In vitro incorporation of 2′-deoxyadenosine and 3′-deoxyadenosine into yeast tRNAPhe using tRNA nucleotidyl transferase,and properties of tRNAPhe-C-C-2′dA and tRNAPhe-C-C-3′dA

2′-Deoxyadenosine and 3′-deoxyadenosine (cordycepin) can be incorporated into the 3′-terminal position of tRNA^Phe by tRNA nucleotidyl transferase. tRNA^Phe-C-C-2′dA and tRNA^Phe-C-C-3′dA, missing the cis-diol group at the 3′-terminal end are resistant to periodate oxidation and are not able to form borate complexes. In aminoacylation experiments only the tRNA^Phe-C-C-3′dA proved to be chargeable.     

Esterase-2 in Ephestia kühniella. II. Tissue-specific patterns

Esterase-2 polymorphism has been investigated quantitatively. Staining intensities of the homodimer bands mm, ff, and ss are not equally expressed but found in relative activities of 1:0.5:0.7 in fat bodies and 1:1:3 in testes and gut walls. Relative intensities of the parental bands are binomially distributed in the three-banded patterns of heterozygotes in an exactly tissue-specific manner. Organ-specific proportions of relative activity remain constant throughout postembryonic development. Among reasons which may influence genesis of allele- and organ-specific activities of homodimer bands in homozygotes and may lead to asymmetrical distribution of intensities in the patterns of heterozygotes, a hypothesis of differential allelic activity is discussed, according to which the structural alleles 2 ^m , 2 ^f , and 2 ^s are closely linked to the alleles RG ^a , RG ^b , and RG ^c of a controlling gene.This work was supported by the Deutsche Forschungsgemeinschaft and submitted with Part I in partial fulfillment of the requirements for the degree of Dr.-habil. at the University of Tübingen.     

Book reviews

Ohne Zusammenfassung

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Book reviews

     

Chromosome Abnormalities in Patients Treated with Chlorpromazine,Perphenazine, and Lysergide

In a chromosome study on leucocyte cultures made in 13 patients treated with chlorpromazine, 15 treated with perphenazine, and nine treated with lysergide, a significantly higher frequency of gaps, breaks, and hypodiploid cells in the patients treated with perphenazine and lysergide occurred compared with the 41 controls studied. It is concluded that if some drugs can induce major chromosome abnormalities, and less toxic alternatives are available, the latter should be used in preference.     

Chromosomenabnormitäten und Behandlung mit Imuran® (Azathioprin) nach Nierentransplantationen

Zusammenfassung 19 Patienten, die nach einer Nierentransplantation mit Imuran behandelt wurden, wiesen eine erhöhte Anzahl Chromosomenabnormitäten im Vergleich zu 30 gesunden Kontrollpersonen der gleichen Altersklasse auf. Keine signifikant erhöhte Anzahl an Chromosomenabnormitäten fand sich jedoch bei 7 Patienten, die vor und unter der Imuran-behandlung untersucht wurden. Es wird vermutet, daß die Chromosomenabnormitäten bei den 19 mit Imuran behandelten Patienten wahrscheinlich nicht durch Imuran, sondern durch die Urämie entstanden sind.

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Summary 19 patients treated with Imuran after a renal transplantation showed a high frequency of chromosome abnormalities, compared with 30 healthy control persons in the same age range. No significantly higher frequency of chromosome abnormalities was, however, found in 7 patients examined before and during treatment with Imuran. It is suggested that the chromosome abnormalities found in the 19 patients treated with Imuran were probably not due to Imuran but to Uremia.

     

Ein atlas der spaltsinnesorgane von Cupiennius salei keys. Chelicerata (Araneae)

To facilitate further physiological investigation, a survey was undertaken of all the slit sense organs to be found on the body of the spider Cupiennius salei. We counted and mapped more than 3 000 sensory slits in the cuticle about half of which are combined to small groups of up to 29 slits forming compound or lyriform organs.     

Gerbstoffproduktion der Calluskulturen von Juniperus communis L.

Zusammenfassung Bestimmungen des Gerbstoffgehalts in vitro kultivierter Calli von Juniperus communis L. innerhalb einer Kulturperiode von 90 Tagen ergaben ein Maximum zu Beginn der Phase stärkeren Wachstums, etwa 30 Tage nach Versuchsbeginn.Durch Veränderungen der Glucosekonzentration des Substrates wird mit der Gerbstoffproduktion gleichzeitig auch das Wachstum der Calli beeinflußt. Deshalb wird eine deutliche Zunahme des Gerbstoffgehalts erst bei einer Steigerung der Kohlenhydratkonzentration von 1 auf 6% sichtbar.

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Tannin production in the Callus cultures of Juniperus Communis L.

Summary Callus cultures of Juniperus communis L. were analysed for tannins at intervals during a period of 90 days. The tannin content (mg tannins x 100/mg dry weight) reached a maximum at the onset of rapid growth, 30 days after inoculation, and then decreased. Variation of the glucose concentration in the medium influences both the growth and the production of tannins. An increase of the tannin content can be observed only if the carbohydrate concentration is raised from 1 to 6%.

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Herrn Prof. Dr. R. Harder zum 80. Geburtstag gewidmet.