZEB1)的表达,促进上皮标记蛋白E-钙黏蛋白(E-cadherin)的表达。结论:汉黄芩素能抑制胃癌细胞的侵袭和迁移,这一作用可能与其抑制EMT的发生有关。     

幽门螺杆菌感染性胃癌组织中cyclinD1、MMP-9的表达及其临床意义

目的:探讨幽门螺杆菌(HP)感染性胃癌组织中细胞周期蛋白D1(cyclinD1)、基质金属蛋白酶-9(MMP-9)的表达及其临床意义。方法:选取2016年12月到2018年6月期间在兰州大学第一医院接受治疗的胃癌患者80例,收集其手术切除的病理组织。采用C-14呼气试验和改良Giemsa染色检测患者HP感染的情况,采用免疫组化法检测胃癌组织中cyclinD1、MMP-9表达情况。分析HP感染、cyclinD1、MMP-9表达与胃癌患者临床病理特征的关系,并分析胃癌患者HP感染与cyclinD1、MMP-9表达的相关性。结果:80例胃癌患者HP感染阳性56例(70.00%),阴性24例(30.00%)。有淋巴结转移、浸润深度为T3+T4的胃癌患者的HP感染阳性率高于无淋巴结转移、浸润深度为T1+T2的胃癌患者(P0.05)。80例胃癌患者cyclinD1阳性表达45例(56.25%),阴性表达35例(43.75%),MMP-9阳性表达65例(81.25%),阴性表达15例(18.75%),TNM临床分期为III+IV期、分化程度为低分化、有淋巴结转移、浸润深度为T3+T4的胃癌患者的cyclinD1、MMP-9阳性表达率明显高于TNM临床分期为I+II期、分化程度为中高分化、无淋巴结转移、浸润深度为T1+T2的胃癌患者(P0.05)。HP感染阳性患者的cyclinD1阳性表达率和MMP-9阳性表达率均明显高于HP感染阴性患者(P0.05)。Pearson相关分析显示,胃癌患者HP感染与cyclinD1、MMP-9表达均呈正相关(P0.05)。结论:胃癌患者的HP感染情况与淋巴结转移、浸润深度有关,cyclinD1和MMP-9的表达与TNM临床分期、分化程度、淋巴结转移、浸润深度有关,且胃癌患者HP感染与cyclinD1、MMP-9表达均呈正相关。     

HepG2细胞中线粒体形状的动态变化

目的:研究HepG2细胞中线粒体形状动态变化过程中的功能变化及其初步分子机制。方法:HepG2细胞经过HBSS缓冲液饥饿处理后,使用线粒体氧化磷酸化解偶联剂CCCP、脂肪酸受体GPR40/120激动剂GW9508、脂肪酸油酸OA和钙离子载体Ionomycin等4种不同药物处理,通过共聚焦显微镜观察和流式细胞分析的手段检测细胞中线粒体形状和功能发生的改变。然后,通过基因沉默Drp1,Mff或者Fis1蛋白,初步研究调控线粒体形状改变的分子机制。结果:经过CCCP和GW9508处理细胞中产生甜甜圈线粒体,而OA和Ionomycin处理产生球状线粒体。CCCP,OA和Ionomycin使线粒体去极化,CCCP、GW9508、OA或者Ionomycin单独处理在一定程度上影响细胞中活性氧化簇ROS。甜甜圈线粒体产生由Drp1介导,而球状线粒体形成依赖于Drp1和Mff。结论:线粒体的形态与其功能相互联系,Drp1和Mff蛋白对于细胞线粒体形状动态改变过程中形状的调整和适应具有很重要的作用。     

白藜芦醇通过下调MEK/ERK/c-Jun信号通路抑制H2O2诱导的肺癌细胞增殖

目的:探讨白藜芦醇(Res)是否通过下调ERK激酶/胞外信号调节激酶/原癌基因(MEK/ERK/c-Jun)信号通路抑制小剂量过氧化氢(H2O2)诱导肺癌细胞增殖。方法:采用MTS实验检测小剂量20μM H2O2以及分别加入MEK阻断剂U0126和Res后H2O2对肺癌细胞NCI-H1395增殖的影响,采用Western Blot检测H2O2对ERK1/2和Akt蛋白磷酸化水平以及加入Res后H2O2对MEK、ERK1/2和c-Jun蛋白磷酸化水平的影响。结果:小剂量H2O2对肺癌细胞NCI-H1395具有促增殖作用,H2O2通过活化ERK1/2和Akt蛋白的磷酸化水平促进肺癌细胞NCI-H1395增殖,加入MEK阻断剂U0126后H2O2对肺癌细胞NCI-H1395增殖作用降低(P<0.05)。Res可抑制H2O2诱导的肺癌细胞NCI-H1395增殖,加入Res后,H2O2引起的MEK、ERK1/2和c-Jun蛋白磷酸化水平均降低(P<0.05)。结论:小剂量H2O2对肺癌细胞NCI-H1395具有促增殖作用,Res通过抑制MEK/ERK/c-Jun信号通路来抑制H2O2对肺癌细胞NCI-H1395的促增殖作用,其具体机制还需进一步研究。     

