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排序方式: 共有231条查询结果,搜索用时 31 毫秒
131.
Fragoso MC Kohek MB Martin RM Latronico AC Lucon AM Zerbini MC Longui CA Mendonca BB Domenice S 《Hormone research》2007,67(1):7-11
OBJECTIVE: Hormone-secreting adrenocortical tumors are frequently associated with endocrine syndromes. We describe a 30-year-old man who had abdominal pain, a nodule in the right breast and loss of libido. Abdominal magnetic resonance imaging revealed a very large tumor in the right adrenal gland. METHODS: Hormonal profile disclosed increased levels of estradiol and slightly low testosterone levels. The basal and stimulated LH levels were normal, whereas basal and stimulated FSH levels were totally suppressed. Cortisol and adrenal androgen levels were normal. The unusual finding of selective FSH suppression suggested secretion of inhibin B by the adrenocortical tumor. A very high level of serum inhibin B (405 pg/ml) was demonstrated by ELISA assay. Right adrenalectomy and nephrectomy were performed and the tumor was classified as a malignant tumor (Weiss score: 7.0) and unilateral mastectomy disclosed a lipoma. RESULTS: One week after surgery, a GnRH-stimulation test disclosed normal basal and stimulated FSH levels and low levels of inhibin B and estradiol. Immunohistochemical analysis with anti-B-inhibin antibody revealed intense staining in the adrenocortical tumor cells. One month after surgery, an abdominal magnetic resonance imaging revealed a local recurrence of the tumor and a second surgery was performed with partial resection of the tumor and the patient died 1 year after the first surgery. CONCLUSION: We herein report the first inhibin B and estradiol-secreting adrenocortical carcinoma. The unusual selective inhibition of FSH secretion should be considered a valuable hormonal finding for the diagnosis of inhibin B-secreting adrenocortical tumors. 相似文献
132.
Gilberto Vargas-Alarcón Javier Angeles-Martínez Teresa Villarreal-Molina Edith Alvarez-León Rosalinda Posadas-Sánchez Guillermo Cardoso-Salda?a Julian Ramírez-Bello Nonanzit Pérez-Hernández Juan Gabriel Juárez-Rojas José Manuel Rodríguez-Pérez José Manuel Fragoso Carlos Posadas-Romero 《PloS one》2015,10(1)
Aim
The role of interleukin 17A (IL-17A) in the inflammatory process has caused interest in the potential significance of IL-17A as a biomarker for coronary artery disease (CAD). The aim of the present study was to evaluate the role of IL-17A gene polymorphisms as susceptibility markers for CAD in the Mexican population.Methods
Four IL-17A gene polymorphisms (rs8193036, rs3819024, rs2275913 and rs8193037) were genotyped by 5’ exonuclease TaqMan assays in a group of 900 patients with premature CAD and 667 healthy controls (with negative calcium score by computed tomography), seeking associations with CAD and other metabolic and cardiovascular risk factors using logistic regression analyses.Results
No single IL-17A polymorphism was associated with premature CAD, however two haplotypes (CAGG and TAGA) were significantly associated with increased risk of premature CAD (OR = 1.35, 95% CI: 1.00–1.84, P = 0.018 and OR = 2.09, 95% CI: 1.16–3.76, P = 0.003, respectively). Moreover, rs3819024 was associated with increased levels of visceral abdominal fat (P = 0.002) and rs8193036 was significantly associated with risk of central obesity (P = 0.020), hypertriglyceridemia (P = 0.027), and metabolic syndrome (P = 0.027) in the premature CAD group, under dominant models adjusted by age, gender, BMI, smoking history, alcohol consumption, and treatment.Conclusion
The results suggest that IL-17A haplotypes are involved in the risk of developing premature CAD and some IL-17A polymorphisms are associated with cardiovascular risk factors in Mexican individuals with premature CAD. 相似文献133.
134.
