全文获取类型
收费全文 | 1604660篇 |
免费 | 152724篇 |
国内免费 | 1555篇 |
出版年
2021年 | 18150篇 |
2019年 | 16293篇 |
2018年 | 18913篇 |
2017年 | 17500篇 |
2016年 | 29004篇 |
2015年 | 43346篇 |
2014年 | 51483篇 |
2013年 | 77897篇 |
2012年 | 45237篇 |
2011年 | 35721篇 |
2010年 | 46542篇 |
2009年 | 47121篇 |
2008年 | 32920篇 |
2007年 | 31774篇 |
2006年 | 35234篇 |
2005年 | 36190篇 |
2004年 | 35366篇 |
2003年 | 32731篇 |
2002年 | 30711篇 |
2001年 | 48049篇 |
2000年 | 45809篇 |
1999年 | 42078篇 |
1998年 | 27257篇 |
1997年 | 27139篇 |
1996年 | 26430篇 |
1995年 | 24499篇 |
1994年 | 24287篇 |
1993年 | 23509篇 |
1992年 | 35932篇 |
1991年 | 34256篇 |
1990年 | 33100篇 |
1989年 | 33605篇 |
1988年 | 31007篇 |
1987年 | 29429篇 |
1986年 | 27705篇 |
1985年 | 29438篇 |
1984年 | 27801篇 |
1983年 | 24529篇 |
1982年 | 23304篇 |
1981年 | 22146篇 |
1980年 | 20661篇 |
1979年 | 24185篇 |
1978年 | 21492篇 |
1977年 | 20502篇 |
1976年 | 19647篇 |
1975年 | 19518篇 |
1974年 | 20458篇 |
1973年 | 20690篇 |
1972年 | 17948篇 |
1971年 | 16487篇 |
排序方式: 共有10000条查询结果,搜索用时 593 毫秒
861.
862.
863.
864.
865.
866.
867.
868.
The present study was undertaken to comparatively investigate the attachment capacities of Azospirillum brasilenseSp245 and its lipopolysaccharide-defective Omegon-Km mutants KM018 and KM252, as well as their activities with respect to the alteration of the morphology of wheat seedling root hairs. The adsorption dynamics of the parent Sp245 and mutant KM252 strains of azospirilla on the seedling roots of the soft spring wheat cv. Saratovskaya 29 were similar; however, the attachment capacity of the mutant KM252 was lower than that of the parent strain throughout the incubation period (15 min to 48 h). The mutation led to a considerable decrease in the hydrophobicity of the Azospirillumcell surface. The lipopolysaccharides extracted from the outer membrane of A. brasilenseSp245 and mutant cells with hot phenol and purified by chromatographic methods were found to induce the deformation of the wheat seedling root hairs, the lipopolysaccharide of the parent strain being the most active in this respect. The role of the carbohydrate moiety of lipopolysaccharides in the interaction of Azospirillumcells with plants is discussed. 相似文献
869.
Haems are the cofactors of cytochromes and important catalysts of biological electron transfer. They are composed of a planar porphyrin structure with iron coordinated at the centre. It is known from spectroscopy that ferric low-spin haem has one unpaired electron at the iron, and that this spin is paired as the haem receives an electron upon reduction (I. Bertini, C. Luchinat, NMR of Paramagnetic Molecules in Biological Systems, Benjamin/Cummins Publ. Co., Menlo Park, CA, 1986, pp. 165-170; H.M. Goff, in: A.B.P. Lever, H.B. Gray (Eds.), Iron Porphyrins, Part I, Addison-Wesley Publ. Co., Reading, MA, 1983, pp. 237-281; G. Palmer, in: A.B.P. Lever, H.B. Gray (Eds.), Iron Porphyrins, Part II, Addison-Wesley Publ. Co., Reading, MA, 1983, pp. 43-88). Here we show by quantum chemical calculations on a haem a model that upon reduction the spin pairing at the iron is accompanied by effective delocalisation of electrons from the iron towards the periphery of the porphyrin ring, including its substituents. The change of charge of the iron atom is only approx. 0.1 electrons, despite the unit difference in formal oxidation state. Extensive charge delocalisation on reduction is important in order for the haem to be accommodated in the low dielectric of a protein, and may have impact on the distance dependence of the rates of electron transfer. The lost individuality of the electron added to the haem on reduction is another example of the importance of quantum mechanical effects in biological systems. 相似文献
870.
Membrane targeting of RecA during genetic transformation 总被引:2,自引:1,他引:1
H. Robert Masure Barbara J. Pearce Helen Shio & Barbara Spellerberg 《Molecular microbiology》1998,27(4):845-852
Recombination in prokaryotes and eukaryotes is mediated by the RecA family of proteins. Although the interactions between RecA and DNA are well studied, the cellular location of these interactions is not known. Using genetic transformation of Streptococcus pneumoniae as a model system, there was increased expression of a protein, colligrin, and RecA, products of the rec locus during genetic transfer. These proteins formed a complex and were found associated with the membranes of genetically competent cells. With immunoelectron microscopy and subcellular fractionation, we showed that the induction of competence led to the translocation of RecA and colligrin to the membrane and to the formation of clusters of RecA in a colligrin-dependent step. Based on the behaviour of colligrin and RecA during genetic exchange and the numerous proteins in prokaryotes and eukaryotes with domains similar to colligrin, we suggest that there may exist a family of proteins, which gathers macromolecules at specific sites in biological membranes. 相似文献