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301.
Planktonic bacterial production in the tidal freshwater Hudson River is a major component of secondary productivity and is
uncoupled from planktonic primary productivity. There are several major sources of allochthonous dissolved organic carbon
(DOC) whose potential contribution to heterotrophic bacterial growth was examined with bioassays. Supply of DOC from the upper
Hudson drainage basin and a large tributary in the mid-Hudson together comprise 70 kT DOC/year, which is the bulk of the DOC
load to the tidal freshwater Hudson River. Two contrasting tidal wetlands contribute DOC to the main-stem river but were only
a few percent of the tributary load even during summer low-flow conditions. The quantity of DOC released from fine sediments
was intermediate to the other two loadings considered. Bacterial growth in bioassays receiving water from the sources varied,
but differences in thymidine incorporation between reference and DOC sources were small, usually less than 2 nmol/L/h. Similarity
in thymidine incorporation suggests that all sources of DOC were capable of supporting bacterial growth at approximately equal
rates. Seasonal shifts in carbon availability were clear in several cases, for example, greater growth on wetland-derived
DOC at times of peak plant productivity. Seasonal differences in tributary DOC bioavailability were not large despite the
well-known seasonality of tributary inputs. Activities of a suite of extracellular enzymes were used as a biologically based
characterization of DOC from the various sources. Shifts in allocation among enzymes were apparent, indicating that there
are biologically relevant differences in composition among the sources. Fluorescence characteristics and absorbance per unit
carbon also varied among sources, providing an independent confirmation of compositional differences among sources. The absence
of large differences in bacterial productivity among sources suggests that growth is supported by a wide range of DOC, and
the relative importance of the sources is probably related to the quantitative differences in inputs. Efforts to classify
carbon supplies to ecosystems must recognize that organism plasticity in carbon use and physical mixing processes will both
act to homogenize what might initially appear to be quite distinctive carbon inputs.
Received 15 April 1997; accepted 17 February 1998 相似文献
302.
Paolo Franchini Paolo Colangelo Emanuela Solano Ernesto Capanna Erik Verheyen Riccardo Castiglia 《Evolution; international journal of organic evolution》2010,64(7):2020-2032
The West European house mouse, Mus musculus domesticus, is a particularly suitable model to investigate the role of chromosomal rearrangements in reproductive isolation. In fact, it exhibits a broad range of chromosomal polymorphism due to Robertsonian (Rb) fusions leading to various types of contact zones between different chromosomal races. In the present study, we analyzed a parapatric contact in central Italy between the Cittaducale chromosomal race (CD: 2n= 22) and the surrounding populations with standard karyotype (2n= 40) to understand if Rb fusions play a causative role in speciation. One hundred forty‐seven mice from 17 localities were genotyped by means of 12 microsatellite loci. A telomeric and a pericentromeric locus situated on six chromosome arms (four Rbs and one telocentric) were selected to detect differences in the amount of gene flow for each locus in different chromosomal positions. The analyses performed on the two subsets of loci show differences in the level of gene flow, which is more restricted near the centromeres of Rb chromosomes. This effect is less pronounced in the homozygotes populations settled at the border of the hybrid zone. We discuss the possible cause of the differential porosity of gene flow in Rbs considering “hybrid dysfunctions” and “suppressed recombination” models. 相似文献
303.
304.
Ultrastructural studies were performed on the blood cells of Planorbarius corneus. Spreading and round cell forms were observed. The spreading form can be distinguished from the round form by its abundant cytoplasm, pseudopods and irregularly shaped nucleus. Electron dense granules in the mitochondrial matrix are found in the round form and are lacking in the spreading form. Moreover, cellular apoptosis is observed in the round form. The ultrastructural findings and the previously described characteristics support the idea that the two cell forms belong to two basic cell types. 相似文献
305.
Barr James Charles Broquet J. P. Cardamatis Aldo Castellani James Crichton-Browne G. Franchini Greenwood W. G. King Ewen J. MacLean Bernard Nocht G. H. F. Nuttall Edorado Perroncito Robert L. Pitfield Humphry Rolleston C. S. Sherrington David Thomson J. G. Thomson Malcolm Watson F. Parkes Weber 《BMJ (Clinical research ed.)》1929,1(3566):879-880
306.
EF-hand peptides have been shown to bind calcium and dimerize to form an intact protein domain [Shaw, G.S., Hodges, R.S. & Sykes, B.D. (1990). Science, 249, 280-283]. A synthetic 33-residue EF-hand peptide with the sequence of carp parvalbumin CD site demonstrated a seven-fold increase in the apparent calcium dissociation constant with a eight-fold decrease in peptide concentration when fit to a single-site calcium-binding model. This observation is consistent with EF-hand dimerization. This paper describes a method to determine the dimerization dissociation constant and the calcium dissociation constants for both the monomer and dimer forms of this EF-hand peptide using circular dichroism techniques. By monitoring the increase in negative molar ellipticity at 222 nm with increasing peptide concentration under calcium-saturating conditions the dimerization dissociation constant for the synthetic parvalbumin CD site was determined to be 55.68+/-10.76 microM. Using the dimerization constant, the calcium dissociation constants for both the monomer and dimer forms of this peptide were determined by monitoring the change in ellipticity of peptide solutions on addition of increasing amounts of calcium. A fit of this data to a mathematical model that takes into account dimerization results in calcium dissociation constants of 421.3+/-21.56 and 47.06+/-6.72 microM for the monomer and dimer forms, respectively. 相似文献
307.
Gregory A. Dekaban Laura Digilio Genoveffa Franchini 《Current opinion in genetics & development》1995,5(6)
The past five years have seen significant advances in understanding the origin and evolution of human T-cell leukemia/lymphotropic virus types I and II. The highlights include the identification of human T-cell leukemia/lymphotropic virus types I and II genotypic variants in remote human populations and the discovery of widely divergent simian T-cell leukemia virus in African and Asian non-human primates. 相似文献