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991.
Jennifer M. Mobberley Christina L. M. Khodadad Jamie S. Foster 《Photosynthesis research》2013,118(1-2):125-140
Thrombolites are unlaminated carbonate deposits formed by the metabolic activities of microbial mats and can serve as potential models for understanding the molecular mechanisms underlying the formation of lithifying communities. To assess the metabolic complexity of these ecosystems, high throughput DNA sequencing of a thrombolitic mat metagenome was coupled with phenotypic microarray analysis. Functional protein analysis of the thrombolite community metagenome delineated several of the major metabolic pathways that influence carbonate mineralization including cyanobacterial photosynthesis, sulfate reduction, sulfide oxidation, and aerobic heterotrophy. Spatial profiling of metabolite utilization within the thrombolite-forming microbial mats suggested that the top 5 mm contained a more metabolically diverse and active community than the deeper within the mat. This study provides evidence that despite the lack of mineral layering within the clotted thrombolite structure there is a vertical gradient of metabolic activity within the thrombolitic mat community. This metagenomic profiling also serves as a foundation for examining the active role individual functional groups of microbes play in coordinating metabolisms that lead to mineralization. 相似文献
992.
P. Foster D. Voltolina J. Beardall 《Journal of experimental marine biology and ecology》1982,62(2):93-115
Physical, chemical, and phytoplankton distributions observed in Liverpool Bay during early and terminal stages in development of the spring bloom in May, 1977, are described. Qualitative and quantitative changes in phytoplankton distributions are interpreted with respect to the contemporaneous chemical and physical hydrography and quantified changes in the hydrography between the periods of observation. In early May, a physical discontinuity demarcated the biological, physical, and most of the chemical characteristics of the offshore and coastal waters. Phytoplankton numbers were greatest in the inshore waters and decreased seaward. The diatom bloom was concentrated in coastal areas where the nutrient status of the waters was least favourable to sustain it. The waters bounded by the front and the coast were composed of a number of physically and chemically distinct water types and each had associated with it a qualitatively distinct phytoplankton population. During the early stage of the spring bloom no abnormal concentrations or accumulations of phytoplankton were associated with the front. By late May areas of diverse phytoplankton density had developed in the inshore waters, major differences in qualitative composition were associated with various density fields and the physical discontinuity, separating offshore and coastal waters, had developed into the site of greatest phytoplankton density. The spatial and temporal changes in the phytoplankton populations are shown to be related to the nutrient status of the waters both within and at the boundaries of the various density fields. 相似文献
993.
Zhang X de Chickera SN Willert C Economopoulos V Noad J Rohani R Wang AY Levings MK Scheid E Foley R Foster PJ Dekaban GA 《Cytotherapy》2011,13(10):1234-1248
BACKGROUND AIMS. The use of dendritic cells (DC) as an adjuvant in cell-based immunotherapeutic cancer vaccines is a growing field of interest. A reliable and non-invasive method to track the fate of autologous DC following their administration to patients is required in order to confirm that clinically sufficient numbers are reaching the lymph node (LN). We demonstrate that an immunocompromised mouse model can be used to conduct translational studies employing cellular magnetic resonance imaging (MRI). Such studies can provide clinically relevant information regarding the migration potential of clinical-grade DC used in cancer immunotherapies. METHODS. Human monocyte-derived dendritic cells (mo-DC) were generated from negatively selected monocytes obtained from either healthy donors or cancer patients. DC were labeled with superparamagnetic iron oxide (SPIO) nanoparticles in order to track them in vivo in a CB17scid mouse model using cellular MRI. SPIO did not have any adverse effects on DC phenotype or function, independent of donor type. Cellular MRI readily detected migration of SPIO-loaded DC in CB17scid mice. No differences in migration were observed between DC obtained from healthy donors and those obtained from donors undergoing autologous stem cell transplant for cancer therapy. CONCLUSIONS. Cellular MRI provided semi-quantitative image data that corresponded with data obtained by digital morphometry, validating cellular MRI's potential to assess DC migration in DC-based cancer immunotherapy clinical trials. 相似文献
994.
