Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping-based chromosomal homologies

Comparative chromosome painting with individual human chromosome-specific libraries (CSLs) on cattle metaphase chromosomes delineated 46 homologous chromosomal segments between the two species. Continuous arrangement of these segments on individual cattle chromosomes demonstrates a nearly complete coverage of the bovine karyotype and shows physical boundaries of bovine chromosomal segments homologous to individual human chromosomes. Alignment of the available comparative gene mapping data with the homologous segments strongly supports the detected gross homologies between the karyotypes of the two species. In addition to cattle, four human CSLs were hybridized to sheep metaphase chromosomes also, to further verify the known karyotype homology within the Bovidae. Besides its application to karyotype evolution research, the comparative knowledge provides for rapid expansion of the much needed Type I locus-based bovine gene map. Received: 9 September 1995 / Accepted: 4 December 1995     

A comparative study on the antioxidant effects of hesperidin and ellagic acid against skeletal muscle ischemia/reperfusion injury

Abstract

The antioxidant effects of ellagic acid (EA) and hesperidin (HES) against skeletal muscle ischemia/reperfusion injury (I/R) were performed. Hindlimb ischemia has been induced by tourniquet occlusion for 2?h on left hindlimb. At the end of ischemia, the tourniquate has been removed and initiated reperfusion for 2?h. EA (100?mg/kg) has been applied orally before ischemia/reperfusion in the EA?+?I/R group. HES (100?mg/kg) has been given orally in the HES?+?I/R group. The left gastrocnemius muscle has been harvested and stored immediately at??80?°C until assessed for the levels of MDA and antioxidant enzymes activities. MDA level has statistically increased in I/R group (p?<?0.05) compared to other groups. The muscle tissue antioxidant enzymes activities were lower than the other groups in the I/R group (p?<?0.05). EA and HES treatments significantly reversed the damage level in I/R, also activity of tissue SOD increased in the EA?+?I/R and HES?+?I/R groups.     

Über die Untersuchungen von A. H. Blaauw,betreffend die Beziehung zwischen Lichtintensität und Beleuchtungsdauer bei der phototropischen Krümmung von Keimlingen vonAvena sativa

Ohne ZusammenfassungProceedings of the Meetings of Saturday September 26, 1908.Die vorliegende Übersetzung, zu der Herr Prof. Went mich gütigst autorisierte, rechtfertigt sich durch die in theoretischer, ganz besonders aber in methodologischer Hinsicht überaus wichtigen Ergebnisse Blaauws. Die Untersuchung bestätigt zunächst ein Gesetz, das der Übersetzer für die Abhängigkeit der Präsentationszeit von der Lichtintensität festgestellt hat (die diesbezügliche Abhandlung war Herrn Prof. Went zur Zeit, als er dieses Referat schrieb, noch nicht zu Gesicht gekommen), verfolgt aber diese Gesetzmäßigkeit innerhalb wesentlich weiterer Grenzen. Die Feststellung ganz besonders, daß bei entsprechend intensiver Beleuchtung die Präsentationszeit bis auf 1/1000 Sekunde sinkt, zusammengehalten mit den Tatsachen der feinen Unterschiedsempfind-lichkeit der Pflanzen (Wiesner) und der außerordentlich kurzen Perzeptions-zeit (Fitting), muß die reizphysiologische Methodik reformieren und sie zu ebenso exaktem Arbeiten anspornen, wie es bei psychophysischen Experimenten längst der Fall ist.     

A bacterial clone carrying sequences coding for elongation factor EF-1 alpha from Artemia

A bacterial cDNA clone was identified carrying one third of the nucleotides coding for elongation factor EF-1 alpha from the brine shrimp Artemia. The sequence of codons corresponds with the known sequence of amino acids of EF-1 alpha in the region involved.     

A new semi-automated instrument to improve the fine needle aspiration procedure during breast lesion cell sampling

A large and increasing number of women in the western world will at some point during their life be investigated morphologically for some type of breast lesion. Fine Needle Aspiration (FNA) is one morphological method which is considered to be the fastest, cheapest and the most patient-friendly approach. However, the frequency of conclusive samples using this method varies and is often too low, especially when performed by unexperienced operators. In this study we have developed and tested a new semi-automated instrument (“CytoTest”) designed for FNA which is intended to improve the efficacy of the technique by increasing the percentage of conclusive samples. A total of 443 consecutive aspiration procedures on palpable breast lesions were performed to compare this new “CytoTest” equipment with the standard protocol using the same type of needles. We conclude that by increasing the extent and frequency of the reciprocatory motions used by an experienced sampling operator as well as enhancing the ejection pressure, the cellular yield can be increased almost three folded compared to the standard protocol. For cases with high amounts of non-diagnostic material (such as blood or cystic fluid) which were discarded, up to four times more sample could be obtained. Furthermore, the frequency of sparse samples under 1 mg was halved with use of the “CytoTest”.