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81.
Escherichia coli is a valuable commercial host for the production of heterologous proteins. We used elementary mode analysis to identify all
possible genetically independent pathways for the production of three specific recombinant proteins, green fluorescent protein,
savinase and an artificial protein consisting of repeating units of a five-amino-acid cassette. Analysis of these pathways
led to the identification of the most efficient pathways for the production of each of these proteins. The results indicate
that the amino acid composition of expressed proteins has a profound effect on the number and identity of possible pathways
for the production of these proteins. We show that several groups of elementary modes produce the same ratio of biomass and
recombinant protein. The pattern of occurrence of these modes is dependent on the amino acid composition of the specific foreign
protein produced. These pathways are formed as systemic combinations of other pathways that produce biomass or foreign protein
alone after the elimination of fluxes in specific internal reversible reactions or the reversible carbon dioxide exchange
reaction. Since these modes represent pathway options that enable the cell to produce biomass and protein without utilizing
these reactions, removal of these reactions would constrain the cells to utilize these modes for producing biomass and foreign
protein at constant ratios. 相似文献
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83.
Although synaptic functions of ionotropic glutamate receptors in the olfactory bulb have been studied in vitro, their roles in pattern processing in the intact system remain controversial. We therefore examined the functions of ionotropic glutamate receptors during odor processing in the intact olfactory bulb of zebrafish using pharmacological manipulations. Odor responses of mitral cells and interneurons were recorded by electrophysiology and 2-photon Ca(2+) imaging. The combined blockade of AMPA/kainate and NMDA receptors abolished odor-evoked excitation of mitral cells. The blockade of AMPA/kainate receptors alone, in contrast, increased the mean response of mitral cells and decreased the mean response of interneurons. The blockade of NMDA receptors caused little or no change in the mean responses of mitral cells and interneurons. However, antagonists of both receptor types had diverse effects on the magnitude and time course of individual mitral cell and interneuron responses and, thus, changed spatio-temporal activity patterns across neuronal populations. Oscillatory synchronization was abolished or reduced by AMPA/kainate and NMDA receptor antagonists, respectively. These results indicate that (1) interneuron responses depend mainly on AMPA/kainate receptor input during an odor response, (2) interactions among mitral cells and interneurons regulate the total olfactory bulb output activity, (3) AMPA/kainate receptors participate in the synchronization of odor-dependent neuronal ensembles, and (4) ionotropic glutamate receptor-containing synaptic circuits shape odor-specific patterns of olfactory bulb output activity. These mechanisms are likely to be important for the processing of odor-encoding activity patterns in the olfactory bulb. 相似文献
84.
Weidler GW Dornmayr-Pfaffenhuemer M Gerbl FW Heinen W Stan-Lotter H 《Applied and environmental microbiology》2007,73(1):259-270
Scanning electron microscopy revealed great morphological diversity in biofilms from several largely unexplored subterranean thermal Alpine springs, which contain radium 226 and radon 222. A culture-independent molecular analysis of microbial communities on rocks and in the water of one spring, the "Franz-Josef-Quelle" in Bad Gastein, Austria, was performed. Four hundred fifteen clones were analyzed. One hundred thirty-two sequences were affiliated with 14 bacterial operational taxonomic units (OTUs) and 283 with four archaeal OTUs. Rarefaction analysis indicated a high diversity of bacterial sequences, while archaeal sequences were less diverse. The majority of the cloned archaeal 16S rRNA gene sequences belonged to the soil-freshwater-subsurface (1.1b) crenarchaeotic group; other representatives belonged to the freshwater-wastewater-soil (1.3b) group, except one clone, which was related to a group of uncultivated Euryarchaeota. These findings support recent reports that Crenarchaeota are not restricted to high-temperature environments. Most of the bacterial sequences were related to the Proteobacteria (alpha, beta, gamma, and delta), Bacteroidetes, and Planctomycetes. One OTU was allied with Nitrospina sp. (delta-Proteobacteria) and three others grouped with Nitrospira. Statistical analyses suggested high diversity based on 16S rRNA gene analyses; the rarefaction plot of archaeal clones showed a plateau. Since Crenarchaeota have been implicated recently in the nitrogen cycle, the spring environment was probed for the presence of the ammonia monooxygenase subunit A (amoA) gene. Sequences were obtained which were related to crenarchaeotic amoA genes from marine and soil habitats. The data suggested that nitrification processes are occurring in the subterranean environment and that ammonia may possibly be an energy source for the resident communities. 相似文献
85.
