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171.
Fuller BT Molleson TI Harris DA Gilmour LT Hedges RE 《American journal of physical anthropology》2006,129(1):45-54
Historical documents indicate that breastfeeding and weaning practices have fluctuated in England through history. In order to obtain evidence for general breastfeeding patterns in Late/Sub-Roman Britain, stable carbon and nitrogen isotope values were measured in juvenile and adult skeletons (n = 87) from the cemetery of Queenford Farm, Dorchester-on-Thames, Oxfordshire. As the site contained few individuals between 0-1.5 years of age, it was not possible to determine the initial timing for the introduction of weaning foods. Between ages 2-4 years, the mean +/- SD delta(13)C results (-20.2 +/- 0.3 per thousand) are significantly more negative (t = -4.03, P < 0.001) compared to adult females (-19.7 +/- 0.3 per thousand). This is interpreted as evidence of a different diet being fed to children during weaning. After age 2, the delta(15)N values gradually decline, indicating complete cessation of breastfeeding by 3-4 years. Among adults, stature (males = 1.68 +/- 0.06 m; females = 1.58 +/- 0.07 m) and sexual dimorphism (106) were low, suggesting that the population was possibly under environmental stress. The delta(13)C results for adults are similar, but females show a small but statistically significantly (t = -2.86, P < 0.01) lower mean delta(15)N value (9.9 +/- 0.9 per thousand) compared to males (10.6 +/- 0.5 per thousand). These lower female delta(15)N values possibly reflect the different physiology of the sexes (pregnancy and/or lactation) or the reduced consumption of animal/fish protein by women, and this may have been influenced by individual preference, family needs, or societal values of the era. 相似文献
172.
The effects of lactate, acetate, glucose, insulin, starvation and alloxan-diabetes on protein synthesis in perfused rat hearts. 下载免费PDF全文
Compared with glucose, lactate + acetate stimulated ventricular protein synthesis in anterogradely perfused hearts from fed or 72 h-starved rats. Stimulation was greater on a percentage basis in starved rats. Atrial protein synthesis was not detectably stimulated by lactate + acetate. Insulin stimulated protein synthesis in atria and ventricles. The stimulation of protein synthesis by lactate + acetate and insulin was not additive, the percentage stimulation by insulin being less in the ventricles of lactate + acetate-perfused hearts than in glucose-perfused hearts. Perfusion of hearts from 72 h-starved or alloxan-diabetic rats with glucose + lactate + acetate + insulin did not increase protein-synthesis rates or efficiencies (protein synthesis expressed relative to total RNA) to values for fed rats, implying there is a decrease in translational activity in these hearts. In the perfused heart, inhibition of protein synthesis by starvation and its reversal by re-feeding followed a relatively prolonged time course. Synthesis was still decreasing after 3 days of starvation and did not return to normal until after 2 days of re-feeding. 相似文献
173.
The effect of dexamethasone on transferrin secretion by cultured fetal rat hepatocytes 总被引:1,自引:0,他引:1
Cultured fetal rat hepatocytes derived from 12, 15 and 19-day gestation rats are capable of secreting transferrin. When dexamethasone is added to the medium an increased secretion rate is observed. The changes in secretion rates in control as well as dexamethasone-treated cells during culture have been shown to correlate with the level of mRNA coding for transferrin. Immunocytochemical experiments show that initially all hepatocytes contain transferrin which is localized in the lumina of the perinuclear space, rough endoplasmic reticulum and in the saccules and vesicles of the Golgi apparatus. During culture, particularly in control cells, the intensity of labelling varies from cell to cell. In addition, adjacent cells are observed to label more intensely in different intracellular organelles. 相似文献
174.
How and When Selection Experiments Might Actually be Useful 总被引:2,自引:2,他引:2
Laboratory natural selection and artificial selection are vitaltools for addressing specific questions about evolutionary patternsof variation. Laboratory natural selection can illuminate whethera putative selective agent is capable of generating long-term,sustained changes in individual traits and suites of traits.Artificial selection is the essential tool for understandingthe general evolvability of traits and the extent to which geneticcorrelations constrain evolution. We review the contexts inwhich each type of experiment seems capable of offering keyinsights into important evolutionary issues. We also discusstheoretical and methodological considerations that play criticalroles in designing selection experiments that are relevant toevolutionary patterns of trait variation. In particular, wefocus on the critical role of selection intensity and the consequencesof experiments with different intensities. While selection experimentsare not practical in many cases, sophisticated selection experimentsdesignedwith careful consideration of the theory of selectionshouldbe taken beyond model organisms and used in well-chosen naturalsystems to understand natural patterns of variation. 相似文献
175.
176.