David H. Foster 《Biological cybernetics》1972,11(4):223-229
An experiment is described which shows in operation the program set out in Foster (1972a) for the investigation of the invariance transformations of visual recognition. The concern in the present study is with the Lie group of rotations SO(2), and a certain centrally located foveal Landolt ring. By presenting to the visual system this Landolt ring and a rotated image in rapid succession, one attempted to induce a specified rotation-type phi-motion. Two subjects were employed. Both reported the existence of the required type of phi-motion for rotations 0 of the Landolt ring about the visual axis with -2/72/7. By appealing to the basic Proposition 2 of Foster (1972 a), the conclusion is reached that the visual system appears capable of effecting upon a certain centrally located foveal annulus the local 1-parameter group of rotations about the visual axis 0, [–2/7,2/7]. 相似文献
995.
A Critical Review of the Arsenic Uptake Mechanisms and Phytoremediation Potential of Pteris vittata 总被引:1,自引:0,他引:1
Luu Thai Danh Paul Truong Raffaella Mammucari Neil Foster 《International journal of phytoremediation》2014,16(5):429-453
The discovery of the arsenic hyperaccumulator, Pteris vittata (Chinese brake fern), has contributed to the promotion of its application as a means of phytoremediation for arsenic removal from contaminated soils and water. Understanding the mechanisms involved in arsenic tolerance and accumulation of this plant provides valuable tools to improve the phytoremediation efficiency. In this review, the current knowledge about the physiological and molecular mechanisms of arsenic tolerance and accumulation in P. vittata is summarized, and an attempt has been made to clarify some of the unresolved questions related to these mechanisms. In addition, the capacity of P. vittata for remediation of arsenic-contaminated soils is evaluated under field conditions for the first time, and possible solutions to improve the remediation capacity of Pteris vittata are also discussed. 相似文献
996.
Scott RH Whyment AD Foster A Gordon KH Milne BF Jaspars M 《The Journal of membrane biology》2000,176(2):119-131
We have chemically characterized a preparation of halitoxins, (1,3 alkyl-pyridinium salts) isolated from the marine sponge
Callyspongia ridleyi. At concentrations of 50 and 5 μg/ml the halitoxin preparation caused irreversible membrane potential depolarization, decreased
input resistance and inhibited evoked action potentials when applied to cultured dorsal root ganglion neurones. Under whole
cell voltage clamp the halitoxins produced an increase in cation conductance that was attenuated by replacing sodium with
N-methyl-d-glucamine. Fura-2 fluorescence ratiometric calcium imaging was used to directly measure calcium flux into neurones after
exposure to halitoxins. Calcium influx, evoked by the halitoxins, persisted when the neurones were bathed in medium containing
the voltage-activated calcium channel antagonists cadmium and nickel. Experiments on undifferentiated F-11 cells showed little
or no calcium influx in response to depolarizing concentrations of potassium and indicated that halitoxins evoked massive
calcium influx in the absence of voltage-activated calcium channels. The halitoxins also produced transient increases in intracellular
calcium when F-11 cells were bathed in calcium-free medium suggesting that the toxins could release calcium from intracellular
stores. The pore-forming action of the halitoxins was identified when the toxins were applied to artificial lipid bilayers
composed of phosphatidylcholine and cholesterol. Halitoxins evoked channel-like activity in the lipid bilayers, with estimated
unitary conductances of between 145pS and 2280pS, possibly indicating that distinct channels could be produced by the different
components in the preparation of halitoxins.
Received: 23 December 1999/Revised: 3 April 2000 相似文献
997.
