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141.
The performance of diagnostic cytology on Papanicolaou smears can be periodically monitored by calculating the total discrimination or the total divergence of the cytologic diagnoses against the histologic diagnoses on samples obtained by colposcope-directed biopsies. Using these measures, the annual performances of the Gynecologic Cytology Laboratory of the University of Minnesota between 1980 and 1988 were retrospectively analyzed. For those years, the total discrimination and total divergence behaved similarly and were sensitive to the performance of the total system, including specimen sampling errors and laboratory precision. The lowest limits of the permissible range of the total discrimination and total divergence were 0.15 and -1.21 decits, respectively, for a single-slide Papanicolaou test if an 80% "hit" rate was accepted as the lowest threshold for each category. The optimal numbers of category-states were not a sensitive indicator of the quality of a laboratory; i.e., the optimal number of diagnostic categories remained at three throughout the period studied. 相似文献
142.
F K Fujimura 《Nucleic acids research》1988,16(5):1987-1997
A point mutation in the enhancer of polyomavirus host range mutant, PyEC F441, permits productive infection of the murine embryonal carcinoma cell line, F9. This mutation at nucleotide position 5258 introduces a local conformational change in naked viral DNA. The effect of all four possible nucleotide sequences at position 5258 on local DNA conformation was analyzed by gel electrophoresis of fragments produced by ligation of synthetic oligonucleotides having these sequences. The results indicated that both the wild-type and the F441 sequences introduced local structural polymorphism that can lead to DNA bending. The wild-type sequence had a greater effect on DNA curvature than the F441 sequence. The two other sequences at nucleotide 5258 did not appear to introduce detectable amounts of DNA curvature. 相似文献
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S Nishikawa J Adiwinata H Morioka T Fujimura T Tanaka S Uesugi T Hakoshima K Tomita S Nakagawa M Ikehara 《Protein engineering》1990,3(5):443-448
Molecular-dynamic calculations predict that, if Tyr24 and Asn84 are each replaced by a Cys residue, it should be possible to form a third disulfide bond in ribonuclease T1 (RNase T1) between these residues, with only minimal conformational changes at the catalytic site. The gene encoding such a mutant variant of RNase T1 (Tyr24----Cys24, Asn84----Cys84) was constructed by the cassette mutagenesis method using a chemically synthesized gene. In order to reduce the toxic effect of the mutant enzyme (RNase T1S) on an Escherichia coli host, we arranged for the protein to be secreted into the periplasmic space by using a vector that harbors a gene for an alkaline phosphatase signal peptide under the control of the trp promoter. The nucleolytic activity of RNase T1S toward pGpC was approximately the same as that of RNase T1 at 37 degrees C (pH 7.5). Moreover, at 55 degrees C, RNase T1S retained nearly 70% of its activity while the activity of the wild-type enzyme was reduced to less than 10%. RNase T1S was also more resistant to denaturation by urea than the wild-type enzyme. However, unlike RNase T1, RNase T1S was irreversibly and almost totally inactivated by boiling at 100 degrees C for 15 min. 相似文献
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A novel double assembly was prepared by association between a columnar molecular assembly of cyclic tri-beta-peptides having sugar units and lectins. The NMR, FT-IR, and circular dichroism (CD) spectroscopy as well as computational calculations revealed that this compound took a flat and C3 symmetrical conformation and that the amide N-H and C=O groups protruded vertically to the ring plane. This disk-shaped molecule stacked one by one to form a columnar structure via intermolecular hydrogen bonds between the amide groups. WGA lectin moderately bound to this columnar assembly to form a double assembly. Another lectin (Con A) disturbed the columnar structure upon strong binding, and RCA lectin showed no binding. Fluorescence spectroscopy revealed that the association between WGA lectin and columnar assembly of cyclic glycopeptide could be achieved due to the high density of the hydroxyl groups on the assembly surface (cluster effects). Interestingly, after cross-linking the lectins bound to the columnar assembly (the double assembly) by glutaraldehyde, the core column of cyclic tri-beta-peptides could be washed away to leave the protein nanotube. 相似文献
149.
R Fujimura Y Sato T Nishizawa K Oshima SW Kim M Hattori T Kamijo H Ohta 《Journal of bacteriology》2012,194(15):4122-4123
A diazotrophic, acidophilic, iron-oxidizing bacterium, Leptospirillum ferrooxidans, known to be difficult to cultivate, was isolated from a fresh volcanic ash deposit on the island of Miyake, Japan. Here, we report the complete genome sequence of a cultured strain, C2-3. 相似文献