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排序方式: 共有169条查询结果,搜索用时 15 毫秒
91.
Masashi Yoshida Satoshi Katsuyama Kazuki Tateho Hiroto Nakamura Junpei Miyoshi Tatsunori Ohba Hirotada Matsuhara Futaba Miki Koei Okazaki Tokuko Haraguchi Osami Niwa Yasushi Hiraoka Ayumu Yamamoto 《The Journal of cell biology》2013,200(4):385-395
During meiosis, telomeres cluster and promote homologous chromosome pairing. Telomere clustering requires the interaction of telomeres with the nuclear membrane proteins SUN (Sad1/UNC-84) and KASH (Klarsicht/ANC-1/Syne homology). The mechanism by which telomeres gather remains elusive. In this paper, we show that telomere clustering in fission yeast depends on microtubules and the microtubule motors, cytoplasmic dynein, and kinesins. Furthermore, the γ-tubulin complex (γ-TuC) is recruited to SUN- and KASH-localized telomeres to form a novel microtubule-organizing center that we termed the “telocentrosome.” Telocentrosome formation depends on the γ-TuC regulator Mto1 and on the KASH protein Kms1, and depletion of either Mto1 or Kms1 caused severe telomere clustering defects. In addition, the dynein light chain (DLC) contributes to telocentrosome formation, and simultaneous depletion of DLC and dynein also caused severe clustering defects. Thus, the telocentrosome is essential for telomere clustering. We propose that telomere-localized SUN and KASH induce telocentrosome formation and that subsequent microtubule motor-dependent aggregation of telocentrosomes via the telocentrosome-nucleated microtubules causes telomere clustering. 相似文献
92.
Kenji Kitamura Tomohiro Nakamura Futaba Miki Chikashi Shimoda 《FEMS microbiology letters》1996,143(1):41-45
Abstract The mating response of the fission yeast Schizosaccharomyces pombe is mediated by mating pheromones, M-factor and P-factor, produced by h− and h+ cells, respectively. When the M-factor receptor (Map3) was ectopically expressed in h− cells lacking the P-factor receptor (Mam2), they acquired mating competence in response to M-factor which they secreted. The autocrine response to P-factor in h+ cells was so weak that mating competence was not acquired, although expression of the pheromone-responsive gene mat1-Pm was detected. These observations support the notion that the intensity of cellular response to mating pheromones is different between h− and h+ cells, although downstream pathways of the pheromone receptors are shared by the two mating types. 相似文献
93.
AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5, and also promotes atpF splicing in Arabidopsis and rice 下载免费PDF全文
94.
Kazama T Fujie M Endo T Kano K 《Biochemical and biophysical research communications》2008,377(3):780-785
We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into osteoblasts and chondrocytes under appropriate culture conditions. However, it is unclear whether DFAT cells are capable of transdifferentiating into skeletal myocytes, which is common in the mesodermal lineage. Here, we show that DFAT cells can be induced to transdifferentiate into skeletal myocytes in vitro. Myogenic induction of DFAT cells resulted in the expression of MyoD and myogenin, followed by cell fusion and formation of multinucleated cells expressing sarcomeric myosin heavy chain. These results indicate that DFAT cells derived from mature adipocytes can transdifferentiate into skeletal myocytes in vitro. 相似文献
95.
