全文获取类型
收费全文 | 1156篇 |
免费 | 82篇 |
出版年
2023年 | 2篇 |
2022年 | 9篇 |
2021年 | 21篇 |
2020年 | 9篇 |
2019年 | 16篇 |
2018年 | 15篇 |
2017年 | 15篇 |
2016年 | 40篇 |
2015年 | 61篇 |
2014年 | 67篇 |
2013年 | 64篇 |
2012年 | 115篇 |
2011年 | 97篇 |
2010年 | 62篇 |
2009年 | 59篇 |
2008年 | 79篇 |
2007年 | 73篇 |
2006年 | 46篇 |
2005年 | 81篇 |
2004年 | 68篇 |
2003年 | 58篇 |
2002年 | 59篇 |
2001年 | 17篇 |
2000年 | 5篇 |
1999年 | 14篇 |
1998年 | 18篇 |
1997年 | 7篇 |
1996年 | 5篇 |
1995年 | 9篇 |
1994年 | 3篇 |
1993年 | 5篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 4篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1968年 | 2篇 |
1965年 | 1篇 |
1962年 | 2篇 |
1936年 | 1篇 |
排序方式: 共有1238条查询结果,搜索用时 15 毫秒
981.
Phylogenetic taxonomy is applied for the systematization of a new Hesionidae , rather than the traditional Linnean system, with an apomorphy-based definition of the name and without reference to rank. It is argued that biological diversity is better represented without species concepts, but that it is useful to specify when a name refers to a smallest known clade which currently cannot be further subdivided; for this we apply the newly introduced concept LITU (Least-Inclusive Taxonomic Unit) for the new taxon. LITUs are made identifiable by being italicised with lower-case initial letter; all other taxon names are italicised with capital initial letter. The Hesionidae is accordingly named capricornia , new taxon, and was found in shallow water at One Tree Island, Capricorn Group, southernmost part of the Great Barrier Reef. capricornia is small (length < 2 mm), and exhibits a number of larval Hesionidae characters, but is characterized by large paired ventrally situated penes on segment 9 in adult males. A cladistic parsimony analysis based on morphological characters of capricornia and a selection of other Hesionidae indicates that it belongs within Gyptini Pleijel 1998, and is the sister group of Amphiduros Hartman (1959) . 相似文献
982.
Metagenomic sequencing has contributed important new knowledge about the microbes that live in a symbiotic relationship with humans. With modern sequencing technology it is possible to generate large numbers of sequencing reads from a metagenome but analysis of the data is challenging. Here we present the bioinformatics pipeline MEDUSA that facilitates analysis of metagenomic reads at the gene and taxonomic level. We also constructed a global human gut microbial gene catalogue by combining data from 4 studies spanning 3 continents. Using MEDUSA we mapped 782 gut metagenomes to the global gene catalogue and a catalogue of sequenced microbial species. Hereby we find that all studies share about half a million genes and that on average 300 000 genes are shared by half the studied subjects. The gene richness is higher in the European studies compared to Chinese and American and this is also reflected in the species richness. Even though it is possible to identify common species and a core set of genes, we find that there are large variations in abundance of species and genes. 相似文献
983.
Ole Kirk Fredrik Bj rkling Sven E. Godtfredsen Thomas Ostenfeld Larsen 《Biocatalysis and Biotransformation》1992,6(2):127-134
The fatty acid specificity of the B-lipase derived from Candida antarctica was investigated in the synthesis of esters of ethyl D-glucopyranoside. The specificity was almost identical with respect to straight-chain fatty acids with 10 to 18 carbon atoms. However, lower fatty acids such as hexanoic and octanoic acid and the unsaturated 9-cis-octadecenoic acid were found to be poor substrates of the enzyme. As a consequence of this selectivity, these fatty acids were accumulated in the unconverted fraction when ethyl D-glucopyranoside was esterified with an excess of a mixture of fatty acids. This accumulation can reduce the overall effectiveness of the process as the activity of the lipase was found to be reduced when exposed to high concentrations of short-chain fatty acids. Finally, using a simplified experimental set-up, the specificity of the C. antarctica B-lipase was compared to the specificity of lipases derived from C. rugosa, Mucor miehei, Humicola, and Pseudomonas. Apart from the C. rugosa lipase, which exhibited a very poor performance, all the enzymes showed a very similar specificity with respect to fatty acids longer than octanoic acid while only the C. antarctica B-lipase showed activity towards sort-chain fatty acids. 相似文献
984.
Baxter A Chambers M Edfeldt F Edman K Freeman A Johansson C King S Morley A Petersen J Rawlins P Spadola L Thong B Van de Poël H Williams N 《Bioorganic & medicinal chemistry letters》2011,21(2):777-780
The first known non-covalent inhibitors of rhinovirus 3C protease (3CP) have been identified through fragment based screening and hit identification activities. 相似文献
985.
