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排序方式: 共有218条查询结果,搜索用时 15 毫秒
211.
Rogier Bodewes Siamak Zohari Jesper S. Krog Matthew D. Hall Timm C. Harder Theo M. Bestebroer Marco W. G. van de Bildt Monique I. Spronken Lars E. Larsen Ursula Siebert Peter Wohlsein Christina Puff Frauke Seehusen Wolfgang Baumg?rtner Tero H?rk?nen Saskia L. Smits Sander Herfst Albert D. M. E. Osterhaus Ron A. M. Fouchier Marion P. Koopmans Thijs Kuiken 《Journal of virology》2016,90(9):4269-4277
212.
Bart L. Haagmans Judith M. A. van den Brand Lisette B. Provacia V. Stalin Raj Koert J. Stittelaar Sarah Getu Leon de Waal Theo M. Bestebroer Geert van Amerongen Georges M. G. M. Verjans Ron A. M. Fouchier Saskia L. Smits Thijs Kuiken Albert D. M. E. Osterhaus 《Journal of virology》2015,89(11):6131-6135
The ability of Middle East respiratory syndrome coronavirus (MERS-CoV) to infect small animal species may be restricted given the fact that mice, ferrets, and hamsters were shown to resist MERS-CoV infection. We inoculated rabbits with MERS-CoV. Although virus was detected in the lungs, neither significant histopathological changes nor clinical symptoms were observed. Infectious virus, however, was excreted from the upper respiratory tract, indicating a potential route of MERS-CoV transmission in some animal species. 相似文献
213.
The replicative helicase MCM recruits cohesin acetyltransferase ESCO2 to mediate centromeric sister chromatid cohesion
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Miroslav P Ivanov Rene Ladurner Ina Poser Rebecca Beveridge Evelyn Rampler Otto Hudecz Maria Novatchkova Jean‐Karim Hériché Gordana Wutz Petra van der Lelij Emanuel Kreidl James RA Hutchins Heinz Axelsson‐Ekker Jan Ellenberg Anthony A Hyman Karl Mechtler Jan‐Michael Peters 《The EMBO journal》2018,37(15)
Chromosome segregation depends on sister chromatid cohesion which is established by cohesin during DNA replication. Cohesive cohesin complexes become acetylated to prevent their precocious release by WAPL before cells have reached mitosis. To obtain insight into how DNA replication, cohesion establishment and cohesin acetylation are coordinated, we analysed the interaction partners of 55 human proteins implicated in these processes by mass spectrometry. This proteomic screen revealed that on chromatin the cohesin acetyltransferase ESCO2 associates with the MCM2‐7 subcomplex of the replicative Cdc45‐MCM‐GINS helicase. The analysis of ESCO2 mutants defective in MCM binding indicates that these interactions are required for proper recruitment of ESCO2 to chromatin, cohesin acetylation during DNA replication, and centromeric cohesion. We propose that MCM binding enables ESCO2 to travel with replisomes to acetylate cohesive cohesin complexes in the vicinity of replication forks so that these complexes can be protected from precocious release by WAPL. Our results also indicate that ESCO1 and ESCO2 have distinct functions in maintaining cohesion between chromosome arms and centromeres, respectively. 相似文献
214.
215.
The destruction of periodontal tissues during periodontitis is the result of the immune-inflammatory reactions to the bacteria of dental biofilm. Probiotics may reduce dysbiosis by the modification of the dental microbiome, which can influence the immune-inflammatory mechanisms. The aim of this study was to estimate the clinical and microbiological parameters, before and after 30 days of application of the dietary supplement containing Lactobacillus salivarius SGL03 or placebo. The study was conducted in 51 patients with stage I or II periodontitis during the maintenance phase of treatment. The clinical parameters and the number of colony forming units (CFU) of bacteria in supragingival plaque were assessed before and after 30 days of the oral once daily administration of the dietary supplement in the form of suspension containing L. salivarius SGL03 or placebo. There were no changes in the PI scores between and within the groups. The value of BOP decreased in both groups. In the study group the significant reduction of the mean pocket depth was revealed (from 2.5 to 2.42, p = 0,027) but without the difference between the groups. There were no significant changes in the number of bacteria within the groups. In the control, but not the study group, positive correlations were observed between the clinical parameters (variables) and the number of bacteria. The use of the dietary supplement containing L. salivarius SGL03 may reduce pocket depth despite the lack of changes in other clinical parameters and the number of bacteria in supragingival plaque.Key words: probiotics, periodontal treatment, Lactobacillus salivarius 相似文献
216.
In vitro, poorly iodinated thyroglobulin (Tg) is hydrolysed at the same rate whether it is enclosed in thyroid or in liver lysosomes, while fully iodinated Tg is degraded faster inside liver lysosomes. After in vivo TSH administration, thyroid lysosomes hydrolyse fully iodinated Tg as fast as do liver lysosomes. 相似文献
217.
V. Stalin Raj Saskia L. Smits Lisette B. Provacia Judith M. A. van den Brand Lidewij Wiersma Werner J. D. Ouwendijk Theo M. Bestebroer Monique I. Spronken Geert van Amerongen Peter J. M. Rottier Ron A. M. Fouchier Berend Jan Bosch Albert D.M.E. Osterhaus Bart L. Haagmans 《Journal of virology》2014,88(3):1834-1838
Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in cells of different species using dipeptidyl peptidase 4 (DPP4) as a functional receptor. Here we show the resistance of ferrets to MERS-CoV infection and inability of ferret DDP4 to bind MERS-CoV. Site-directed mutagenesis of amino acids variable in ferret DPP4 thus revealed the functional human DPP4 virus binding site. Adenosine deaminase (ADA), a DPP4 binding protein, competed for virus binding, acting as a natural antagonist for MERS-CoV infection. 相似文献
218.