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排序方式: 共有143条查询结果,搜索用时 185 毫秒
81.
Daryl R. Trumbo Patricia E. Salerno Kenneth A. Logan Mathew W. Alldredge Roderick B. Gagne Christopher P. Kozakiewicz Simona Kraberger Nicholas M. Fountain‐Jones Meggan E. Craft Scott Carver Holly B. Ernest Kevin R. Crooks Sue VandeWoude W. Chris Funk 《Molecular ecology》2019,28(22):4926-4940
Apex predators are important indicators of intact natural ecosystems. They are also sensitive to urbanization because they require broad home ranges and extensive contiguous habitat to support their prey base. Pumas (Puma concolor) can persist near human developed areas, but urbanization may be detrimental to their movement ecology, population structure, and genetic diversity. To investigate potential effects of urbanization in population connectivity of pumas, we performed a landscape genomics study of 130 pumas on the rural Western Slope and more urbanized Front Range of Colorado, USA. Over 12,000 single nucleotide polymorphisms (SNPs) were genotyped using double‐digest, restriction site‐associated DNA sequencing (ddRADseq). We investigated patterns of gene flow and genetic diversity, and tested for correlations between key landscape variables and genetic distance to assess the effects of urbanization and other landscape factors on gene flow. Levels of genetic diversity were similar for the Western Slope and Front Range, but effective population sizes were smaller, genetic distances were higher, and there was more admixture in the more urbanized Front Range. Forest cover was strongly positively associated with puma gene flow on the Western Slope, while impervious surfaces restricted gene flow and more open, natural habitats enhanced gene flow on the Front Range. Landscape genomic analyses revealed differences in puma movement and gene flow patterns in rural versus urban settings. Our results highlight the utility of dense, genome‐scale markers to document subtle impacts of urbanization on a wide‐ranging carnivore living near a large urban center. 相似文献
82.
Nicholas M. Fountain‐Jones Craig Packer Maude Jacquot F. Guillaume Blanchet Karen Terio Meggan E. Craft 《Ecology letters》2019,22(6):904-913
Pathogens are embedded in a complex network of microparasites that can collectively or individually alter disease dynamics and outcomes. Endemic pathogens that infect an individual in the first years of life, for example, can either facilitate or compete with subsequent pathogens thereby exacerbating or ameliorating morbidity and mortality. Pathogen associations are ubiquitous but poorly understood, particularly in wild populations. We report here on 10 years of serological and molecular data in African lions, leveraging comprehensive demographic and behavioural data to test if endemic pathogens shape subsequent infection by epidemic pathogens. We combine network and community ecology approaches to assess broad network structure and characterise associations between pathogens across spatial and temporal scales. We found significant non‐random structure in the lion‐pathogen co‐occurrence network and identified both positive and negative associations between endemic and epidemic pathogens. Our results provide novel insights on the complex associations underlying pathogen co‐occurrence networks. 相似文献
83.
In some species, populations with few founding individuals can be resilient to extreme inbreeding. Inbreeding seems to be the norm in the common bed bug, Cimex lectularius, a flightless insect that, nevertheless, can reach large deme sizes and persist successfully. However, bed bugs can also be dispersed passively by humans, exposing inbred populations to gene flow from genetically distant populations. The introduction of genetic variation through this outbreeding could lead to increased fitness (heterosis) or be costly by causing a loss of local adaptation or exposing genetic incompatibility between populations (outbreeding depression). Here, we addressed how inbreeding within demes and outbreeding between distant populations impact fitness over two generations in this re‐emerging public health pest. We compared fitness traits of families that were inbred (mimicking reproduction following a founder event) or outbred (mimicking reproduction following a gene flow event). We found that outbreeding led to increased starvation resistance compared to inbred families, but this benefit was lost after two generations of outbreeding. No other fitness benefits of outbreeding were observed in either generation, including no differences in fecundity between the two treatments. Resilience to inbreeding is likely to result from the history of small founder events in the bed bug. Outbreeding benefits may only be detectable under stress and when heterozygosity is maximized without disruption of coadaptation. We discuss the consequences of these results both in terms of inbreeding and outbreeding in populations with genetic and spatial structuring, as well as for the recent resurgence of bed bug populations. 相似文献
84.
Primary human skin fibroblasts are an accessible source of phenotypically and karyotypically normal human cells, but are difficult to transfect with exogenous DNA. Here we demonstrate that both transient expression and stable transformation can be carried out by the method of electroporation. Highly efficient transient chloramphenicol acetyltransferase expression was shown after transfection with plasmid pRSVCAT. Stable transformation of human skin fibroblasts to G418 resistance was obtained after electroporation with neo-containing plasmids at an efficiency of approximately 1.4 x 10(-5)/micrograms DNA. The ability to easily transfect these cells with exogenous DNA may have important applications in the study of human genetic diseases and cancer. 相似文献
85.
