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61.
J Daugherty TM Evans T Skillom LE Watson NP Money 《Fungal genetics and biology : FG & B》1998,24(3):354-363
Classical studies on spore release within the Saprolegniaceae (Oomycetes) led to the proposition that different mechanisms of sporangial emptying represent steps in an evolutionary transition series. We have reevaluated this idea in a phylogenetic framework using internal transcribed spacer sequences of four genera. These data were compared with the response to osmotic stress exhibited by each taxon. Saprolegnia emerges as the most basal genus, sister to Achlya, Thraustotheca, and Dictyuchus. Achlya and Thraustotheca are most closely related, while Dictyuchus appears to have evolved along a separate evolutionary lineage. The resulting phylogenetic framework is consistent with the idea that the mechanism of sporangial emptying exhibited by Saprolegnia represents the plesiomorphic condition from which the other mechanisms were derived independently. These alternative mechanisms of spore release may have resulted from a small number of mutations that inhibited axonemal development and altered the temporal and spatial expression of lytic enzymes that degrade the sporangial wall. Copyright 1998 Academic Press. 相似文献
62.
H. Scott Swartzwelder Shawn K. Acheson Kelsey M. Miller Hannah G. Sexton Wen Liu Fulton T. Crews Mary-Louise Risher 《PloS one》2015,10(11)
The long-term effects of intermittent ethanol exposure during adolescence (AIE) are of intensive interest and investigation. The effects of AIE on learning and memory and the neural functions that drive them are of particular interest as clinical findings suggest enduring deficits in those cognitive domains in humans after ethanol abuse during adolescence. Although studies of such deficits after AIE hold much promise for identifying mechanisms and therapeutic interventions, the findings are sparse and inconclusive. The present results identify a specific deficit in memory function after AIE and establish a possible neural mechanism of that deficit that may be of translational significance. Male rats (starting at PND-30) received exposure to AIE (5g/kg, i.g.) or vehicle and were allowed to mature into adulthood. At PND-71, one group of animals was assessed using the spatial-temporal object recognition (stOR) test to evaluate memory function. A separate group of animals was used to assess the density of cholinergic neurons in forebrain areas Ch1-4 using immunohistochemistry. AIE exposed animals manifested deficits in the temporal component of the stOR task relative to controls, and a significant decrease in the number of ChAT labeled neurons in forebrain areas Ch1-4. These findings add to the growing literature indicating long-lasting neural and behavioral effects of AIE that persist into adulthood and indicate that memory-related deficits after AIE depend upon the tasks employed, and possibly their degree of complexity. Finally, the parallel finding of diminished cholinergic neuron density suggests a possible mechanism underlying the effects of AIE on memory and hippocampal function as well as possible therapeutic or preventive strategies for AIE. 相似文献
63.
Summary Errors in chromosome segregation leading to numerical anomalies appear to be unusually frequent in Man and consequently a large proportion of conceptions in our species are aneuploid. Concer has been expressed that this frequency may be increased still further following exposure to inducing substances (trisomigens) present in the environment. We have been developing a fungal test system to screen for such trisomigens and in this paper we report its use in detecting induction following exposure to dimethylsulfoxide (DMSO). In our system DMSO induces segregational errors at both the first and second meiotic division. The results also show that increases in aneuploidy are proportional to the underlying spontaneous frequency. If this finding is generally true it will be especially important to avoid exposure to trisomigens as Man might be especially vulnerable to them. 相似文献
64.
