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181.
Rie Nakamoto-Matsubara Mamiko Sakata-Yanagimoto Terukazu Enami Kenichi Yoshida Shintaro Yanagimoto Yusuke Shiozawa Tohru Nanmoku Kaishi Satomi Hideharu Muto Naoshi Obara Takayasu Kato Naoki Kurita Yasuhisa Yokoyama Koji Izutsu Yasunori Ota Masashi Sanada Seiichi Shimizu Takuya Komeno Yuji Sato Takayoshi Ito Issay Kitabayashi Kengo Takeuchi Naoya Nakamura Seishi Ogawa Shigeru Chiba 《PloS one》2014,9(10)
Angioimmunoblastic T-cell lymphoma (AITL) and peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) are subtypes of T-cell lymphoma. Due to low tumor cell content and substantial reactive cell infiltration, these lymphomas are sometimes mistaken for other types of lymphomas or even non-neoplastic diseases. In addition, a significant proportion of PTCL-NOS cases reportedly exhibit features of AITL (AITL-like PTCL-NOS). Thus disagreement is common in distinguishing between AITL and PTCL-NOS. Using whole-exome and subsequent targeted sequencing, we recently identified G17V RHOA mutations in 60–70% of AITL and AITL-like PTCL-NOS cases but not in other hematologic cancers, including other T-cell malignancies. Here, we establish a sensitive detection method for the G17V RHOA mutation using a quantitative allele-specific polymerase chain reaction (qAS-PCR) assay. Mutated allele frequencies deduced from this approach were highly correlated with those determined by deep sequencing. This method could serve as a novel diagnostic tool for 60–70% of AITL and AITL-like PTCL-NOS. 相似文献
182.
Kunihiko Watanabe Yukiko Miyoshi Fumiyasu Kubo Ryo Onuma Shauna Murray Takeo Horiguchi 《Phycological Research》2014,62(2):125-135
A new heterotrophic sand‐dwelling dinoflagellate, Ankistrodinium armigerum K. Watanabe, Miyoshi, Kubo, Murray et Horiguchi sp. nov., is described from Ishikari Beach, Hokkaido, Japan and Port Botany, NSW, Australia. The dinoflagellate is laterally compressed, possessing a short triangular epicone and a large sac‐like hypocone. It possesses a right‐handed cingulum and a deeply‐incised sulcus. The sulcus descends towards the posterior of the cell where it becomes much deeper and wider, resulting in a bilobed ventral side to the hypocone, with a greater excavation of the left lobe than the right. In addition, the right lobe of the hypocone is shorter than the left lobe, which allows a partial view of the left sulcal wall when the cell is viewed from its right side. The sulcus ascends in the epicone to form an apical groove. The apical groove is linear but terminates in an ellipsoid fashion and its extremity approaches, but does not form a closed loop with the apical end of the linear portion. The dinoflagellate possesses two distinct size classes of trichocysts. The large trichocysts are located in the posterior part of the cell, while small trichocysts are distributed throughout the cell. The dinoflagellate shares morphological characteristics with the heterotrophic sand‐dwelling dinoflagellate, Ankistrodinium semilunatum, the type species of the genus. These include a laterally compressed cell, a right‐handed cingulum, a deeply‐incised sulcus and the same basic structure to the apical groove. Molecular phylogenetic analyses based on small and large subunits of rDNA showed that in both trees, A. semilunatum and A. armigerum formed a robust clade, suggesting that these two species are closely related. Because no organism with the characteristics of this species exists and because this species is closely related to A. semilunatum, we concluded that this species should be described as a second species of the genus Ankistrodinium. 相似文献
183.