Rens W O'Brien PC Grützner F Clarke O Graphodatskaya D Tsend-Ayush E Trifonov VA Skelton H Wallis MC Johnston S Veyrunes F Graves JA Ferguson-Smith MA 《Genome biology》2007,8(11):R243-21
Background
Sex-determining systems have evolved independently in vertebrates. Placental mammals and marsupials have an XY system, birds have a ZW system. Reptiles and amphibians have different systems, including temperature-dependent sex determination, and XY and ZW systems that differ in origin from birds and placental mammals. Monotremes diverged early in mammalian evolution, just after the mammalian clade diverged from the sauropsid clade. Our previous studies showed that male platypus has five X and five Y chromosomes, no SRY, and DMRT1 on an X chromosome. In order to investigate monotreme sex chromosome evolution, we performed a comparative study of platypus and echidna by chromosome painting and comparative gene mapping.Results
Chromosome painting reveals a meiotic chain of nine sex chromosomes in the male echidna and establishes their order in the chain. Two of those differ from those in the platypus, three of the platypus sex chromosomes differ from those of the echidna and the order of several chromosomes is rearranged. Comparative gene mapping shows that, in addition to bird autosome regions, regions of bird Z chromosomes are homologous to regions in four platypus X chromosomes, that is, X1, X2, X3, X5, and in chromosome Y1.Conclusion
Monotreme sex chromosomes are easiest to explain on the hypothesis that autosomes were added sequentially to the translocation chain, with the final additions after platypus and echidna divergence. Genome sequencing and contig anchoring show no homology yet between platypus and therian Xs; thus, monotremes have a unique XY sex chromosome system that shares some homology with the avian Z. 相似文献998.
999.
AMÉLIE VANTAUX SHARON SCHILLEWAERT THOMAS PARMENTIER WIM VAN DEN ENDE JOHAN BILLEN TOM WENSELEERS 《Ecological Entomology》2015,40(5):511-517
1. The aphid Aphis fabae (Scopoli) is facultatively tended by Lasius niger (Linnaeus) ants. Previously, we found that A. fabae colonies can be made up of several clones, and that clones display significant differences in the composition of their honeydew sugars, especially in the amount of the ant attractant sugar melezitose that they produce. 2. These clonal differences could greatly impact the strength of the mutualistic interaction with ants as well as the aphids' fitness. 3. Hence, the aim of this study was to compare the fitness of different A. fabae clones that differed in their melezitose secretion, and whether or not they were tended by ants. 4. Individual fitness indices, colony growth, and alate production of single‐clone aphid colonies were analysed. 5. The results demonstrate that the fitness consequences of ant attendance critically depend on an interaction between levels of melezitose production. In particular, we show that high‐melezitose secreting clones produce fewer alates and hence might have a lower dispersal ability in the presence of ants. 6. Furthermore, these data confirm previous evidence that ant attendance is costly and results in the production of fewer apterae. 相似文献
1000.
I. Heise G. T. Banks S. Wells S. N. Peirson R. G. Foster P. M. Nolan 《Genes, Brain & Behavior》2015,14(2):209-216
Down syndrome is a common disorder associated with intellectual disability in humans. Among a variety of severe health problems, patients with Down syndrome exhibit disrupted sleep and abnormal 24‐h rest/activity patterns. The transchromosomic mouse model of Down syndrome, Tc1, is a trans‐species mouse model for Down syndrome, carrying most of human chromosome 21 in addition to the normal complement of mouse chromosomes and expresses many of the phenotypes characteristic of Down syndrome. To date, however, sleep and circadian rhythms have not been characterized in Tc1 mice. Using both circadian wheel‐running analysis and video‐based sleep scoring, we showed that these mice exhibited fragmented patterns of sleep‐like behaviour during the light phase of a 12:12‐h light/dark (LD) cycle with an extended period of continuous wakefulness at the beginning of the dark phase. Moreover, an acute light pulse during night‐time was less effective in inducing sleep‐like behaviour in Tc1 animals than in wild‐type controls. In wheel‐running analysis, free running in constant light (LL) or constant darkness (DD) showed no changes in the circadian period of Tc1 animals although they did express subtle behavioural differences including a reduction in total distance travelled on the wheel and differences in the acrophase of activity in LD and in DD. Our data confirm that Tc1 mice express sleep‐related phenotypes that are comparable with those seen in Down syndrome patients with moderate disruptions in rest/activity patterns and hyperactive episodes, while circadian period under constant lighting conditions is essentially unaffected. 相似文献