全文获取类型
收费全文 | 141篇 |
免费 | 8篇 |
国内免费 | 1篇 |
专业分类
150篇 |
出版年
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 5篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 7篇 |
2017年 | 3篇 |
2016年 | 1篇 |
2015年 | 6篇 |
2014年 | 5篇 |
2013年 | 6篇 |
2012年 | 11篇 |
2011年 | 6篇 |
2010年 | 7篇 |
2009年 | 9篇 |
2008年 | 7篇 |
2007年 | 6篇 |
2006年 | 4篇 |
2005年 | 1篇 |
2004年 | 2篇 |
2003年 | 4篇 |
2001年 | 2篇 |
1999年 | 4篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 3篇 |
1971年 | 2篇 |
1968年 | 1篇 |
排序方式: 共有150条查询结果,搜索用时 15 毫秒
111.
112.
Jurriaan J H?lzenspies Willem Stoorvogel Ben Colenbrander Bernard AJ Roelen Dagmar R Gutknecht Theo van Haeften 《BMC developmental biology》2009,9(1):8
Background
Mammalian oocytes acquire competence to be fertilized during meiotic maturation. The protein kinase CDC2 plays a pivotal role in several key maturation events, in part through controlled changes in CDC2 localization. Although CDC2 is involved in initiation of maturation, a detailed analysis of CDC2 localization at the onset of maturation is lacking. In this study, the subcellular distribution of CDC2 and its regulatory proteins cyclin B and SPDY in combination with several organelle markers at the onset of pig oocyte maturation has been investigated. 相似文献113.
Gwendoline Kint Kathleen AJ Sonck Geert Schoofs David De Coster Jos Vanderleyden Sigrid CJ De Keersmaecker 《BMC microbiology》2009,9(1):198
Background
Quorum sensing is a term describing a bacterial communication system mediated by the production and recognition of small signaling molecules. The LuxS enzyme, catalyzing the synthesis of AI-2, is conserved in a wide diversity of bacteria. AI-2 has therefore been suggested as an interspecies quorum sensing signal. To investigate the role of endogenous AI-2 in protein expression of the Gram-negative pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), we performed a 2D-DIGE proteomics experiment comparing total protein extract of wildtype S. Typhimurium with that of a luxS mutant, unable to produce AI-2. 相似文献114.
William J Holmes Richard AJ Darby Martin DB Wilks Rodney Smith Roslyn M Bill 《Microbial cell factories》2009,8(1):35-14
Background
The optimisation and scale-up of process conditions leading to high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences. Typical experiments rely on varying selected parameters through repeated rounds of trial-and-error optimisation. To rationalise this, several groups have recently adopted the 'design of experiments' (DoE) approach frequently used in industry. Studies have focused on parameters such as medium composition, nutrient feed rates and induction of expression in shake flasks or bioreactors, as well as oxygen transfer rates in micro-well plates. In this study we wanted to generate a predictive model that described small-scale screens and to test its scalability to bioreactors. 相似文献115.
The availability of genomic and proteomic data from across the tree of life has made it possible to infer features of the genome and proteome of the last universal common ancestor (LUCA). A number of studies have done so, all using a unique set of methods and bioinformatics databases. Here, we compare predictions across eight such studies and measure both their agreement with one another and with the consensus predictions among them. We find that some LUCA genome studies show a strong agreement with the consensus predictions of the others, but that no individual study shares a high or even moderate degree of similarity with any other individual study. From these observations, we conclude that the consensus among studies provides a more accurate depiction of the core proteome of the LUCA and its functional repertoire. The set of consensus LUCA protein family predictions between all of these studies portrays a LUCA genome that, at minimum, encoded functions related to protein synthesis, amino acid metabolism, nucleotide metabolism, and the use of common, nucleotide‐derived organic cofactors. 相似文献
116.
van de Loo FA 《Arthritis research & therapy》2011,13(2):111
It has long been recognized that laboratory tests are useful in the diagnosis of disease and to monitor treatment outcome.
Their performance has become even more demanding with the development of personalized medicine. In patients with rheumatoid
arthritis (RA) the standard biochemical tests measure serological markers of disease, such as C-reactive protein, and RA-associated
auto-antibodies, such as rheumatoid factor and anti-citrullinated protein antibodies. The information obtained from these
markers does not, however, provide a complete picture of the disease and treatment efficacy. New biomarkers based on cytokine
receptor complexes are promising for RA theragnostics. 相似文献
117.
We sequenced the entire control region and portions of flanking genes
(tRNA(Phe), tRNA(Glu), and ND6) in the common chaffinch (Fringilla
coelebs), blue chaffinch (F. teydea), brambling (F. montifringilla), and
greenfinch (Carduelis chloris). In these finches the control region is
similar in length (1,223-1,237 bp) and has the same flanking gene order as
in other birds, and contains a putative TAS element and the highly
conserved CSB-1 and F, D, and C boxes recognizable in most vertebrates.
Cloverleaf-like structures associated with the TAS element at the 5' end
and CSB-1 at the 3' end of the control region may be involved with the stop
and start of D-loop synthesis, respectively. The pattern of nucleotide and
substitution bias is similar to that in other vertebrates, and consequently
the finch control region can be subdivided into a central, conserved G-rich
domain (domain II) flanked by hypervariable 5'-C-rich (domain I) and
3'-AT-rich (domain III) segments. In pairwise comparisons among finch
species, the central domain has unusually low transition/transversion
ratios, which suggests that increased G + T content is a functional
constraint, possibly for DNA primase efficiency. In finches the relative
rates of evolution vary among domains according to a ratio of 4.2 (domain
III) to 2.2 (domain I) to 1 (domain II), and extensively among sites within
domains I and II. Domain I and III sequences are extremely useful in
recovering intraspecific phylogeographic splits between populations in
Africa and Europe, Madeira, and a basal lineage in Nefza, Tunisia. Domain
II sequences are highly conserved, and are therefore only useful in
conjunction with sequences from domains I and III in phylogenetic studies
of closely related species.
相似文献
118.
119.
Relocation of a Ca2+-dependent protein kinase activity during pollen tube reorientation 总被引:9,自引:0,他引:9
下载免费PDF全文
![点击此处可从《The Plant cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Pollen tube reorientation is a dynamic cellular event that is crucial for successful fertilization. We have shown previously that pollen tube orientation is regulated by cytosolic free calcium ([Ca2+]c). In this paper, we studied the activity of a Ca2+-dependent protein kinase during reorientation. The kinase activity was assayed in living cells by using confocal ratio imaging of BODIPY FL bisindolylmaleimide. We found that growing pollen tubes exhibited higher protein kinase activity in the apical region, whereas nongrowing cells showed uniform distribution. Modification of growth direction by diffusion of inhibitors/activators from a micropipette showed the spatial redistribution of kinase activity to predict the new growth orientation. Localized increases in [Ca2+]c induced by photolysis of caged Ca2+ that led to reorientation also increased kinase activity. Molecular and immunological assays suggest that this kinase may show some functional homology with protein kinase C. We suggest that the tip-localized gradient of kinase activity promotes Ca2+-mediated exocytosis and may act to regulate Ca2+ channel activity. 相似文献
120.