Tomohiro Nishimura Risa Yagi Mariko Usuda Kenji Oda Mai Yamazaki Sayaka Suda Yu Takahashi Fumiyasu Okazaki Yoshimichi Sai Kei Higuchi Tetsuo Maruyama Masatoshi Tomi Emi Nakashima 《生物化学与生物物理学报:生物膜》2014
Betaine uptake is induced by hypertonic stress in a placental trophoblast cell line, and involvement of amino acid transport system A was proposed. Here, we aimed to identify the subtype(s) of system A that mediates hypertonicity-induced betaine uptake. Measurement of [14C]betaine uptake by HEK293 cells transiently transfected with human or rat sodium-coupled neutral amino acid transporters (SNATs), SNAT1, SNAT2 and SNAT4 revealed that only human and rat SNAT2 have betaine uptake activity. The Michaelis constants (Km) of betaine uptake by human and rat SNAT2 were estimated to be 5.3 mM and 4.6 mM, respectively. Betaine exclusively inhibited the uptake activity of SNAT2 among the rat system A subtypes. We found that rat SNAT1, SNAT2 and SNAT4 were expressed at the mRNA level under isotonic conditions, while expression of SNAT2 and SNAT4 was induced by hypertonicity in TR-TBT 18d-1 cells. Western blot analyses revealed that SNAT2 expression on plasma membrane of TR-TBT 18d-1 cells was more potently induced by hypertonicity than that in total cell lysate. Immunocytochemistry confirmed the induction of SNAT2 expression in TR-TBT 18d-1 cells exposed to hypertonic conditions and indicated that SNAT2 was localized on the plasma membrane in these cells. Our results indicate that SNAT2 transports betaine, and that tonicity-sensitive SNAT2 expression may be involved in regulation of betaine concentration in placental trophoblasts. 相似文献
184.
Kasama T Muramatsu M Kobayashi K Yajima N Shiozawa F Hanaoka R Miwa Y Negishi M Ide H Adachi M 《Cellular immunology》2002,219(2):131-139
To further understand the regulatory mechanisms involved in the process of angiogenesis, the present study was designed to determine the expression and regulation of interferon gamma-inducible protein 10 (IP-10) in peripheral blood monocytes and human umbilical vein endothelial cells (HUVECs). We found that the interaction of monocytes with HUVECs resulted in synergistic increases in IP-10 expression and secretion, which consequently inhibited endothelial tube formation in vitro. Induction of IP-10 was mediated via specific cell surface molecules, as indicated by the finding that IP-10 secretion was significantly inhibited by anti-CD40 ligand antibody, and to a lesser extent by anti-CD40 antibody. Furthermore, we examined the effects of soluble mediators, such as inflammatory and immune cytokines on IP-10 secretion. Addition of interleukin (IL)-1, as well as interferon gamma, induced a marked augmentation of IP-10 secretion by unstimulated monocytes, unstimulated HUVECs, and co-cultures of the two cell types. In contrast, IL-10, recognized as an anti-inflammatory cytokine, significantly inhibited IP-10 secretion by co-cultures. Our results suggest that the interaction of monocytes with endothelial cells results in synergistic increases in IP-10 expression and secretion, which contribute to the regulation of angiogenesis and initiation of inflammatory vascular diseases. 相似文献
185.
A series of truncated gene products from an alkaline cellulase from an alkalophilic Bacillus sp. No. 1139 has been prepared. The variously sized proteins were products of in vitro insertional mutagenesis constructs made by gene inserts containing translational terminators. One product, a 46-kDa protein, which had about half the Mr of the original cellulase, had a similar enzyme activity and pH optimum to the original 92-kDa protein. In contrast, a slightly smaller product protein (43 kDa) did not show cellulase activity. 相似文献
186.
