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41.
Viviane de Moura Linck Adriana Lourenço da Silva Micheli Figueiró Ângelo Luis Piato Ana Paula Herrmann Franciele Dupont Birck Elina Bastos Caramão Domingos Sávio Nunes Paulo Roberto H. Moreno Elaine Elisabetsky 《Phytomedicine》2009,16(4):303-307
Linalool is a monoterpene often found as a major component of essential oils obtained from aromatic plant species, many of which are used in traditional medical systems as hypno-sedatives. Psychopharmacological evaluations of linalool (i.p. and i.c.v.) revealed marked sedative and anticonvulsant central effects in various mouse models. Considering this profile and alleged effects of inhaled lavender essential oil, the purpose of this study was to examine the sedative effects of inhaled linalool in mice. Mice were placed in an inhalation chamber during 60 min, in an atmosphere saturated with 1% or 3% linalool.Immediately after inhalation, animals were evaluated regarding locomotion, barbiturate-induced sleeping time, body temperature and motor coordination (rota-rod test). The 1% and 3% linalool increased (p<0.01) pentobarbital sleeping time and reduced (p<0.01) body temperature. The 3% linalool decreased (p<0.01) locomotion. Motor coordination was not affected. Hence, linalool inhaled for 1 h seems to induce sedation without significant impairment in motor abilities, a side effect shared by most psycholeptic drugs. 相似文献
42.
Mrcio Jos Silveira Sidinei Magela Thomaz Roger Paulo Mormul Franciele Pereira Camacho 《International Review of Hydrobiology》2009,94(2):169-178
We tested the effects of desiccation and type of sediment (clay or sand) on the regeneration of fragments of the apical shoots of three species of Hydrocharitaceae: Egeria najas, E. densa and Hydrilla verticillata. The shoots were left to dry on clay or sand from zero to four days, and were then returned to aquaria containing water. To approximate natural conditions, the aquaria containing clay had higher turbidity and nutrient (P and N) concentrations than did the aquaria with sand. All species dried faster on sand substrate, which led to lower regeneration (in terms of dry weight, length, sprout and root formation) in this treatment. H. verticillata fragments elongated faster than the other species, but E. najas was the most successful species (in terms of increase in dry weight) in the sand treatment. Our results indicate that the exotic H. verticillata has a competitive advantage, at least in its early stages of regeneration, over the other two, native species, especially in more eutrophic and turbid habitats; whereas E. najas fragments have a competitive advantage in less‐turbid, oligotrophic and sand‐dominated sites. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
43.
Franciele Roberta Maldaner Francisco José Lima Aragão Flávia Barreto dos Santos Octavio Luiz Franco Monique da Rocha Queiroz Lima Renato de Oliveira Resende Raquel Medeiros Vasques Tatsuya Nagata 《Applied microbiology and biotechnology》2013,97(13):5721-5729
Dengue virus causes about 100 million cases of dengue disease per year in the world. Laboratory diagnosis is done mainly by serological techniques, which in many cases use crude virus extracts that may cause cross-reactions to other flaviviruses. These undesirable cross-reactions can be reduced or eliminated by using recombinant proteins based on restricted epitopes. Aiming to decrease flaviviral cross-reactions and non-specific interactions in dengue serological assays, a plant expression system was chosen for recombinant antigen production as a reliable and inexpensive dengue diagnostic tool. In the present report, the lettuce plastid transformation system was applied to achieve efficient and stable tetra-epitope peptide antigen production, and its reactivity was evaluated. For this purpose, one putative epitope at positions 34 to 57 of E protein within the junction site of domains I and II of dengue virus (DENV) 1 to 4 serotypes linked by glycine linkers was expressed in lettuce chloroplasts. The potential immunoreactivity for the four DENV serotypes was evaluated using sera from patients of positive and negative dengue cases. Results indicated an overall sensitivity of 71.7 % and specificity of 100 %. No cross-reactions with the sera of yellow fever-positive or healthy individuals vaccinated against yellow fever were observed. This novel approach may provide an alternative system for the large-scale production of dengue recombinant antigens useful for serodiagnosis. 相似文献
44.