Although critically important, the link between animal life histories and ecosystem energetics is seldom explored. In the
pursuit of ecological simplification, ecosystem properties are typically described by models based on static counts, where
organisms are aggregated into trophic- or size-based groups. Consequently, output is often based on an assumption that larger
group biomass equals greater energetic contribution. Here, we modelled the individual growth of over 58,000 fishes from 74
genera within a coral reef ecosystem to investigate the role and importance of taxon-specific life histories to the division,
spatial distribution and relative contribution of biomass production within 14 coral reef fish families. Rank changes among
families in standing biomass to biomass production indicated that small cryptic families (e.g. Gobiidae and Blenniidae) exhibit
collective growth potentials equal to or exceeding those of many other common families composed of individuals with body-sizes
1–3 orders of magnitude larger. Remaining at high risk of predation throughout their lives as a consequence of their small
size, these cryptic fishes also provide a constant food resource and supply of reproductive energy to coral reefs throughout
the year. Enhanced further by the strength and diversity of their trophic relationships within food webs, the highly productive
nature of these small cryptic fishes suggests they make a substantial contribution to the flow of energy in coral reef ecosystems
via predatory pathways. It appears that life histories leave a strong imprint on ecosystem energy fluxes and illustrate the
importance of incorporating taxon-specific features when assigning values to key ecosystem processes.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
65.
Bax Narissa Novaglio Camilla Maxwell Kimberley H. Meyers Koen McCann Joy Jennings Sarah Frusher Stewart Fulton Elizabeth A. Nursey-Bray Melissa Fischer Mibu Anderson Kelli Layton Cayne Emad Gholam Reza Alexander Karen A. Rousseau Yannick Lunn Zau Carter Chris G. 《Reviews in Fish Biology and Fisheries》2022,32(1):189-207
Reviews in Fish Biology and Fisheries - Humans have relied on coastal resources for centuries. However, current growth in population and increased accessibility of coastal resources through... 相似文献
66.
Anna Wolc Honghua H. Zhao Jesus Arango Petek Settar Janet E. Fulton Neil P. O’Sullivan Rudolf Preisinger Chris Stricker David Habier Rohan L. Fernando Dorian J. Garrick Susan J. Lamont Jack C. M. Dekkers 《遗传、选种与进化》2015,47(1)
Background
Genomic selection (GS) using estimated breeding values (GS-EBV) based on dense marker data is a promising approach for genetic improvement. A simulation study was undertaken to illustrate the opportunities offered by GS for designing breeding programs. It consisted of a selection program for a sex-limited trait in layer chickens, which was developed by deterministic predictions under different scenarios. Later, one of the possible schemes was implemented in a real population of layer chicken.Methods
In the simulation, the aim was to double the response to selection per year by reducing the generation interval by 50 %, while maintaining the same rate of inbreeding per year. We found that GS with retraining could achieve the set objectives while requiring 75 % fewer reared birds and 82 % fewer phenotyped birds per year. A multi-trait GS scenario was subsequently implemented in a real population of brown egg laying hens. The population was split into two sub-lines, one was submitted to conventional phenotypic selection, and one was selected based on genomic prediction. At the end of the 3-year experiment, the two sub-lines were compared for multiple performance traits that are relevant for commercial egg production.Results
Birds that were selected based on genomic prediction outperformed those that were submitted to conventional selection for most of the 16 traits that were included in the index used for selection. However, although the two programs were designed to achieve the same rate of inbreeding per year, the realized inbreeding per year assessed from pedigree was higher in the genomic selected line than in the conventionally selected line.Conclusions
The results demonstrate that GS is a promising alternative to conventional breeding for genetic improvement of layer chickens. 相似文献67.
Species traits and climate velocity explain geographic range shifts in an ocean‐warming hotspot
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Jennifer M. Sunday Gretta T. Pecl Stewart Frusher Alistair J. Hobday Nicole Hill Neil J. Holbrook Graham J. Edgar Rick Stuart‐Smith Neville Barrett Thomas Wernberg Reg A. Watson Dan A. Smale Elizabeth A. Fulton Dirk Slawinski Ming Feng Ben T. Radford Peter A. Thompson Amanda E. Bates 《Ecology letters》2015,18(9):944-953
Species' ranges are shifting globally in response to climate warming, with substantial variability among taxa, even within regions. Relationships between range dynamics and intrinsic species traits may be particularly apparent in the ocean, where temperature more directly shapes species' distributions. Here, we test for a role of species traits and climate velocity in driving range extensions in the ocean‐warming hotspot of southeast Australia. Climate velocity explained some variation in range shifts, however, including species traits more than doubled the variation explained. Swimming ability, omnivory and latitudinal range size all had positive relationships with range extension rate, supporting hypotheses that increased dispersal capacity and ecological generalism promote extensions. We find independent support for the hypothesis that species with narrow latitudinal ranges are limited by factors other than climate. Our findings suggest that small‐ranging species are in double jeopardy, with limited ability to escape warming and greater intrinsic vulnerability to stochastic disturbances. 相似文献
68.