Adalid-Peralta L Fleury A García-Ibarra TM Hernández M Parkhouse M Crispín JC Voltaire-Proaño J Cárdenas G Fragoso G Sciutto E 《The Journal of parasitology》2012,98(1):142-148
Human neurocysticercosis (NC) is caused by Taenia solium larvae lodged in the central nervous system. Most cases occur with no, or mild, neurological symptoms. However, in some patients, neuroinflammation is exacerbated, leading to severe forms of the disease. Considering the critical role of regulatory T cells (Tregs) in balancing inflammation in chronic diseases, their participation in restraining the inflammatory response in NC was explored in the present study. The frequency of Tregs and their relationship with the level of the proliferative response, the level of activated lymphocytes, and the cytokines expressed were determined in severe NC patients compared with those from healthy donors. Significantly increased peripheral Tregs (CD4(+)CD25(high) and CD4(+)CD25(high)FoxP3(+), CD4(+)CD25(high)CTLA4(+), and CD4(+)CD25(high) IL10(+)) and a significant decrease in activated (CD38(+) and CD69(+)) T cells were observed in 19 NC patients versus 10 healthy subjects. Significantly increased Tregs in NC are accompanied by a depressed specific, and non-specific, lymphocyte proliferative response, and they negatively correlate with activated CD4(+)CD69(+) lymphocytes. Treg frequencies were also determined in cerebral spinal fluid for 8 of the 19 NC patients. A positive significant correlation between peripheral and local Tregs was observed. Here, we report for the first time data that support the possible contribution of local and systemic Tregs in limiting neuroinflammation in NC. 相似文献
135.
Leon Hilda Fragoso Dalila Leon Daniel Candelaria Carlos Serviere Elisa González-González Jorge 《Hydrobiologia》1993,(1):197-205
Tidal pools in the Mexican Tropical Pacific coast have received relatively little attention in spite of their considerable richness in species and wide distribution in the region.This paper presents the first characterization of the algal flora of this region. It analyzes the number and composition of species of the tidal pools of six localities with regard to geographical distribution and its seasonal variations as well as tidal level. 97 species are reported, 25 Chlorophyta, 23 Phaeophyta, 34 Rhodophyta and 15 Cyanophyta.Of that total of species, 63% were found in one locality, 23.7% in two, 11.3% in three and 1 % in 4 or 5 localities. Not one species was common to all of the pools.The highest number of species was found on pools of the middle and low intertidal where the Chlorophyta, Rhodophyta and Phaeophyta were the most abundant algae. Cyanophyta was more common in the supralittoral and high intertidal pools. 相似文献
136.
137.
Valdinei S. Martins Raildo M. de Jesus Erik G. P. da Silva Wallace Duarte Fragoso Sérgio L. C. Ferreira 《Biological trace element research》2011,143(1):478-488
The obi (Cola acuminate) is a native fruit from Africa, which has been mainly used in the production of soft drinks and also in rituals of African
religions. In this paper, the mineral composition of obi collected in seven different cities from Bahia State, Brazil was
determined and evaluated using multivariate analysis. The samples were digested using nitric acid and hydrogen peroxide and
were analyzed using inductively coupled plasma optical emission spectrometry. The accuracy of the method was confirmed by
analysis of a certified reference material of apple leaves, furnished by National Institute of Standard and Technology. The
study involved 46 samples, being 18 of the red specie and 28 for the white specie. The results expressed as milligrams of
element per 100 g−1 of sample demonstrated that the concentration ranges varied of 21.28–548.77 for potassium, 15.73–129.85 for phosphorous,
27.95–286.92 for calcium, 7.67–134.45 for magnesium, 0.05–1.41 for manganese, 0.21–0.94 for iron, 0.11–0.39 for copper, 0.27–1.35
for zinc, and 0.025–0.517 for strontium. The principal component analysis and hierarchical cluster analysis evidenced that
the mineral composition of the red specie is different of the white specie. The red obi has mineral content higher than white
obi. 相似文献
138.