86.
Friedrich Boas 《Protoplasma》1930,9(1):428-440
Zusammenfassung Schon sehr kleine Mengen von Gallensalzen, wie desoxycholsaures und apocholsaures Natrium lassen aus Zellen Stoffe austreten, ohne da\ die Zellen tot sind. Die ausgetretenen Stoffe können mit der Schüttelprobe (Oberflächenspannung), mit der Ninhydrinprobe (Eiwei\bruchstücke), und mit der Silbernitratprobe erfa\t werden.Auch kleine Mengen von Quecksilberchlorid veranlassen Austritt von Stoffen aus der Zelle. Dabei sind die Zellen nicht tot. Bei den Gallensalzproben ist eine untere Grenze der Wirksamkeit schwer anzugeben. Wenn nicht gerade (bei Kartoffelversuchen) Randstücke oder Siebteile mit viel Eiwei\ vorliegen, ist eine Normalgrenze der Wirkung etwa bei 1 75000 bis 1 100000 erreicht, soweit die Ninhydrinprobe in Frage kommt. Bei Gegenwart eiwei\reicher Kartoffelzellen bzw. Siebteile ist die Grenze der mit Ninhydrin zu erfassenden Wirkung viel tiefer anzusetzen, etwa 1 1000000. Durch die physiologischen oder anatomisch-topographischen Sondereigenschaften (viel Eiwei\, viel Siebteile in einzelnen Proben) erhält die Gallensalzmethode bis zu einem gewissen Grad etwas Subjektives. Trotzdem steht unter Umständen der Gallensalzeinflu\ 1 1000000 au\er jedem Zweifel! Die Methodik setzt eben eine gewisse Erfahrung voraus.Bei höheren Pflanzen kann mit der Neutralsalz-Gallensalzbehandlung der Unterschied zwischen Epidermiszellen und Spaltöffnungsapparat ungemein scharf vordemonstriert werden. Bei geeigneter Behandlung lösen sich Zellkerne schrittweise in Neutralsalz-Gallensalz auf, die Kerne der Spaltöffnungszellen sind resistenter. 相似文献
87.
Summary A girl with partial deletion of the short arms of one chromosome 7 is described. Among many other symptoms she has craniosynostosis. Early closure of cranio-sutures has previously been described in 2 of 3 patients with partial deletion 7. Investigation of a number of genetic marker systems shows that the HL-A, MN, AcP, and GPT loci are not located in the deleted segment.
Zusammenfassung Es wird ein Mädchen mit teilweiser Deletion des kurzen Armes eines Chromosoms 7 beschrieben. Außer vielen anderen Symptomen hat sie eine Craniosynostose. Frühzeitiger Verschluß der Schädelnähte wurde auch bei 2 von 3 Patienten mit Deletion 7, die in der Literatur beschrieben sind, beobachtet. Untersuchung einer Reihe genetischer Markersysteme zeigt, daß die HL-A-, MN-, AcP- und GPT-loci nicht in dem deletierten Segment liegen.相似文献
88.
89.
Hermann Cuntz Friedrich Forstner Bettina Schnell Georg Ammer Shamprasad Varija Raghu Alexander Borst 《PloS one》2013,8(8)
Important brain functions need to be conserved throughout organisms of extremely varying sizes. Here we study the scaling properties of an essential component of computation in the brain: the single neuron. We compare morphology and signal propagation of a uniquely identifiable interneuron, the HS cell, in the blowfly (Calliphora) with its exact counterpart in the fruit fly (Drosophila) which is about four times smaller in each dimension. Anatomical features of the HS cell scale isometrically and minimise wiring costs but, by themselves, do not scale to preserve the electrotonic behaviour. However, the membrane properties are set to conserve dendritic as well as axonal delays and attenuation as well as dendritic integration of visual information. In conclusion, the electrotonic structure of a neuron, the HS cell in this case, is surprisingly stable over a wide range of morphological scales. 相似文献
90.