Irene TM Arkesteijn Lucas A Smolders Sandra Spillekom Frank M Riemers Esther Potier Bj?rn P Meij Keita Ito Marianna A Tryfonidou 《Arthritis research & therapy》2015,17(1)
IntroductionEarly degenerative changes in the nucleus pulposus (NP) are observed after the disappearance of notochordal cells (NCs). Thus, it has been suggested that NCs play an important role in maintaining the NP and may have a regenerative potential on other cells of the NP. As the number of resident NP cells (NPCs) decreases in a degenerating disc, mesenchymal stromal (stem) cells (MSCs) may be used for cell supplementation. In this study, using cells of one species, the regenerative potential of canine NCs was assessed in long-term three-dimensional coculture with canine NPCs or MSCs.MethodsCanine NCs and canine NPCs or MSCs were cocultured in alginate beads for 28 days under hypoxic and high-osmolarity conditions. Cell viability, cell morphology and DNA content, extracellular matrix production and expression of genes related to NC markers (Brachyury, KRT18) and NP matrix production (ACAN, COL2A1, COL1A1) were assessed after 1, 15 and 28 days of culture.ResultsNCs did not completely maintain their phenotype (morphology, matrix production, gene expression) during 28 days of culture. In cocultures of NPCs and NCs, both extracellular matrix content and anabolic gene expression remained unchanged compared with monoculture groups, whereas cocultures of MSCs and NCs showed increased glycosaminoglycan/DNA. However, the deposition of these proteoglycans was observed near the NCs and not the MSCs. Brachyury expression in the MSC and NC coculture group increased in time. The latter two findings indicate a trophic effect of MSCs on NCs rather than vice versa.ConclusionsNo regenerative potential of canine NCs on canine NPCs or MSCs was observed in this study. However, significant changes in NC phenotype in long-term culture may have resulted in a suboptimal regenerative potential of these NCs. In this respect, NC-conditioned medium may be better than coculture for future studies of the regenerative potential of NCs.
Electronic supplementary material
The online version of this article (doi:10.1186/s13075-015-0569-6) contains supplementary material, which is available to authorized users. 相似文献69.
70.
Millan DS Ballard SA Chunn S Dybowski JA Fulton CK Glossop PA Guillabert E Hewson CA Jones RM Lamb DJ Napier CM Payne-Cook TA Renery ER Selby MD Tutt MF Yeadon M 《Bioorganic & medicinal chemistry letters》2011,21(19):5826-5830
In this Letter we present data for a novel series of ICS for the treatment of asthma. 'Inhalation by design' principles have been applied to a series of highly potent steroidal GR agonists, with a focus on optimising the potential therapeutic index in human. Pharmacokinetic properties were tuned with high intrinsic clearance and low oral bioavailability in mind, to minimise systemic exposure and reduce systemically driven adverse events. High CYP mediated clearance as well as glucuronidation were targeted to achieve high intrinsic clearance coupled with multiple routes of clearance to minimise drug-drug interactions. Furthermore, pharmaceutical properties such as stability, crystallinity and solubility were considered to ensure compatibility with a dry powder inhaler. This work culminated in the identification of the clinical candidate 15, which demonstrates preclinically the desired efficacy and safety profiles confirming its potential as an inhaled agent for the treatment of asthma. 相似文献