Toshitada Kazama Minoru Takagi Teruhiko Ishii Yoshihisa Toda 《The Histochemical journal》1992,24(10):747-755
Summary The types and distribution of glycosaminoglycans (GAGs) were studied immunocytochemically in osteoid, mineralized bone matrix, and cartilage matrix of growing rat metaphyseal bone after aldehyde fixation and EDTA demineralization, using four monoclonal antibodies (mAbs 1-B-5, 2-B-6, 3-B-3 and 5-D-4). These mAbs specifically recognize epitopes in non-sulphated chondroitin (C0-S); chondroitin 4-sulphate (C4-S) and dermatan sulphate (DS); chondroitin 6-sulphate (C6-S) and C0-S; and keratan sulphate (KS) respectively. In osteoid, all mAbs except 1-B-5 weakly stained matrix material on and between collagen fibrils, and moderately stained organic material corresponding to bone nodules, which are known sites of mineralization. However, the staining of osteoid abruptly decreased at the mineralization front; weak staining was confined mostly to the organic material of bone nodules in mineralized bone matrix, with very weak or no staining of the rest of the bone matrix. This staining progressively decreased toward the mineralized cartilage matrix and became negative. The mineralized cartilage matrix and lamina limitans reacted strongly with all mAbs except 5-D-4. These results indicate that osteoid contains sulphated proteoglycans containing C4-S and/or DS, C6-S and KS, and subsequent bone matrix mineralization appears to require accumulation of these macromolecules within bone nodules and eventual loss of these substances for complete mineralization, whereas proteoglycans containing C0-S, C4-S and/or DS, and C6-S, still exist in mineralized cartilage matrix and lamina limitants. 相似文献
96.
IAA-induced growth of light-grown cucumber hypocotyl sectionsis markedly enhanced by GA3-pretreatment of the sections; thereis a distinct synergism between IAA and GA3. Water pretreatmentalso enhances IAA-induced growth. On the other hand, IAA-pretreatedsections showed practically no further growth in response topost treatment with GA3. The enhancing effect of GA3 is obtainedwith only 30 min pretreatment, the maximum effect occuring with2 hr pretreatment. Pretreatment longer than 8 hr is less effective.This enhancing effect of GA3 can be observed soon after posttreatment with IAA. The response of GA3-pretreated sectionsto IAA is greater in pretreatment with higher concentrationsof GA3, and higher degrees of synergism between IAA and GA3are obtained at IAA concentrations less than 10-4 M. This synergisticinteraction between GA3 and IAA is more marked in aged hypocotylsections than in young sections. From these results we concludedthat gibberellin sensitizes hypocotyl cells to the subsequenteffect of auxin on cell elongation. (Received October 6, 1973; ) 相似文献
97.
98.
Kentaro Kazama Kazuhiko Hirata Takashi Yamamoto Hiroshi Hashimoto Akinori Takahashi Yasuaki Niizuma Philip N. Trathan Yutaka Watanuki 《Journal of avian biology》2013,44(6):603-608
Long‐lived animals sometimes skip one or more breeding seasons; however, little is known about their movements and activities during such ‘sabbatical’ periods. Here we present novel data on year‐round movements and activities of two male black‐tailed gulls Larus crassirostris during a sabbatical year. We compare the data with those in a year when they bred and with those of two other breeding males. The year‐round migration routes of two sabbatical males were consistent with those of the breeding males: they returned to the breeding area but did not visit the colony in the sabbatical year. They landed more frequently on water (a potential index of foraging effort) during the non‐breeding autumn and winter prior to the sabbatical year than before breeding. Sabbatical gulls may forage more intensively to recover body condition immediately after breeding. 相似文献
99.
100.
In vitro PGI2 synthesis by aortic strips obtained from thoracic aorta of rabbits fed a high cholesterol diet was examined and compared with that of control rabbits fed a normal diet. In this report, the amounts of PGI2 produced were shown as 6-keto-PGF1 alpha per microgram of aortic tissue DNA instead of per mg wet weight. We also investigated PGI2 synthesis by cultured smooth muscle cells (SMC) obtained from atherosclerotic intima. Basal PGI2 production by aortic strips from atherosclerotic rabbit aorta was significantly augmented compared with that of controls. Arachidonic acid (AA)-induced PGI2 production by atherosclerotic aorta was also significantly higher than that of controls. PGI2 producing capacities of intimal and medial layers, separated from atherosclerotic aorta, were examined and the intimal layer was found to elicit a significantly greater PGI2 production than the medial layer. Furthermore, cultured intimal SMC obtained from atherosclerotic rabbit aorta produced a greater amount of PGI2 than medial SMC from normal rabbit aorta at various cultured conditions. These results suggest that the possibility of enhanced PGI2 production by atherosclerotic aorta may well be considered as a defence mechanism of the vessel wall against damaging stimuli. 相似文献