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91.
92.
Double-Stranded RNA in Rice 总被引:2,自引:0,他引:2
Toshiyuki Fukuhara 《Journal of plant research》1999,112(1):131-138
Oryza sativa ) and wild rice (O. rufipogon) tissues. It is detected at every developmental stage, and is transmitted very efficiently to progeny via seeds (more than
98%). The dsRNA is maintained at a constant level (approximately 100 copies/cell) in almost all tissues. However, the number
of copies increases about 10-fold when host cells are grown in suspension culture. Complete nucleotide sequences of cultivated
rice (temperate japonica rice, cv. Nipponbare, J-dsRNA) and wild rice (W-1714, W-dsRNA) dsRNAs have been determined. Both wild and cultivated rice
dsRNAs have a single long open reading frame (ORF) containing the conserved motifs of RNA-dependent RNA polymerase and RNA
helicase. The coding strands of both contain a site-specific discontinuity (nick) at nt 1,211 (J-dsRNA) or at nt 1,197 (W-dsRNA)
from the 5′ end of their coding strand. Rice dsRNA has several unique properties and can be regarded as a novel RNA replicon.
This paper discusses the origin and evolution of the rice dsRNA.
Received 23 October 1998/ Accepted in revised form 15 December 1998 相似文献
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Continuously applied compressive pressure induces bone resorption by a mechanism involving prostaglandin E2 synthesis 总被引:4,自引:0,他引:4
K Imamura H Ozawa T Hiraide N Takahashi Y Shibasaki T Fukuhara T Suda 《Journal of cellular physiology》1990,144(2):222-228
In previous research, we devised a specific culture chamber to examine the effect of continuously applied compressive pressure (CCP) on bone formation and resorption. The chamber was infused with compressed mixed gases with different O2 and CO2 composition to maintain the pO2, pCO2, and pH in the culture medium under pressures of +0.5 atm (1.5 atm total) to +2.0 atm (3.0 atm total) at the same levels as those at the ordinary pressure (1 atm). Using the specific culture chamber, we demonstrated that CCP greatly suppressed the differentiation of mouse osteoblast-like MC3T3-E1 cells. The inhibition by CCP appeared to be mediated by prostaglandin E2 (PGE2). In the present study, we examined the effect of CCP on osteoclastic bone resorption. CCP treatment of mouse bone marrow culture markedly increased both the PGE2 production and the number of tartrate-resistant acid phosphatase (TRACP)-positive mononuclear cells (possibly precursors of multinucleated osteoclasts). An autoradiographic study using [125I]-salmon calcitonin showed clearly that those TRACP-positive cells had calcitonin receptors. The CCP effect was the greatest at +1.0 atm (2.0 atm total). Isobutylmethylxanthine potentiated the production of TRACP-positive cells induced by CCP. Adding indomethacin completely inhibited both the TRACP-positive cell formation and the PGE2 production induced by CCP. CCP also increased the release of 45Ca from prelabeled mouse calvaria during later stages (2-6 days) of the 6-day culture period. CCP markedly increased PGE2 but not interleukin 1 in the culture media of mouse calvaria. These results indicate that, besides inhibiting osteoblast differentiation, CCP stimulates bone resorption by generating new osteoclasts through a mechanism involving PGE2 production. 相似文献
95.
Yoichiro Fujioka Shinya Nishide Toyoyuki Ose Tadaki Suzuki Izumi Kato Hideo Fukuhara Mari Fujioka Kosui Horiuchi Aya O. Satoh Prabha Nepal Sayaka Kashiwagi Jing Wang Mika Horiguchi Yuko Sato Sarad Paudel Asuka Nanbo Tadaaki Miyazaki Hideki Hasegawa Yusuke Ohba 《Cell host & microbe》2018,23(6):809-818.e5
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Yuki Nakayama Akiko Yamamoto Masaki Tanaka Atsunori Fukuhara Iichiro Shimomura 《Biochemical and biophysical research communications》2009,379(2):288-292
Rho GTPase regulates actin cytoskeleton organization and assembly in many cell types, however, its significance in adipose tissue is not well characterized. Here, we demonstrate high RhoA activity in adipose tissues of C57BL/6J mice. To determine the effect of RhoA activation on 3T3-L1 cells, stable cell lines overexpressing G14VRhoA fused to destabilizing domain of FKBP12 (DD-G14VRhoA-L1) were generated. Treatment of DD-G14VRhoA-L1 cells with Shield1 following their differentiation into adipocytes, resulted in the appearance of thick cortical actin filaments, and increased the mRNA expression levels of plasminogen activator inhibitor type-1 (PAI-1) and monocyte chemoattractant protein-1 (MCP-1). The induction of PAI-1 and MCP-1 was inhibited by treatment with a Rho-associated kinase (ROCK) inhibitor, Y-27632. In 3T3-L1 adipocytes, tumor necrosis factor-α activated RhoA and increased mRNA expression of PAI-1 and MCP-1, and their treatment with Y-27632 partially inhibited these changes. The results indicate that RhoA-ROCK pathway induces inflammatory cytokine expression in adipocytes. 相似文献