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51.
Charrel RN Feldmann H Fulhorst CF Khelifa R de Chesse R de Lamballerie X 《Biochemical and biophysical research communications》2002,295(5):1118-1124
Human thyroperoxidase (TPO) ectodomain is successively made of myeloperoxidase-, complement control protein repeat-, and epidermal growth factor-like gene modules. However, the TPO immunodominant region targeted by autoantibodies from patients with an autoimmune thyroid disease has not been mapped on the molecule. Here, we used two purified recombinant TPO peptides produced in eukaryotic cells, which correspond to the major first and the further two gene modules of TPO. We compared by ELISA their respective immunoreactivity with that of the recombinant soluble TPO containing all the three gene modules. We used well-characterized murine and human TPO monoclonal antibodies and human autoantibodies affinity-purified from a large pool of patients' sera. We found that the TPO immunodominant region was susceptible to denaturation and required the integrity of the molecule to be correctly expressed. We concluded that TPO B-cell autoepitopes are made by amino acids from the three gene modules, which fold in one highly conformational immunodominant region. 相似文献
52.
Molecular evolution of the period gene in Drosophila athabasca 总被引:1,自引:0,他引:1
We measured nucleotide variability within and between the three semispecies
of the Drosophila athabasca complex, at the period (per) gene by using a
polymerase chain reaction-based four-cutter restriction- enzyme analysis.
The levels of polymorphism varied considerably between the three
semispecies. Our results for per, combined with previous data for X-linked
allozymes, suggest that the X chromosome in the western- northern
semispecies is less variable than expected under an equilibrium-neutral
model. Both the pattern of divergence between the semispecies and a
cladistic clustering of per haplotypes support the previously hypothesized
grouping of eastern A and eastern B as the two most recently diverged
semispecies. A 21-bp in-frame segment in the region of per which shares
sequence similarity with the neuronal development gene single minded is
deleted in all eastern A and eastern B flies examined but is present in all
of the western-northern flies and all other published per sequences.
Despite these hints that there may be significant differences at the per
gene between the semispecies, especially the western-northern group versus
the two eastern groups, there is no compelling evidence that per is
involved in the mating song differences between the semispecies.
相似文献
53.
Avise JC; Shapira JF; Daniel SW; Aquadro CF; Lansman RA 《Molecular biology and evolution》1983,1(1):38-56
We address the problem of the possible significance of biological
speciation to the magnitude and pattern of divergence of asexually
transmitted characters in bisexual species. The empirical data for this
report consist of restriction endonuclease site variability in maternally
transmitted mitochondrial DNA (mtDNA) isolated from 82 samples of
Peromyscus polionotus and P. leucopus collected from major portions of the
respective species' ranges. Data are analyzed together with previously
published information on P. maniculatus, a sibling species to polionotus.
Maps of restriction sites indicate that all of the variation observed can
be reasonably attributed to base substitutions leading to loss or gain of
particular recognition sites. Magnitude of mtDNA sequence divergence within
polionotus (maximum approximately equal to 2%) is roughly comparable to
that observed within any of five previously identified mtDNA assemblages in
maniculatus. Sequence divergence within leucopus (maximum approximately
equal to 4%) is somewhat greater than that within polionotus. Consideration
of probable evolutionary links among mtDNA restriction site maps allowed
estimation of matriarchal phylogenies within polionotus and leucopus.
Clustering algorithms and qualitative Wagner procedures were used to
generate phenograms and parsimony networks, respectively, for the
between-species comparisons. Three simple graphical models are presented to
illustrate some conceivable relationships of mtDNA differentiation to
speciation. In theoretical case I, each of two reproductively defined
species (A and B) is monophyletic in matriarchal genealogy; the common
female ancestor of either species can either predate or postdate the
speciation. In case II, neither species is monophyletic in matriarchal
genotype. In case III, species B is monophyletic but forms a subclade
within A which is thus paraphyletic with respect to B. The empirical
results for mtDNA in maniculatus and polionotus appear to conform closely
to case III. These theoretical and empirical considerations raise a number
of questions about the general relationship of the speciation process to
the evolution of uniparentally transmitted traits. Some of these
considerations are presented, and it is suggested that the distribution
patterns of mtDNA sequence variation within and among extant species should
be of considerable relevance to the particular demographies of speciation.
相似文献
54.
Debora Napoli Leonardo Lupori Raffaele Mazziotti Giulia Sagona Sara Bagnoli Muntaha Samad Erika K Sacramento Joanna Kirkpartick Elena Putignano Siwei Chen Eva Terzibasi Tozzini Paola Tognini Pierre Baldi Jessica CF Kwok Alessandro Cellerino Tommaso Pizzorusso 《EMBO reports》2021,22(1)
The authors regret having omitted grant attributions in the original publication. The funding section is herewith updated to reflect the change. “Funding attributed to Tommaso Pizzorusso was provided by EPIGEN Flagship project and PRIN2017HM8FA, funding attributed to Alessandro Cellerino was provided by Fondazione Pisa ETHERNA project, funding attributed to Pierre Baldi was provided by NIH (grant NIH ), funding attributed to Jessica Kwok was provided by the Leverhulme Trust project grant (RPG‐2018‐100).” GM123558相似文献