Y Cheng X Ren A SP Gowda Y Shan L Zhang Y-S Yuan R Patel H Wu K Huber-Keener J W Yang D Liu T E Spratt J-M Yang 《Cell death & disease》2013,4(7):e731
Sirtuin 3 (Sirt3), a major mitochondrial NAD+-dependent deacetylase, targets various mitochondrial proteins for lysine deacetylation and regulates important cellular functions such as energy metabolism, aging, and stress response. In this study, we identified the human 8-oxoguanine-DNA glycosylase 1 (OGG1), a DNA repair enzyme that excises 7,8-dihydro-8-oxoguanine (8-oxoG) from damaged genome, as a new target protein for Sirt3. We found that Sirt3 physically associated with OGG1 and deacetylated this DNA glycosylase and that deacetylation by Sirt3 prevented the degradation of the OGG1 protein and controlled its incision activity. We further showed that regulation of the acetylation and turnover of OGG1 by Sirt3 played a critical role in repairing mitochondrial DNA (mtDNA) damage, protecting mitochondrial integrity, and preventing apoptotic cell death under oxidative stress. We observed that following ionizing radiation, human tumor cells with silencing of Sirt3 expression exhibited deteriorated oxidative damage of mtDNA, as measured by the accumulation of 8-oxoG and 4977 common deletion, and showed more severe mitochondrial dysfunction and underwent greater apoptosis in comparison with the cells without silencing of Sirt3 expression. The results reported here not only reveal a new function and mechanism for Sirt3 in defending the mitochondrial genome against oxidative damage and protecting from the genotoxic stress-induced apoptotic cell death but also provide evidence supporting a new mtDNA repair pathway. 相似文献
139.
Christopher Heffelfinger Christopher A Fragoso Maria A Moreno John D Overton John P Mottinger Hongyu Zhao Joe Tohme Stephen L Dellaporta 《BMC genomics》2014,15(1)
Background
Many areas critical to agricultural production and research, such as the breeding and trait mapping in plants and livestock, require robust and scalable genotyping platforms. Genotyping-by-sequencing (GBS) is a one such method highly suited to non-human organisms. In the GBS protocol, genomic DNA is fractionated via restriction digest, then reduced representation is achieved through size selection. Since many restriction sites are conserved across a species, the sequenced portion of the genome is highly consistent within a population. This makes the GBS protocol highly suited for experiments that require surveying large numbers of markers within a population, such as those involving genetic mapping, breeding, and population genomics. We have modified the GBS technology in a number of ways. Custom, enzyme specific adaptors have been replaced with standard Illumina adaptors compatible with blunt-end restriction enzymes. Multiplexing is achieved through a dual barcoding system, and bead-based library preparation protocols allows for in-solution size selection and eliminates the need for columns and gels.Results
A panel of eight restriction enzymes was selected for testing on B73 maize and Nipponbare rice genomic DNA. Quality of the data was demonstrated by identifying that the vast majority of reads from each enzyme aligned to restriction sites predicted in silico. The link between enzyme parameters and experimental outcome was demonstrated by showing that the sequenced portion of the genome was adaptable by selecting enzymes based on motif length, complexity, and methylation sensitivity. The utility of the new GBS protocol was demonstrated by correctly mapping several in a maize F2 population resulting from a B73 × Country Gentleman test cross.Conclusions
This technology is readily adaptable to different genomes, highly amenable to multiplexing and compatible with over forty commercially available restriction enzymes. These advancements represent a major improvement in genotyping technology by providing a highly flexible and scalable GBS that is readily implemented for studies on genome-wide variation.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-979) contains supplementary material, which is available to authorized users. 相似文献140.
Nick J Willett Tanushree Thote Angela SP Lin Shamus Moran Yazdan Raji Sanjay Sridaran Hazel Y Stevens Robert E Guldberg 《Arthritis research & therapy》2014,16(1):R47