86.
R. W. Hofmann B. D. Campbell D. W. Fountain B. R. Jordan D. H. Greer D. Y. Hunt & C. L. Hunt 《Plant, cell & environment》2001,24(9):917-927
White clover (Trifolium repens L.) is experiencing increased levels of ultraviolet‐B (UV‐B) radiation in temperate pastures due to the depletion of the stratospheric ozone layer. Based on 17 morphological, morphogenetic and physiological attributes, this study analysed the consequences of enhanced UV‐B on 26 white clover populations using principal components analysis (PCA). After 18 d of exposure to 13·3 kJ m ? 2 d ? 1 UV‐B in controlled environments, UV‐B significantly decreased above‐ground and below‐ground plant growth attributes, epidermal cell surface area and maximum quantum efficiency of photosystem II photochemistry (Fv/Fm). Aspects of cell division and cell expansion both were negatively affected by UV‐B. Stomatal density, specific leaf mass, root‐to‐shoot ratio and levels of UV‐B‐absorbing compounds increased in response to UV‐B. In the multivariate analysis, the main dimension of UV‐B sensitivity was characterized by changes in plant growth attributes. Alterations in partitioning within and between plant organs constituted a secondary tier of UV‐B responsiveness. Plant characteristics related to UV‐B tolerance included lower growth rate, smaller epidermal cell surface area and higher UV‐B‐induced levels of UV‐B‐absorbing compounds. The results suggest overall UV‐B tolerance for slower‐growing populations from less productive habitats with higher natural UV‐B irradiance. 相似文献
87.
88.
89.
Experimentally increased snow accumulation alters soil moisture and animal community structure in a polar desert 总被引:1,自引:0,他引:1
Edward Ayres Johnson N. Nkem Diana H. Wall Byron J. Adams J. E. Barrett Breana L. Simmons Ross A. Virginia Andrew G. Fountain 《Polar Biology》2010,33(7):897-907
Snow accumulation can influence soil properties in arctic and alpine tundra, boreal and temperate forests, and temperate grasslands.
However, snow may be even more influential in arid ecosystems, which by definition are water limited, such as the hyper-arid
polar desert of the McMurdo Dry Valleys, Antarctica. Moreover, snow accumulation may be altered by climate change in the future.
In order to investigate the impact of changes in snow accumulation on soils in the McMurdo Dry Valleys we experimentally manipulated
the quantity of snow at two locations and monitored soil properties over 5 years in relation to a snow depth gradient created
by snow fences. We predicted that increased snow depth would be associated with increased soil moisture and a shift in soil
animal community structure. While we did not observe changes in soil biochemistry or community structure along the snow depth
gradient at either site, increased snow accumulation caused by the snow fence altered soil properties across the entire length
of the transects at one site (Fryxell), which collected substantially more snow than the other site. At Fryxell, the presence
of the snow fence increased gravimetric soil moisture from 1 to 5–9%. This was associated with a decline in abundance of the
dominant animal, Scottnema lindsayae, a nematode typically found in dry soil, and an increase in Eudorylaimus sp. a nematode associated with moist soil. We also observed changes in soil pH, salinity, and concentrations of inorganic
nitrogen and chlorophyll a over the course of the experiment, but it was difficult to determine if these were caused by snow accumulation or simply
represented temporal variation related to other factors. 相似文献
90.
Young MT Fisher JA Fountain SJ Ford RC North RA Khakh BS 《The Journal of biological chemistry》2008,283(38):26241-26251
P2X receptors are ATP-gated nonselective cation channels with important physiological roles. However, their structures are poorly understood. Here, we analyzed the architecture of P2X receptors using fluorescence resonance energy transfer (FRET) microscopy and direct structure determination using electron microscopy. FRET efficiency measurements indicated that the distance between the C-terminal tails of P2X(4) receptors was 5.6 nm. Single particle analysis of purified P2X(4) receptors was used to determine the three-dimensional structure at a resolution of 21A(;) the orientation of the particle with respect to the membrane was assigned by labeling the intracellular C termini with 1.8-nm gold particles and the carbohydrate-rich ectodomain with lectin. We found that human P2X(4) is a globular torpedo-like molecule with an approximate volume of 270 nm(3) and a compact propeller-shaped ectodomain. In this structure, the distance between the centers of the gold particles was 6.1 nm, which closely matches FRET data. Thus, our data provide the first views of the architecture, shape, and size of single P2X receptors, furthering our understanding of this important family of ligand-gated ion channels. 相似文献