Streptomyces coelicolor undergoes distinct morphological changes as it grows on solid media where spores differentiate into vegetative and aerial mycelium that is followed by the production of spores. Deletion of bldA, encoding the rare tRNA(Leu) UAA, blocks development at the stage of vegetative mycelium formation. From previous data it appears that tRNA(Leu) UAA accumulates relatively late during growth while two other tRNAs do not. Here, we studied the expression of 17 different tRNAs including bldA tRNA, and the RNA subunit of the tRNA processing endoribonuclease RNase P. Our results showed that all selected tRNAs and RNase P RNA increased with time during development. However, accumulation of bldA tRNA and another rare tRNA(Leu) isoacceptor started at an earlier stage compared with the other tRNAs. We also introduced the bldA tRNA anticodon (UAA) into other tRNAs and introduced these into a bldA deletion strain. In particular, one such mutant tRNA derived from the tRNA(Leu) CAA isoacceptor suppressed the bldA phenotype. Thus, the bldA tRNA scaffold is not critical for function as a regulator of S. coelicolor cell differentiation. Further substitution experiments, in which the 5'- and 3'-flanking regions of the suppressor tRNA were changed, indicated that these regions were important for the suppression. 相似文献
986.
This study explored if boldness could be used to predict social status. First, boldness was assessed by monitoring individual zebrafish behaviour in (1) an unfamiliar barren environment with no shelter (open field), (2) the same environment when a roof was introduced as a shelter, and (3) when the roof was removed and an unfamiliar object (Lego® brick) was introduced. Next, after a resting period of minimum one week, social status of the fish was determined in a dyadic contest and dominant/subordinate individuals were determined as the winner/loser of two consecutive contests. Multivariate data analyses showed that males were bolder than females and that the behaviours expressed by the fish during the boldness tests could be used to predict which fish would later become dominant and subordinate in the ensuing dyadic contest. We conclude that bold behaviour is positively correlated to dominance in zebrafish and that boldness is not solely a consequence of social dominance. 相似文献
987.
988.
Erik Chorell Emma Andersson Margery L. Evans Neha Jain Anna G?theson J?rgen ?den Matthew R. Chapman Fredrik Almqvist Pernilla Wittung-Stafshede 《PloS one》2015,10(10)
Amyloid formation is historically associated with cytotoxicity, but many organisms produce functional amyloid fibers (e.g., curli) as a normal part of cell biology. Two E. coli genes in the curli operon encode the chaperone-like proteins CsgC and CsgE that both can reduce in vitro amyloid formation by CsgA. CsgC was also found to arrest amyloid formation of the human amyloidogenic protein α-synuclein, which is involved in Parkinson’s disease. Here, we report that the inhibitory effects of CsgC arise due to transient interactions that promote the formation of spherical α-synuclein oligomers. We find that CsgE also modulates α-synuclein amyloid formation through transient contacts but, in contrast to CsgC, CsgE accelerates α-synuclein amyloid formation. Our results demonstrate the significance of transient protein interactions in amyloid regulation and emphasize that the same protein may inhibit one type of amyloid while accelerating another. 相似文献
989.
990.
Animal behavioral patterns can help us understand physiological and ecological constraints on animals and its influence on fitness. The surfacing patterns of aquatic air-breathing mammals constitute a behavioral pattern that has evolved as a trade-off between the need to replenish oxygen stores at the surface and the need to conduct other activities underwater. This study aims to better understand the surfacing pattern of a marine top predator, the minke whale (Balaenoptera acutorostrata), by investigating how their dive duration and surfacing pattern changes across their activity range. Activities were classified into resting, traveling, surface feeding and foraging at depth. For each activity, we classified dives into short and long dives and then estimated the temporal dependence between dive types. We found that minke whales modified their surfacing pattern in an activity-specific manner, both by changing the expression of their dives (i.e. density distribution) and the temporal dependence (transition probability) between dive types. As the depth of the prey layer increased between activities, the surfacing pattern of foraging whales became increasingly structured, going from a pattern dominated by long dives, when feeding at the surface, to a pattern where isolated long dives were followed by an increasing number of breaths (i.e. short dives), when the whale was foraging at depth. A similar shift in surfacing pattern occurred when prey handling time (inferred from surface corralling maneuvers) increased for surface feeding whales. The surfacing pattern also differed between feeding and non-feeding whales. Resting whales did not structure their surfacing pattern, while traveling whales did, possibly as a way to minimize cost of transport. Our results also suggest that minke whales might balance their oxygen level over multiple, rather than single, dive cycles. 相似文献