全文获取类型
收费全文 | 335篇 |
免费 | 19篇 |
出版年
2021年 | 3篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 2篇 |
2016年 | 3篇 |
2015年 | 6篇 |
2014年 | 11篇 |
2013年 | 13篇 |
2012年 | 21篇 |
2011年 | 11篇 |
2010年 | 14篇 |
2009年 | 10篇 |
2008年 | 22篇 |
2007年 | 20篇 |
2006年 | 14篇 |
2005年 | 15篇 |
2004年 | 16篇 |
2003年 | 24篇 |
2002年 | 37篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 5篇 |
1998年 | 7篇 |
1997年 | 9篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 5篇 |
1991年 | 7篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 6篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 6篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1980年 | 6篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1976年 | 3篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有354条查询结果,搜索用时 93 毫秒
101.
Molecular evidence from ascidians for the evolutionary origin of vertebrate cranial sensory placodes
Mazet F Shimeld SM 《Journal of experimental zoology. Part B. Molecular and developmental evolution》2005,304(4):340-346
Cranial sensory placodes are specialised areas of the head ectoderm of vertebrate embryos that contribute to the formation of the cranial sense organs and associated ganglia. Placodes are often considered a vertebrate innovation, and their evolution has been hypothesised as one key adaptation underlying the evolution of active predation by primitive vertebrates. Here, we review recent molecular evidence pertinent to understanding the evolutionary origin of placodes. The development of vertebrate placodes is regulated by numerous genes, including members of the Pax, Six, Eya, Fox, Phox, Neurogenin and Pou gene families. In the sea squirt Ciona intestinalis (a basal chordate and close relative of the vertebrates), orthologues of these genes are deployed in the development of the oral and atrial siphons, structures used for filter feeding by the sessile adult. Our interpretation of these findings is that vertebrate placodes and sea squirt siphon primordia have evolved from the same patches of specialised ectoderm present in the common ancestor of the chordates. 相似文献
102.
103.
In the digestive tract, the transit of ingested food induces a local contraction-relaxation reflex of which the smooth muscle cell (SMC) represents the functional unit. Although freshly isolated SMCs have been extensively used for in vitro studies, in specific cases cultured cells appear necessary. Because conventionally cultured SMCs lose their contractile properties, we have developed: (1) differentiated, contractile rabbit gastric SMCs (D-stim cells), cultured in a medium supplemented with insulin, and (2) proliferative, dedifferentiated rabbit gastric SMCs (P-stim cells), cultured in a medium supplemented with insulin, fetal serum, EGF and b-FGF. The proliferative index was 5±4% and 82±10%, respectively, for D-stim and P-stim cells. Expression of SM-myosin heavy chain was observed in 90% of D-stim cells, whereas it was progressively lost in P-stim cells. Carbachol (1–100 nM), glicentin (2 nM) and gastrin-17 (100 nM) induced contraction of D-stim cells cultured for 3 or 6 days, whereas they did not induce the contraction of P-stim cells; in contrast, gastrin-17 (10 nM) was able to stimulate DNA synthesis (1.86±0.09-fold increase) in P-stim cells. The coupling of muscarinic receptors to intracellular transduction pathways was evaluated in D-stim cells: at day 3, carbachol (100 nM) induced a twofold increase in the production of inositol tri-tetra-phosphates; in parallel, a phosphorylation of ERK MAP kinases occurred within 1 min of carbachol stimulation. In conclusion, cultured functional myocytes derived from mature tissue may be used for long-term studies concerning the events coupled either to proliferation or to motility regulation of differentiated SMCs due to the activation of G-protein-coupled receptors.This study was supported in part by grants from the AFM (Association Française contre les Myopathies). 相似文献
104.
Boyer J Bernardes-Genisson V Farines V Souchard JP Nepveu F 《Free radical research》2004,38(5):459-471
A series of 2-alkyl and 2-aryl substituted-3H-indol-3-one-1-oxides was prepared and evaluated for its radical trapping properties. Spin trapping and electron paramagnetic resonance experiments demonstrate the ability of these indolone-1-oxides to trap hetero- and carbon-centered radicals. The most stable spin adducts (lifetime of several hours) are obtained with 2-alkyl substituted nitrones, the 2-ethyl-5,6-dioxolo-3H-indolone-1-oxide, 5e and the 2-secbutyl-3H-indolone-1-oxide, 5f. These two nitrones are also sensitive to redox reactions in solution. Therefore this indolone-1-oxide series lacking a β-hydrogen atom gives rise to highly stable adducts with free radicals. 相似文献
105.
Systematic characterization of the taxoids in the needles of Taxus canadensis led to the discovery of seven taxanes along with three known congeners. Their structures were rigorously established by spectroscopic methods as 15-benzoyl-10-deacetyl-2-debenzoyl-10-dehydro-abeo-baccat in III; 15-benzoyl-2-debenzoyl-7, 9-dideacetyl-abeo-baccatin VI; N-acetyl-N-debenzoyltaxol; 7,9,13-trideacetylbaccatin VI; 10-deacetyl-10-glycolylbaccatin IV; 1 beta-hydroxy-10-deacetyl-10-glycolylbaccatin I; and 7-deacetyltaxuspine L. These taxanes, specific to the Canadian yew, were co-isolated with taxacustin, taxagifine and 2-deacetyl-7,10-diacetyl-5-deaminoacyl taxine A previously found in Taxus cuspidata, baccata, and yunnanensis, respectively. 相似文献
106.
107.
108.
109.
Host larvae of Drosophila melanogaster injected with the eicosanoid biosynthesis inhibitor, dexamethasone, prior to parasitization by the wasp Leptopilina boulardi, exhibited significantly reduced rates of melanotic encapsulation in comparison with control and saline-injected larvae. The results of this investigation suggest that prostaglandins and other eicosanoids are involved as cell-signaling molecules in the hemocytic encapsulation reaction of D. melanogaster larvae. 相似文献
110.
An integrated linkage-radiation hybrid map of the canine genome 总被引:12,自引:0,他引:12
Cathryn S. Mellersh Christophe Hitte Melissa Richman Francoise Vignaux Catherine Priat Sophie Jouquand Petra Werner Catherine André Susan DeRose Donald F. Patterson Elaine A. Ostrander Francis Galibert 《Mammalian genome》2000,11(2):120-130
Purebred dogs are a unique resource for dissecting the molecular basis of simple and complex genetic diseases and traits.
As a result of strong selection for physical and behavioral characteristics among the 300 established breeds, modern dogs
are characterized by high levels of interbreed variation, complemented by significant intrabreed homogeneity. A high-resolution
map of the canine genome is necessary to exploit the mapping power of this unusual resource. We describe here the integration
of an expanded canine radiation hybrid map, comprised of 600 markers, with the latest linkage map of 341 markers, to generate
a map of 724 markers—the densest map of the canine genome described to date. Through the inclusion of 217 markers on both
the linkage and RH maps, the 77 RH groups are reduced to 44 syntenic groups, thus providing comprehensive coverage of most
of the canine genome.
Received: 10 June 1999 / Accepted: 23 September 1999 相似文献