全文获取类型
收费全文 | 642689篇 |
免费 | 67800篇 |
国内免费 | 424篇 |
专业分类
710913篇 |
出版年
2016年 | 7040篇 |
2015年 | 9476篇 |
2014年 | 11034篇 |
2013年 | 16652篇 |
2012年 | 18013篇 |
2011年 | 18678篇 |
2010年 | 12595篇 |
2009年 | 11636篇 |
2008年 | 16818篇 |
2007年 | 17556篇 |
2006年 | 16613篇 |
2005年 | 15848篇 |
2004年 | 15797篇 |
2003年 | 15422篇 |
2002年 | 15074篇 |
2001年 | 26270篇 |
2000年 | 26365篇 |
1999年 | 21094篇 |
1998年 | 7667篇 |
1997年 | 8198篇 |
1996年 | 7825篇 |
1995年 | 7220篇 |
1994年 | 7060篇 |
1993年 | 7119篇 |
1992年 | 18309篇 |
1991年 | 18126篇 |
1990年 | 17878篇 |
1989年 | 17658篇 |
1988年 | 16477篇 |
1987年 | 15647篇 |
1986年 | 14480篇 |
1985年 | 15088篇 |
1984年 | 12524篇 |
1983年 | 10719篇 |
1982年 | 8345篇 |
1981年 | 7549篇 |
1980年 | 7096篇 |
1979年 | 12050篇 |
1978年 | 9422篇 |
1977年 | 8946篇 |
1976年 | 8439篇 |
1975年 | 9185篇 |
1974年 | 10108篇 |
1973年 | 9940篇 |
1972年 | 9131篇 |
1971年 | 8356篇 |
1970年 | 7333篇 |
1969年 | 7234篇 |
1968年 | 6658篇 |
1967年 | 5778篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
131.
G Rodriguez-Tarduchy P Malde A Lopez-Rivas M K Collins 《Journal of immunology (Baltimore, Md. : 1950)》1992,148(5):1416-1422
Calcium ionophores inhibit apoptosis in the IL-3-dependent cell line BAF3 and maintain the cells in a viable noncycling state. In this report, an identical effect of ionophore was also demonstrated on the multipotent IL-3-dependent progenitor cell line FDCP-MIX and on the primary IL-3-dependent cell population that could be cultured from murine bone marrow. Inhibition of apoptosis required extracellular calcium and could be blocked by cyclosporin A. Nuclei from IL-3-dependent cells were found to lack a calcium-activatable nuclease that degrades chromatin in the linker region between nucleosomes, unlike the nuclei of lymphoid cells. The mechanism of action of calcium ionophore could be divided into two distinct steps. First, ionophore induced the production of a survival factor that stimulated DNA synthesis and was identified as IL-4. Second, ionophore inhibited the cell cycle of the various IL-3-dependent cells. IL-4 production could be inhibited by cyclosporin A and required extracellular calcium, whereas cell cycle arrest did not. This implied that factor production was the step that was necessary for inhibition of apoptosis and maintenance of cell viability. This was confirmed by the use of an anti-IL-4R antibody, which blocked the inhibition of apoptosis induced by calcium ionophores. 相似文献
132.
Structural membrane lipids are known to contribute to the high ethanol resistance of Saccharomyces cerevisiae (2, 4, 17). By manipulating the yeast cellular sterol level by changing the carbon-to-nitrogen source ratio in the chemostat growth medium, high delta 5,7-sterol levels were found to increase the resistance of yeast populations to ethanol-induced death. The resistance of the erg2 (delta 8----delta 7-sterol isomerase) mutant to ethanol-induced death was generally comparable with that of the delta 5,7-sterol-synthesizing strain. In contrast, the sensitivity of anaerobic growth to inhibition by ethanol was higher in the erg2 mutant in comparison with the delta 5,7-sterol-synthesizing strains but a high level of those sterols increased the vulnerability of anaerobic growth to ethanol inhibition. 相似文献
133.
