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141.
Fusao Ota Junji Morita Nagayuki Yoshida Fraser Ashton Benito Diena 《Microbiology and immunology》1975,19(2):149-155
Macrophages from mice were infected with Neisseria gonorrhoeae type 1 cells, and their ultrastructure was studied by electron microscopy. The macrophages showed various stages of engulfment and digestion of gonococci 2 hr after infection. Infected macrophages seemed to develop pseudopodia for phagocytosis, and could engulf more than 30 gonococcal cells. Some engulfed bacteria appeared morphologically intact, while others appeared lysed and some structures resembling the L form of N. gonorrhoeae were also seen. These observations suggest that gonococcal cells may be able to survive intracellularly with normal or altered forms of morphology, and that macrophages containing these bacteria may disseminate gonococcal infection in man. 相似文献
142.
143.
Egg size of skippers (Lepidoptera: Hesperiidae) in relation to their host specificity and to leaf toughness of host plants 总被引:6,自引:0,他引:6
Fusao Nakasuji 《Ecological Research》1987,2(2):175-183
The adaptive significance of egg size of skippers (Lepidoptera; Hesperiidae) in Japan was evaluated in relation to the leaf
toughness of their major host grasses. The hesperids that fed on tougher grasses laid larger eggs. Hesperids that laid larger
eggs were larger in body size, but lower in fecundity. They also had a wider host range. Thus, despite the lower fecundity,
hesperids may benefit from large eggs by having a wider host range of larvae. Grass feeders had wider range of host plants
than broadleaf feeders. 相似文献
144.
145.
146.
Masayuki Yamato Yuko Takahashi Hiroyuki Tomotake Fusao Ota Katsuhiko Hirota Kazuo Yamaguchi 《Microbiology and immunology》1994,38(3):177-182
Two hybridomas producing monoclonal antibodies (MAbs) were prepared by fusing myeloma cells (Sp2/0-Ag14) with mouse spleen cells immunized with purified spirosin from Yersinia enterocolitica SYT-11–72 (YE72). The antibodies produced by them were designated MAbs-S5 and S27. They were IgG2a and IgG1, respectively, both with χ light chains. MAbs-S5 and S27 reacted specifically with spirosin from YE72. On Western blotting after limited proteolysis with Staphylococcus aureus V8 protease, YE72 spirosin revealed peptide fragments of 35 and 37 kDa reacting markedly with MAb-S5, which suggested the presence of an antigenic determinant on these fragments. By cellular fractionation of YE72 and subsequent EIA and Western blot analysis, spirosome was shown to be present in the cytoplasm of YE72. 相似文献
147.
148.
The tongue of the striped dolphin, Stenella coeruleoalba, shows a V-shaped row of pits on its posterior dorsum. Their development is described on the basis of macroscopic and light microscopic observations on fetal, young, and adult stages. Four to eight pits occur, most often five in the adult. Anlagen of the pits first protrude as round epithelial thickenings which later increase in diameter and become thin. The circular primordia then sink, and grooves oriented both circularly and radially develop in the walls of the shallow pits thus formed. Pits and grooves deepen with development so that older pits become lined with conical projections. As pits grow further, they become elongated anterolaterally, retaining slit-like openings. Each pit in the adult is 2–8 mm long and about 1 mm wide. The pits are not derived from lingual gland ducts but develop independently. Taste buds resembling those of other mammalian tongues can be found in young dolphins but are few in number and limited to the thin epithelium of the pit projections and to that of the side wall of the pits. They first appear in the late prenatal period but degenerate in the adult. A rich nerve supply is observable in the lamina propria below taste buds in the calf. The pits and their projections in the dolphin correspond to the vallate papillae of other mammals, but whether each projection or a whole pit corresponds to a single vallate papilla is undecided. 相似文献
149.
Fusao Motoyoshi 《In vitro cellular & developmental biology. Plant》1993,29(1):13-16
Summary An F1 hybrid betweenLycopersicon esculentum andL. peruvianum was transformed using a Ti-plasmid binary vector with a coat protein gene cDNA of an attenuated tomato mosaic virus (ToMV)
strain L11A which was expressible by the 35S promoter of cauliflower mosaic virus (CaMV). A transgenic plant which expressed the most
resistance to ToMV was chosen as a material to be tested in a nonisolated greenhouse and in the field. This transgenic tomato
plant was propagated by cutting. In the first test using an isolated greenhouse and in the second test conducted in an nonisolated
greenhouse, no major morphologic and physiologic differences were found between the transgenic plants and the nontransgenic
control plants. Also, there was no evidence that the transgenic plants produced any new hazardous substances. Both the transgenic
and the nontransgenic plants were self-sterile, and crossing of the cultivated species with pollen of these plants produced
few seeds. These features of the transgenic plants satisfied the requirements for a small scale field test. The field test
of the transgenic plants are in progress.
Presented in the Session-in-Depth “Field Test Requirements and Performance of Transgenic Plants” at the 1991 World Congress
on Cell and Tissue Culture, Anaheim, California, June 16–20, 1991. 相似文献