Franciele Santos Maria Fernanda G. V. Pe?aflor Paul W. Paré Patrícia A. Sanches Aline C. Kamiya Mateus Tonelli Cristiane Nardi José Mauricio S. Bento 《PloS one》2014,9(11)
A number of soil-borne microorganisms, such as mycorrhizal fungi and rhizobacteria, establish mutualistic interactions with plants, which can indirectly affect other organisms. Knowledge of the plant-mediated effects of mutualistic microorganisms is limited to aboveground insects, whereas there is little understanding of what role beneficial soil bacteria may play in plant defense against root herbivory. Here, we establish that colonization by the beneficial rhizobacterium Azospirillum brasilense affects the host selection and performance of the insect Diabrotica speciosa. Root larvae preferentially orient toward the roots of non-inoculated plants versus inoculated roots and gain less weight when feeding on inoculated plants. As inoculation by A. brasilense induces higher emissions of (E)-β-caryophyllene compared with non-inoculated plants, it is plausible that the non-preference of D. speciosa for inoculated plants is related to this sesquiterpene, which is well known to mediate belowground insect-plant interactions. To the best of our knowledge, this is the first study showing that a beneficial rhizobacterium inoculant indirectly alters belowground plant-insect interactions. The role of A. brasilense as part of an integrative pest management (IPM) program for the protection of corn against the South American corn rootworm, D. speciosa, is considered. 相似文献
45.
Abigail Lopes Thais Ceresér Vilela Luciane Taschetto Franciele Vuolo Fabricia Petronilho Felipe Dal-Pizzol Emilio Luiz Streck Gustavo Costa Ferreira Patrícia Fernanda Schuck 《Molecular neurobiology》2014,50(3):1124-1130
Hereditary fructose intolerance is an autosomal recessive disorder characterized by the accumulation of fructose in tissues and biological fluids of patients. The disease results from a deficiency of aldolase B, responsible for metabolizing fructose in the liver, kidney, and small intestine. We investigated the effect of acute fructose administration on oxidative stress and neuroinflammatory parameters in the cerebral cortex of 30-day-old Wistar rats. Animals received subcutaneous injection of sodium chloride (0.9 %) (control group) or fructose solution (5 μmol/g) (fructose group). One hour later, the animals were euthanized and the cerebral cortex was isolated. Oxidative stress (levels of thiobarbituric acid-reactive substances (TBA-RS), carbonyl content, nitrate and nitrite levels, 2′,7′-dihydrodichlorofluorescein (DCFH) oxidation, glutathione (GSH) levels, as well as the activities of catalase (CAT) and superoxide dismutase (SOD)) and neuroinflammatory parameters (TNF-α, IL-1β, and IL-6 levels and myeloperoxidase (MPO) activity) were investigated. Acute fructose administration increased levels of TBA-RS and carbonyl content, indicating lipid peroxidation and protein damage. Furthermore, SOD activity increased, whereas CAT activity was decreased. The levels of GSH, nitrate, and nitrite and DCFH oxidation were not altered by acute fructose administration. Finally, cytokines IL-1β, IL-6, and TNF-α levels, as well as MPO activity, were not altered. Our present data indicate that fructose provokes oxidative stress in the cerebral cortex, which induces oxidation of lipids and proteins and changes of CAT and SOD activities. It seems therefore reasonable to propose that antioxidants may serve as an adjuvant therapy to diets or to other pharmacological agents used for these patients, to avoid oxidative damage to the brain. 相似文献
46.
Souza Leandro Cattelan Jesse Cristiano R. de Gomes Marcelo Gomes del Fabbro Lucian Goes André Tiago Rossito Donato Franciele Boeira Silvana Peterini 《Neurochemical research》2017,42(10):2982-2995
Neurochemical Research - There is a lack of information concerning the molecular events underlying the depressive-like effect of an intracerebroventricular injection of streptozotocin (ICV-STZ) in... 相似文献
47.
Anti‐Inflammatory and Anti‐Oxidant Effects of p‐Chloro‐phenyl‐selenoesterol on TNBS‐Induced Inflammatory Bowel Disease in Mice
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Micheli Stéfani Zarzecki Vandreza Cardoso Bortolotto Márcia Rósula Poetini Stífani Machado Araujo Mariane Trindade de Paula Silvane Souza Roman Cristiano Spiazzi Franciele Weber Santos Cibin Oscar Endrigo Dorneles Rodrigues Cristiano Ricardo Jesse Marina Prigol 《Journal of cellular biochemistry》2017,118(4):709-717
48.
