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排序方式: 共有289条查询结果,搜索用时 15 毫秒
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Okada Y Shimane K Kochi Y Tahira T Suzuki A Higasa K Takahashi A Horita T Atsumi T Ishii T Okamoto A Fujio K Hirakata M Amano H Kondo Y Ito S Takada K Mimori A Saito K Kamachi M Kawaguchi Y Ikari K Mohammed OW Matsuda K Terao C Ohmura K Myouzen K Hosono N Tsunoda T Nishimoto N Mimori T Matsuda F Tanaka Y Sumida T Yamanaka H Takasaki Y Koike T Horiuchi T Hayashi K Kubo M Kamatani N Yamada R Nakamura Y Yamamoto K 《PLoS genetics》2012,8(1):e1002455
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Shoko Sato Yoshimasa Takizawa Fumika Hoshikawa Mariko Dacher Hiroki Tanaka Hiroaki Tachiwana Tomoya Kujirai Yukari Iikura Cheng-Han Ho Naruhiko Adachi Indu Patwal Andrew Flaus Hitoshi Kurumizaka 《Nucleic acids research》2021,49(15):8934
Giardia lamblia is a pathogenic unicellular eukaryotic parasite that causes giardiasis. Its genome encodes the canonical histones H2A, H2B, H3, and H4, which share low amino acid sequence identity with their human orthologues. We determined the structure of the G. lamblia nucleosome core particle (NCP) at 3.6 Å resolution by cryo-electron microscopy. G. lamblia histones form a characteristic NCP, in which the visible 125 base-pair region of the DNA is wrapped in a left-handed supercoil. The acidic patch on the G. lamblia octamer is deeper, due to an insertion extending the H2B α1 helix and L1 loop, and thus cannot bind the LANA acidic patch binding peptide. The DNA and histone regions near the DNA entry-exit sites could not be assigned, suggesting that these regions are asymmetrically flexible in the G. lamblia NCP. Characterization by thermal unfolding in solution revealed that both the H2A–H2B and DNA association with the G. lamblia H3–H4 were weaker than those for human H3–H4. These results demonstrate the uniformity of the histone octamer as the organizing platform for eukaryotic chromatin, but also illustrate the unrecognized capability for large scale sequence variations that enable the adaptability of histone octamer surfaces and confer internal stability. 相似文献
96.
Memory in Caenorhabditis elegans is mediated by NMDA-type ionotropic glutamate receptors 总被引:1,自引:0,他引:1
Kano T Brockie PJ Sassa T Fujimoto H Kawahara Y Iino Y Mellem JE Madsen DM Hosono R Maricq AV 《Current biology : CB》2008,18(13):1010-1015
Learning and memory are essential processes of both vertebrate and invertebrate nervous systems that allow animals to survive and reproduce. The neurotransmitter glutamate signals via ionotropic glutamate receptors (iGluRs) that have been linked to learning and memory formation; however, the signaling pathways that contribute to these behaviors are still not well understood. We therefore undertook a genetic and electrophysiological analysis of learning and memory in the nematode Caenorhabditis elegans. Here, we show that two genes, nmr-1 and nmr-2, are predicted to encode the subunits of an NMDA-type (NMDAR) iGluR that is necessary for memory retention in C. elegans. We cloned nmr-2, generated a deletion mutation in the gene, and showed that like nmr-1, nmr-2 is required for in vivo NMDA-gated currents. Using an associative-learning paradigm that pairs starvation with the attractant NaCl, we also showed that the memory of a learned avoidance response is dependent on NMR-1 and NMR-2 and that expression of NMDARs in a single pair of interneurons is sufficient for normal memory. Our results provide new insights into the molecular and cellular mechanisms underlying the memory of a learned event. 相似文献
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Eizo Takashima Shinzaburo Takamiya Satoru Takeo Fumika Mi-ichi Hisako Amino Kiyoshi Kita 《Parasitology international》2001,50(4)
Using N2 cavitation, we established a protocol to prepare the active mitochondria from Plasmodium falciparum showing a higher succinate dehydrogenase activity than previously reported and a dihydroorotate-dependent respiration. The fact that fumarate partially inhibited the dihydroorotate dependent respiration suggests that complex II (succinate–ubiquinone reductase/quinol–fumarate reductase) in the erythrocytic stage cells of P. falciparum functions as a quinol–fumarate reductase. 相似文献
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Kei Nagashima Tamae Yoda Tomoko Yagishita Aki Taniguchi Takayoshi Hosono Kazuyuki Kanosue 《Journal of applied physiology》2002,92(3):1029-1035
We examined body core and skin temperatures and thermal comfort in young Japanese women suffering from unusual coldness (C, n = 6). They were selected by interview asking whether they often felt severe coldness even in an air-conditioned environment (20-26 degrees C) and compared with women not suffering from coldness (N, n = 6). Experiments were conducted twice for each subject: 120-min exposure at 23.5 degrees C or 29.5 degrees C after a 40-min baseline at 29.5 degrees C. Mean skin temperature decreased (P < 0.05) from 33.6 +/- 0.1 degrees C (mean +/- SE) to 31.1 +/- 0.1 degrees C and from 33.5 +/- 0.1 degrees C to 31.1 +/- 0.1 degrees C in C and N during the 23.5 degrees C exposure. Fingertip temperature in C decreased more than in N (P < 0.05; from 35.2 +/- 0.1 degrees C to 23.6 +/- 0.2 degrees C and from 35.5 +/- 0.1 degrees C to 25.6 +/- 0.6 degrees C). Those temperatures during the 29.5 degrees C exposure remained at the baseline levels. Rectal temperature during the 23.5 degrees C exposure was maintained at the baseline level in both groups (from 36.9 +/- 0.2 degrees C to 36.8 +/- 0.1 degrees C and 37.1 +/- 0.1 degrees C to 37.0 +/- 0.1 degrees C in C and N). The rating scores of cold discomfort for both the body and extremities were greater (P < 0.05) in C than in N. Thus the augmented thermal sensitivity of the body to cold and activated vasoconstriction of the extremities during cold exposure could be the mechanism for the severe coldness felt in C. 相似文献