超声引导下腔内射频消融联合点式剥脱治疗下肢静脉曲张的临床效果观察

目的:探讨超声引导下腔内射频消融(radiofrequency endovenous obliteration,RFO)联合点式剥脱治疗下肢静脉曲张的临床疗效及安全性。方法:选取2017年10月至2018年10月哈尔滨医科大学附属第一医院收治的下肢静脉曲张患者共320例(320条肢体),将其随机分为观察组200例和对照组120例。观察组200例(200条肢体)采用超声引导下腔内射频消融联合点式剥脱进行治疗,对照组120例(120条肢体)采用传统大隐静脉高位结扎剥脱、曲张静脉点式剥脱治疗。比较两组患者平均住院时间、术后并发症的发生情况及术后1年下肢静脉的彩超随访情况。结果:观察组患者平均住院时间较对照组显著缩短,术后皮肤瘀斑、皮下血肿、隐神经损伤、切口感染的发生率显著低于对照组(P0.05),两组患者术后1年复查,其复发率及大隐静脉主干闭塞情况比较差异没有统计学意义(P0.05)。结论:与传统大隐静脉高位结扎剥脱、曲张静脉点式剥脱治疗相比,超声引导下腔内射频消融联合点式剥脱治疗下肢静脉曲张的疗效及安全性均更高,有利于患者术后康复。     

小骨窗显微手术治疗基底节区高血压脑出血的效果分析及对血清CCCK-18、CTRP-3水平的影响

目的:探讨小骨窗显微手术治疗基底节区高血压脑出血的效果及对血清gaspase切割的细胞角蛋白18(CCCK-18)、补体C1q肿瘤坏死因子相关蛋白3(CTRP-3)水平的影响。方法:选取2016年5月至2018年5月我院收治的160例基底节区高血压脑出血患者,按照随机数表法将其分为观察组(n=82)和对照组(n=78)。对照组采用传统大骨瓣开颅术治疗,观察组采用小骨窗显微手术治疗。观察和比较两组的临床疗效,血肿清除率、术中出血量、术后意识恢复时间、住院时间,治疗前后NIHSS、ADL评分、血清CCCK-18、CTRP-3水平的变化及并发症的发生情况。结果:治疗后,观察组总有效率显著高于对照组[95.12%vs. 79.48%](P0.05);血肿清除率、术中出血量、术后意识恢复时间、住院时间均显著优于对照组[(93.62±3.58)%vs.(85.40±2.19)%,(92.47±12.56)mL vs.(189.25±26.47) mL,(2.01±0.58) d vs.(8.69±2.03) d,(13.39±2.08) d vs.(19.45±3.76) d](P0.05);NIHSS评分显著低于对照组[(9.76±1.42)分vs.(20.57±3.26)分](P0.05);ADL评分显著高于对照组[(86.42±8.64)分vs.(75.39±7.02)分](P0.05);血清CCCK-18水平显著低于对照组[(201.76±32.59) U/L vs.(237.57±39.20) U/L,(29.59±5.19) ng/mL vs.(42.97±7.94)ng/mL](P0.05);CTRP-3水平显著高于对照组[(289.59±35.19)ng/mL vs.(232.97±27.94)ng/mL](P0.05);并发症总发生率显著低于对照组[3.65%(3/82) vs. 14.10%(11/78)](P0.05)。结论:小骨窗显微手术治疗基底节区高血压脑出血的疗效显著,可更有效清除血肿,缓解血肿压迫,改善神经功能,减少继发性损伤,安全性高,可能与其降低血清CCCK-18水平及升高CTRP-3水平有关。     

