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41.
Isolation and partial characterization of protein E, a major protein found in certain Escherichia coli K-12 mutant strains: relationship to other outer membrane proteins.
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Escherichia coli outer membrane protein E was purified, and its amino acid composition and N-terminal amino acid were determined. The purified protein was shown to be immunologically and electrophoretically identical to proteins Ic (U. Henning, W. Schmidmayr, and I. Hindennach, Mol. Gen. Genet. 154:293-298, 1977) and e (W. van Alphen, N. van Selm, and B. Lugtenberg, Mol. Gen. Genet. 159:75-83, 1978). Proteins E, e, and Ic were also immunologically related to E. coli outer membrane protein Ia. Lugtenberg and co-workers (B. Lugtenberg, R. van Boxtel, C. Verhoef, and W. van Alphen, FEBS Lett. 96:99-105, 1978) have shown that electrophoretically identical peptides were generated by cyanogen bromide treatment of proteins E, e, and Ic. 相似文献
42.
Escherichia coli K-12 tolF mutants: alterations in protein composition of the outer membrane. 总被引:22,自引:7,他引:15
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Outer membrane materials prepared from three independently isolated spontaneous Escherichia coli tolF mutants contained no detectable protein Ia. The loss of this protein was nearly completely compensated for by an increase in other major outer membrane proteins, Ib and II. Thus, the major outer membrane proteins accounted for 40% of the total cell envelope protein in both tol+ and tolF strains. No changes were found in the levels of inner membrane proteins prepared from tolF strains when compared with similar preparations from the tol+ strain. Phage-resistant mutants were selected starting with a tolF strain by using either phage TuIb or phage PA2. These phage-resistant tolF strains contained neither protein Ia nor protein Ib. The mutation leading to the loss of protein Ib in these strains is independent of the tolF mutation and is located near malP on the E. coli genetic map. 相似文献
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Concomitant Synthesis of Bacteriocin and Bacteriocin Inactivator from Serratia marcescens 总被引:5,自引:1,他引:4
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We have found that Serratia marcescens strain P & S is bacteriocinogenic. However, the phenotypic expression of bacteriocin activity depends upon the temperature at which the cells are grown. When the organism is grown at 30 to 37 C, no bacteriocin activity can be demonstrated, whereas when it is grown at 39 C bacteriocin activity is readily observed. It appears that the P & S strain concomitantly synthesizes a bacteriocin and a substance which not only can inactivate the bacteriocin but also has a high activation energy for inactivation. This inactivator readily loses its activity when heated at 39 C for 1 hr. Two mutants were isolated from the P & S strain which can produce active bacteriocin when grown at temperatures from 30 to 39 C. It is significant that these mutants have considerably less bacteriocin inactivator. The data suggest that the inactivator is an extracellular protease. The ability of one of these mutants, JF58-12, to produce active bacteriocin at temperatures between 30 and 39 C is a stable property, whereas in the other mutant, JF48W, this property is unstable. JF48W was selected from the P & S strain in two steps: first a streptomycin-resistant variant (strain A-10) was isolated and from this mutant a strain (JF48W) was isolated which not only synthesized little of the inactivator but also did not synthesize the red pigmnet prodigiosin. This latter pleiotropic mutant appears to revert in one step to a phenotype similar to the P & S strain, since it is streptomycin-sensitive and produces prodigiosin and normal amounts of inactivator and the demonstration of bacteriocin activity is temperature-dependent. 相似文献
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Jonathan Foulds 《BMJ (Clinical research ed.)》2000,320(7232):454
47.
New major outer membrane proteins found in an Escherichia coli tolF mutant resistant to bacteriophage TuIb. 总被引:27,自引:18,他引:9
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Cell envelopes prepared from an Escherichia coli tolF strain selected as resistant to phage TuIb contained a new major outer membrane protein related to outer membrane proteins Ia and Ib. The strain that produces this protein is a tolF par double mutant but contains an additional mutation leading to the production of the new major outer membrane protein. Antibiotic sensitivity lost as a result of the tolF mutation is regained in strains that contain the new major outer membrane protein. This indicates that this protein functions to restore the selective permeability of the outer membrane to low-molecular-weight hydrophilic molecules. 相似文献
48.
IL-10 is required for optimal CD8 T cell memory following Listeria monocytogenes infection 总被引:1,自引:0,他引:1
IL-10 is an important immunoregulatory cytokine that plays a central role in maintaining a balance between protective immunity against infection and limiting proinflammatory responses to self or cross-reactive Ags. We examined the full effects of IL-10 deficiency on the establishment and quality of T cell memory using murine listeriosis as a model system. IL-10(-/-) mice had reduced bacterial loads and a shorter duration of primary infection than did wild-type mice. However, the number of Ag-specific T cells in secondary lymphoid and nonlymphoid organs was diminished in IL-10(-/-) mice, compared with wild-type mice, at the peak of the effector response. Moreover, the frequency and protective capacity of memory T cells also were reduced in IL-10(-/-) mice when assessed up to 100 days postinfection. Remarkably, this effect was more pronounced for CD8 T cells than CD4 T cells. To address whether differences in the number of bacteria and duration of primary infection could explain these findings, both strains of mice were treated with ampicillin 24 hours after primary infection. Despite there being more comparable bacterial loads during primary infection, IL-10(-/-) mice still generated fewer memory CD8 T cells and were less protected against secondary infection than were wild-type mice. Finally, the adoptive transfer of purified CD8 T cells from previously infected wild-type mice into naive recipients conferred better protection than the transfer of CD8 T cells from immune IL-10(-/-) mice. Overall, these data show that IL-10 plays an unexpected role in promoting and/or sustaining CD8 T cell memory following Listeria monocytogenes infection. 相似文献
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