Capsule We report large declines among summer populations between 1968–80 and 2000. Aim To assess changes in the status of breeding populations of birds in pastoral uplands. Methods Volunteer observers revisited 13 areas of marginal upland in Britain where Common Birds Census data were collected during 1968–80. This allowed the status of 35 bird species to be examined over about 20 years and to make a comparison between grassland-based and woodland-based species. Results For 12 species the decline in abundance was significant, particularly among passerines, such as Skylark Alauda arvensis, Wheatear Oenanthe oenanthe, Whinchat Saxicola rubetra, Yellow Wagtail Motacilla flava and Yellowhammer Emberiza citrinella, each of which declined by over 80%. Redshank Tringa totanus, Yellow Wagtail, Dipper Cinclus cinclus, Whinchat, Wheatear, Ring Ouzel Turdus torquatus and Yellowhammer were found on less than half the number of plots on which they were originally recorded. Most declining species were associated with grassland for nesting and foraging, compared to those species that increased (Wood Pigeon Columba palumbus, Pied Wagtail Motacilla alba, Carrion Crow Corvus corone, Jackdaw C. monedula and Goldfinch Carduelis carduelis) that were less specialized in their habitat requirements. Woodland or woodland edge species showed no significant change in status, suggesting that population declines among grassland species were not due to lower observer effort between recording periods. Conclusion Long-term changes to grassland ecosystems in marginal upland areas of Britain may have influenced the status of bird populations. 相似文献
177.
Ethnographic inquiry in colonial India: Herbert Risley,William Crooke,and the study of tribes and castes 下载免费PDF全文
C.J. Fuller 《The journal of the Royal Anthropological Institute》2017,23(3):603-621
Sir Herbert Risley and William Crooke, both officials in the colonial government, published the first two handbooks of tribes and castes in British India in the 1890s, each containing a lengthy ethnographic glossary with entries for individual tribes and castes. The handbooks are rarely consulted by modern anthropologists of India and have been criticized as colonialist misrepresentation. This article, which reassesses Risley's and Crooke's handbooks as contributions to anthropological knowledge, examines their collection and presentation of ethnographic information, particularly Risley's inquiry into caste ranking. It discusses criticism of the handbooks and their elitist bias, as well as the collaborative contribution made by Indian assistants. It briefly considers why Risley's and Crooke's work was uninteresting to leading metropolitan anthropologists and notes the greater interest of European sociologists. 相似文献
178.
179.
Herpes simplex virus type 1 entry through a cascade of virus-cell interactions requires different roles of gD and gH in penetration. 总被引:3,自引:24,他引:3 下载免费PDF全文
We examined the entry process of herpes simplex virus type 1 (HSV-1) by using infectious virus and previously characterized noninfectious viruses that can bind to cells but cannot penetrate as a result of inactivation of essential viral glycoprotein D (gD) or H (gH). After contact of infectious virus with the cell plasma membrane, discernible changes of the envelope and tegument could be seen by electron microscopy. Noninfectious virions were arrested at distinct steps in interactions with cells. Viruses inactivated by anti-gD neutralizing antibodies attached to cells but were arrested prior to initiation of a visible fusion bridge between the virus and cell. As judged from its increased sensitivity to elution, virus lacking gD was less stably bound to cells than was virus containing gD. Moreover, soluble gD could substantially reduce virus attachment when added to cells prior to or with the addition of virus. Virus inactivated by anti-gH neutralizing antibodies attached and could form a fusion bridge but did not show expansion of the fusion bridge or extensive rearrangement of the envelope and tegument. We propose a model for infectious entry of HSV-1 by a series of interactions between the virion envelope and the cell plasma membrane that trigger virion disassembly, membrane fusion, and capsid penetration. In this entry process, gD mediates a stable attachment that is likely required for penetration, and gH seems to participate in fusion initiation or expansion. 相似文献
180.
Mazard SL Fuller NJ Orcutt KM Bridle O Scanlan DJ 《Applied and environmental microbiology》2004,70(12):7355-7364
An oligonucleotide primer, NITRO821R, targeting the 16S rRNA gene of unicellular cyanobacterial N2 fixers was developed based on newly derived sequences from Crocosphaera sp. strain WH 8501 and Cyanothece sp. strains WH 8902 and WH 8904 as well as several previously described sequences of Cyanothece sp. and sequences of intracellular cyanobacterial symbionts of the marine diatom Climacodium frauenfeldianum. This oligonucleotide is specific for the targeted organisms, which represent a well-defined phylogenetic lineage, and can detect as few as 50 cells in a standard PCR when it is used as a reverse primer together with the cyanobacterium- and plastid-specific forward primer CYA359F (U. Nubel, F. Garcia-Pichel, and G. Muyzer, Appl. Environ. Microbiol. 63:3327-3332, 1997). Use of this primer pair in the PCR allowed analysis of the distribution of marine unicellular cyanobacterial diazotrophs along a transect following the 67 degrees E meridian from Victoria, Seychelles, to Muscat, Oman (0.5 degrees S to 26 degrees N) in the Arabian Sea. These organisms were found to be preferentially located in warm (>29 degrees C) oligotrophic subsurface waters between 0 and 7 degrees N, but they were also found at a station north of Oman at 26 degrees N, 56 degrees 35'E, where similar water column conditions prevailed. Slightly cooler oligotrophic waters (<29 degrees C) did not contain these organisms or the numbers were considerably reduced, suggesting that temperature is a key factor in dictating the abundance of this unicellular cyanobacterial diazotroph lineage in marine environments. 相似文献