The Involvement of Indole-3-Acetic Acid Produced by Bradyrhizobium elkanii in Nodule Formation

IAA-deficient mutants T10, T27 and TN3 were isolated from B.elkanii USDA 31 by spontaneous and/or N-methyl-N'-nitro-N-nitrosoguanidine(NTG) mutageneses. Inoculation with these mutants significantlyreduced the nodule number on soybean roots when compared tothat of the parent strain. Furthermore, exogenous IAA applicationrestored the nodule number of soybeans inoculated with TN3 tothe original level. (Received July 18, 1994; Accepted September 13, 1994)     

The VIG9 gene products from the human pathogenic fungi Candida albicans and Candida glabrata encode GDP-mannose pyrophosphorylase

We have identified two genomic DNA fragments from the human pathogenic fungi, Candida albicans (CaVIG9) and Candida glabrata (CgVIG9) that encode GDP-mannose pyrophosphorylase, a key enzyme for protein glycosylation. The VIG9 homologues of CaVIG9 and CgVIG9 complement an identified protein glycosylation-defective mutation, vig9, of Saccharomyces cerevisiae. The nucleotide sequences of the ORFs, which are 83 and 90% identical to that of the ScVIG9 protein, respectively, showed a predicted gene product homologous to S. cerevisiae GDP-mannose pyrophosphorylase. We examined the enzyme activity of a glutathione S-transferase fusion of each VIG9 gene to synthesize GDP mannose in the cell extracts of a heterologous Escherichia coli expression system. We also developed a method for detecting the enzyme activity using a non-radioactive substrate that would be applicable to high throughput screening.     

Double-stranded RNA-binding protein DRB3 negatively regulates anthocyanin biosynthesis by modulating PAP1 expression in Arabidopsis thaliana

Journal of Plant Research - The model plant Arabidopsis thaliana has five double-stranded RNA-binding proteins (DRB1-DRB5), two of which, DRB1 and DRB4, are well characterized. In contrast, the...