Watanabe Masao; Shiozawa Hideyuki; Isogai Akira; Suzuki Akinori; Takeuchi Takuji; Hinata Kokichi 《Plant & cell physiology》1991,32(7):1039-1047
Experimental evidence is presented that there exists an antherprotein that is reactive with a polyclonal antiserum raisedagainst the stigma S-glycoprotein of the S8-homozygote of Brassicacampestris. The antiserum did not react with extracts of seeds,ovaries or leaves. Since this antiserum did not react with thepolysaccharide residues similar to those in S-glycoprotein,it was considered capable of identifying S-glycoprotein-likeproteins in anthers (SA-protein: S-glycopro-tein-like antherprotein). The SA-protein generated a single distinct band ata pI of about 5.0 on blots of gels after isoelectric focusingand three spots at 29 kDa and 83 kDa on blots of two-dimensionalgels, which were different from those of stigma S-glycoprotein.The SA-protein did not contain polysaccharide residue that reactedwith Con A. No allelic differences in pI were found for theSA-protein within a given species, while such differences arecommon in stigma S-glycoprotein. The SA-protein appeared inanthers at the uninucleate microspore stage which is much earlierthan the stage at which the stigma S-xglycoprotein appears.It is present in anther walls rather than in the pollen of matureanthers. The SA-protein is considered to play an important rolein sporophytic control of self-incompatibility. (Received April 2, 1991; Accepted July 24, 1991) 相似文献
187.
188.
Rieko Oyama Mami Takahashi Fusako Kito Marimu Sakumoto Kumiko Shiozawa Zhiwei Qiao Akihiko Yoshida Makoto Endo Akira Kawai Tadashi Kondo 《In vitro cellular & developmental biology. Animal》2018,54(6):458-467
Primary leiomyosarcoma (LMS) of bone is a rare and aggressive mesenchymal malignancy that differentiates toward smooth muscle. Complete resection is the only curable treatment, and novel therapeutic approaches for primary LMS of bone have long been desired. Patient-derived xenografts (PDXs) and cell lines are invaluable tools for preclinical studies. Here, we established PDXs from a patient with primary LMS of bone and a cell line from an established PDX. Bone primary LMS tissue was subcutaneously implanted into highly immune-deficient mice. After two passages, a piece of the tumor was subjected to tissue culturing, and a morphological evaluation and proteomic analysis were performed on the PDX and the established cell line. Moreover, the responses of the established cell line to anti-cancer drugs were examined. Microscopic observations revealed that the PDX tumors retained their original histology. The cell line was established from the third-generation PDX and named NCC-LMS1-X3-C1. The cells were maintained for over 18 mo and 40 passages. The cells exhibited a spindle shape and aggressive growth. Mass spectrometric protein identification revealed that the original tumor tissue, PDX tumor tissue, and NCC-LMS1-X3-C1 cells had similar but distinct protein expression profiles. We previously established the cell line, NCC-LMS1-C1, from the tumor tissue of same patient. We found that the response to drug treatments was different between NCC-LMS1-X3-C1 and NCC-LMS1-C1, suggesting the heterogeneous traits of tumor cells in the identical tumor tissue. This set of PDXs and stable cell line will be a useful resource for bone LMS research. 相似文献
189.
190.
M Shiozawa Y Hiraoka K Yasuda T Imamura D Sakamuro N Taniguchi M Yamazoe H Yoshikawa 《Gene》1990,87(2):299-303
We have determined expression of human GGT gene encoding gamma-glutamyl transpeptidase (GGT) during fetal development of liver using the Northern-blot analysis with a cloned human GGT cDNA and immunohistochemistry with a monoclonal antibody. GGT mRNA could be detected as early as the 12th week of gestation. It then increased gradually to a peak of approx. threefold the amount at week 12, at week 40, just before birth. The size of the mRNA in the fetal liver was 2.7 kb and mRNA of the same size was detected both in the human fetal kidney and human hepatocellular carcinoma as well as normal adult liver. Immunohistochemical analyses show that GGT increased as the fetal liver developed in parallel with the increase in mRNA. Histochemically, GGT was shown to be located in the wall of bile canaliculi when synthesis was low in early development, but to be distributed, in addition, all over the cell membrane of the fetal hepatocytes when synthesis was high at the later stage of development. 相似文献