Murine Ia-associated invariant chain's processing to complex oligosaccharide forms and its dissociation from the I-Ak complex 总被引:5,自引:0,他引:5
G D Holt S J Swiedler J H Freed G W Hart 《Journal of immunology (Baltimore, Md. : 1950)》1985,135(1):399-407
The processing of murine invariant chain (Ii) to a cell surface form bearing complex N-linked oligosaccharides has been demonstrated in the B cell lymphoma, AKTB-1b. In addition, the rate of processing of pulse-labeled Ii has been determined relative to its rate of dissociation from the alpha/beta complex of I-Ak. Ii, alpha-, and beta-chains were immunoprecipitated with anti-I-Ak or anti-Ii monoclonal antibodies. The heretofore uncharacterized complex oligosaccharide form of Ii (Ii-c) was identified in gel-purified immunoprecipitates by peptide mapping with reverse-phase HPLC. Ii-c is resistant to deglycosylation by Endo H, which is specific for high-mannose N-linkages, but can be digested with Endo F, a glycosidase capable of cleaving both complex and high-mannose N-linked oligosaccharides. Immunoprecipitation of surface iodinated cells indicates that Ii-c is expressed on the plasma membrane. Pulse-chase metabolic labeling data show that the processing of Ii to Ii-c occurs with a t1/2 of about 120 min. In contrast, the processing of both alpha- and beta-chains of I-Ak to complex forms occurs with a t1/2 of 15 to 20 min. Our data show that Ii-hm begins to dissociate rapidly from the I-Ak complex after 100 to 120 min of chase. Only a small amount (less than 5% on a per mole basis) of Ii-c was found associated with the I-Ak complexes after 300 min of continuous metabolic labeling. These results are consistent with Ii serving as a carrier for Ia antigens as they are transported to the cell surface. In addition, they suggest that the processing of Ii to Ii-c, or a late processing event of the alpha- and beta-chains, such as their sialylation, may be a possible mechanism for inducing the dissociation of Ii from the I-Ak complex. 相似文献
134.
135.
136.
Forty-two white-rot fungi isolated in South America were incubated with long fibre sugar cane bagasse (LFB). The residual composition of LFB was determined after white-rot decay at 30 and 60 days. The ratio of residual lignin to residual lignin to residual cellulose (RL/RC) of untreated material (LFB) was 0.48. After white-rot-decay, the residual material with lower RL/RC ratios indicated that mainly lignin was degraded. In only 30 days, Phlebia sp. MVHC 5535, Athelia sp. MVHC 5509 and Spongipellis pachyodon MVHC 5019 caused a decrease in the RL/RC ratio to 0.36, 0.37 and 0.38, respectively, while it took 60 days for Ganoderma applanatum MVHC 5347, Hyphodontia sp. MVHC 5544, Panus tigrinus MVHC 5400, Stereum sp. MVHC 5113, Phellinus punctatus MVHC 5346 and MVHC 6388 to reach a ratio lower than 0.40. No correlation was found between the amount of some ligninolytic enzymes secreted and the residual composition of bagasse after white-rot fungi fermentation. Most of the fungal strains caused an increase in the relative amount of residual cellulose, indicating that hemicellulose was the preferred energy source. 相似文献
137.
138.
Gastrointestinal pH measurement in rats: influence of the microbial flora, diet and fasting 总被引:1,自引:0,他引:1
The pH of the rat intestinal tract was decreased by the presence of a microbial flora, but its influence in the forestomach is less clear. Stomach pH values varied according to the amount of food present at the time of measurement. Fasting increased the pH of the gastrointestinal tract in conventional rats but had little effect in germfree rats. In the conventional rat, feeding a purified diet compared with a commercial diet resulted in a lower pH in the forestomach and a higher pH in the caecal contents. Magnesium trisilicate promoted gastric emptying in conventional rats and its antacid effect was observed only in the caecum and colon. 相似文献
139.
140.