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961.
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A case of Cushing's syndrome due to huge adrenocortial multinodular hyperplasia who was shown to be hyperresponsive to ACTH administration, unresponsive to metyrapone administration and resistant to dexamethasone high dose suppression was reported. After two years' duration of his symptoms, the multinodular adrenals weighing 161 g in total were removed by bilateral adrenalectomy which abolished his symptoms. Postoperatively, plasma ACTH rose gradually to above normal levels, suggesting the presence of primary disorder in the hypothalamus-pituitary axis. 相似文献
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Superoxide (O2-)-generating membranes of pig blood neutrophils were studied by the ESR spin-label method. Neutrophils were spin-labeled with doxylstearic acids, consisting of nitroxide free radicals bonded to the 5, 7, 12, or 16 position of stearic acid (5-, 7-, 12-, or 16-DS), to detect the reduction of their nitroxide radicals at different positions in the membrane. The spin-labeled cells were then stimulated with phorbol myristate acetate (PMA). Stimulation of the labeled cells resulted in a marked decrease in the spin concentration of 5-DS due to the reduction by O2-, but not in those of the other three DS labels. This reduction of 5-DS was completely inhibited by copper salicylate (CS), a hydrophobic and permeable O2(-)-scavenger, but not by superoxide dismutase (SOD). CS was not inhibitory on the respiratory burst, i.e., O2(-)-generating activity of neutrophils. On the contrary, if the spin-labels were present in the extracellular medium, SOD inhibited the reduction of all four DS labels due to O2- released from PMA-stimulated cells. These results suggest that the O2(-)-releasing site is not located at the outer surface of the plasma membrane but in an inner hydrophobic environment a short distance (around 4-5 A) from its outer surface. 相似文献
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Takagi Michio T.sriwong Kotchakorn Masuda Ayaka Kawaguchi Nozomi Fukui Shusuke Le Viet Lan Huong Kato Dai-ichiro Kitayama Takashi Fujii Mikio Koesoema Afifa Ayu Matsuda Tomoko 《Biotechnology letters》2022,44(3):461-471
Biotechnology Letters - A novel biocatalyst for Baeyer–Villiger oxidations is necessary for pharmaceutical and chemical industries, so this study aims to find a Baeyer–Villiger... 相似文献
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Hanae Izu Mayumi Okuda Sachi Shibata Tsutomu Fujii Kiminori Matsubara 《Bioscience, biotechnology, and biochemistry》2019,83(4):747-750
Six-week-old male KK-Ay mice received drinking water with S-adenosylmethionine (SAM), α-glycerophosphocholine (GPC), or SAM+GPC for 10 weeks. The serum glucose of SAM+GPC at 15 weeks old, total cholesterol of GPC at 12 weeks old, and triglyceride of GPC at 15 weeks old and of SAM at 16 weeks old were reduced. SAM+GPC reduced serum leptin and food intake.
Abbreviations: SAM: S-adenosylmethionine; GPC: α-glycerophosphocholine 相似文献