空心钉加内侧支撑钢板与单纯空心钉治疗PauwelsⅢ型股骨颈骨折的疗效比较研究

目的:比较PauwelsⅢ型股骨颈骨折分别经单纯空心钉、空心钉加内侧支撑钢板治疗的临床疗效。方法:回顾性分析2016年3月～2019年2月期间收治的113例PauwelsⅢ型股骨颈骨折患者的临床资料,根据手术方式分为A组(n=55,单纯空心钉内固定治疗)和B组(n=58,空心钉加内侧支撑钢板治疗),比较两组患者围术期指标、髋关节功能、术后疼痛及复位质量,记录两组患者随访期间并发症发生情况。结果:B组术中出血量多于A组,手术时间长于A组(均P0.05);B组骨折愈合时间、完全负重时间短于A组(P0.05)。两组患者术后3个月、术后6个月髋关节Harrris评分呈升高趋势,视觉模拟评分量表(VAS)评分呈下降趋势(P0.05);B组术后3个月、术后6个月髋关节Harrris评分高于A组,VAS评分则低于A组(P0.05)。与术后3 d相比,A组患者术后6个月正位、侧位Garden指数降低(P0.05);B组术后6个月正位、侧位Garden指数评分高于A组(P0.05)。B组并发症发生率低于A组(P0.05)。结论:与单纯空心钉内固定治疗PauwelsⅢ型股骨颈骨折相比,空心钉加内侧支撑钢板虽然术中出血量多,手术时间略长,但其术后恢复效果更佳,且并发症发生率更低,临床应用价值较高。     

滇川桂3省暗褐网柄牛肝菌菌腔虫瘿的寄主植物与介壳虫种类调查

暗褐网柄牛肝菌Phlebopus portentosus与介壳虫形成的菌腔虫瘿是该菌营养机制研究的关键环节。本研究先后在云南、四川和广西3省区暗褐网柄牛肝菌产区的16个地点,对菌腔虫瘿的生态和生物学进行了大量的野外调查。发现根部着生菌腔虫瘿的寄主植物有31种,涉及16个科的28个属。与暗褐网柄牛肝菌形成菌腔虫瘿的介壳虫种类有12种,其中10种隶属粉蚧科Pseudococcidae、绵蚧科Monophlebidae、蚧科Coccidae各1种。在不同的寄主植物上菌腔虫瘿的寄生位置和形状会有所不同,与暗褐网柄牛肝菌菌丝形成菌腔虫瘿的寄主植物和介壳虫之间不存在专一性。上述研究结果为暗褐网柄牛肝菌的仿生栽培奠定了基础。     

Epidemiological,Clinical and Serological Characteristics of Children with Coronavirus Disease 2019 in Wuhan: A Single-centered,Retrospective Study

In December 2019, SARS-CoV-2 was first detected in the samples obtained from three adult patients who suffered from an unknown viral pneumonia in Wuhan (Li et al. 2020). This unknown viral pneumonia is further named as coronavirus disease 2019 (COVID-19) by the World Health Organization. To date, the number of new COVID-19 cases has continued to skyrocket and the impact of SARS-CoV-2 on humans is far greater than any pathogen of this century in both breadth and depth. Previous studies have shown that adults with COVID-19 have symptoms of fever, dry cough, dyspnea, fatigue and lymphocytopenia. Moreover, COVID-19 is more likely to cause death in the elderly, especially those with chronic comorbidities (Huang et al. 2020). In Wuhan, more than 50, 000 COVID-19 cases have been confirmed, including over 780 pediatric patients, and only one child death case (Lu et al. 2020). Although the number of children cases was far fewer than that of adults, COVID-19 might endanger children's health and the information on children remains limited, especially in serological study. In the retrospective study, the investigators analyzed the epidemiological, clinical and serological characteristics of children with COVID-19 in Wuhan in the early stages of the outbreak, which might provide theoretical and practical help in controlling COVID-19 and similar emerging infectious diseases in the future.     

Depth‐dependent soil organic carbon dynamics of croplands across the Chengdu Plain of China from the 1980s to the 2010s

Agricultural soils have tremendous potential to sequester soil organic carbon (SOC) and mitigate global climate change. However, agricultural land use has a profound impact on SOC dynamics, and few studies have explored how agricultural land use combined with soil conditions affect SOC changes throughout the soil profile. Based on a paired soil resampling campaign in the 1980s and 2010s, this study investigated the SOC changes of the soil profile caused by agricultural land use and the correlations with parent material and topography across the Chengdu Plain of China. The results showed that the SOC content increased by 3.78 g C/kg in the topsoil (0–20 cm), but decreased in the 20–40 cm and 40–60 cm soil layers by 0.90 and 1.26 g C/kg respectively. SOC increases in topsoil were observed for all types of agricultural land. Afforestation on former agricultural land also caused SOC decreases in the 20–60 cm soil layers, while SOC decreases only occurred in the 40–60 cm soil layer for agricultural land using a traditional crop rotation (i.e. traditional rice–wheat/rapeseed rotation) and with rice–vegetable rotations converted from the traditional rotations. For each agricultural land use, SOC decreases in deep soils only occurred in high relief areas and in soils formed from Q4 (Quaternary Holocene) grey‐brown alluvium and Q4 grey alluvium that had a relatively low soil bulk density and clay content. The results indicated that SOC change caused by agricultural land use was depth dependent and that the effects of agricultural land use on soil profile SOC dynamics varied with soil characteristics and topography. Subsoil SOC decreases were more likely to occur in high relief areas and in soils with low soil bulk density and low clay content.