Juliana Alves Josahkian Franciele Barbosa Trapp Maira Graeff Burin Kristiane Michelin-Tirelli Ana Paula Pereira Scholz de Magalhes Fernanda Medeiros Sebastio Fernanda Bender Jurema Ftima De Mari Ana Carolina Brusius-Facchin Sandra Leistner-Segal Diana Rojas Mlaga Roberto Giugliani 《Genetics and molecular biology》2021,44(1)
The mucopolysaccharidoses (MPS) are a group of lysosomal storage disorders caused by 11 enzyme deficiencies, classified into seven types. Data on the birth prevalence of each MPS type are available for only a few countries, and the totality of cases may be underestimated. To determine the epidemiological profile of MPS in each Brazilian region, we analyzed data collected between 1982 and 2019 by a national reference laboratory and identified 1,652 patients. Using data between 1994 and 2018, the birth prevalence (by 100,000 live births) for MPS was 1.57. MPS II was the most common type of MPS in Brazil, and its birth prevalence was 0.48 (0.94 considering only male births). Regarding the number of cases per region, MPS II was the most frequent in the North and Center-West (followed by MPS VI), and also in the Southeast (followed by MPS I); MPS I and MPS II were the most common types in the South; and MPS VI was the most common in the Northeast (followed by MPS II). The differences observed in the relative frequencies of MPS types across Brazilian regions are likely linked to founder effect, endogamy, and consanguinity, but other factors may be present and need further investigation. 相似文献
49.
Franciele Cabral Pinheiro Rodrigo Ligabue-Braun Ana Cecília Menezes de Siqueira Camila Matuella Carolina Fischinger Moura de Souza Fabíola Paoli Monteiro Fernando Kok Ida Vanessa Doederlein Schwartz Fernanda Sperb-Ludwig 《Genetics and molecular biology》2021,44(2)
Fructose-1,6-bisphosphatase (FBPase) deficiency is a rare inborn error of fructose metabolism caused by pathogenic variants in the FBP1 gene. As gluconeogenesis is affected, catabolic episodes can induce ketotic hypoglycemia in patients. FBP1 analysis is the most commonly used approach for the diagnosis of this disorder. Herein, a Brazilian patient is reported. The proband, a girl born to a consanguineous couple, presented with severe hypoglycemia crisis in the neonatal period. At the age 17 months, presented a new crisis accompanied by metabolic acidosis associated with a feverish episode. Genetic analysis was performed by next-generation sequencing (NGS), identifying the :c.611_614del variant in homozygosis in the FBP1 gene. In silico analysis and 3D modeling were performed, suggesting that this variant is associated with a loss of sites for substrate and Mg2+ binding and for posttranslational modifications of FBPase. The c.611_614del variant is located in a repetitive region of the FBP1 gene that appears to be a hotspot for mutational events. This frameshift creates a premature termination codon in the last coding exon which escapes the nonsense-mediated decay mechanism, according to in silico analysis. This variant results in an intrinsically disordered protein with loss of substrate recognition and post-translational modification sites. NM_000507.3相似文献
50.
Gabriela Merker Breyer Sílvia de Carli Natã Vieira Inácio Vânia Maria França Ribeiro Flavio Roberto Chaves da Silva Cíntia Daudt Franciele Maboni Siqueira 《Letters in applied microbiology》2022,75(1):10-16
Capybaras are rodent widely distributed in South America, which inhabit lakeside areas including ecological parks and urban sites. Due to anthropological interaction, monitoring zoonotic pathogens in wildlife is essential for One Health. We investigated faecal samples from capybaras living in an urban area in Rio Branco (Acre, Brazil) for the presence diarrhoeagenic E. coli. Virulence factors from shiga toxin-producing E. coli (STEC), enterohaemorrhagic E. coli (EHEC), and enteropathogenic E. coli (EPEC) were screened by PCR. We detected at least one virulence factor in 81% of the animals, being classified as STEC and EHEC pathotypes. The presence of zoonotic E. coli in capybaras is a warning due to the highly frequent anthropological interactions with wild animals in this area. Our findings highlight the importance of investigating wild animals as carriers of zoonotic E. coli, requiring further investigations into wildlife surveillance and epidemiological monitoring. 相似文献