全文获取类型
收费全文 | 344篇 |
免费 | 31篇 |
专业分类
375篇 |
出版年
2021年 | 4篇 |
2020年 | 3篇 |
2018年 | 6篇 |
2017年 | 4篇 |
2016年 | 10篇 |
2015年 | 7篇 |
2014年 | 9篇 |
2013年 | 12篇 |
2012年 | 19篇 |
2011年 | 17篇 |
2010年 | 5篇 |
2009年 | 10篇 |
2008年 | 10篇 |
2007年 | 8篇 |
2006年 | 20篇 |
2005年 | 10篇 |
2004年 | 13篇 |
2003年 | 7篇 |
2002年 | 14篇 |
2001年 | 10篇 |
2000年 | 12篇 |
1999年 | 5篇 |
1998年 | 3篇 |
1996年 | 5篇 |
1995年 | 5篇 |
1994年 | 3篇 |
1992年 | 5篇 |
1991年 | 7篇 |
1990年 | 8篇 |
1989年 | 3篇 |
1988年 | 9篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 6篇 |
1983年 | 3篇 |
1981年 | 6篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 5篇 |
1975年 | 7篇 |
1974年 | 5篇 |
1973年 | 7篇 |
1972年 | 6篇 |
1971年 | 3篇 |
1970年 | 5篇 |
1969年 | 4篇 |
1966年 | 5篇 |
排序方式: 共有375条查询结果,搜索用时 0 毫秒
91.
A pen-reared northern bobwhite and a domestic turkey were infected with a strain of Plasmodium hermani obtained originally from a wild turkey in southern Florida. Blood films from these 2 birds were positive microscopically for 188 and 370 days postinfection (PI), respectively. Culicine mosquitoes (Culex nigripalpus and C. salinarius) were blood fed on the bobwhite and the turkey at different times during the infection and used to transmit the malaria to other bobwhites and turkeys up to days 298 and 473 PI, respectively. It was concluded that in nature, P. hermani could remain in a chronic phase in avian hosts for a year, or longer, allowing survival of the parasite between seasons of mosquito transmission. 相似文献
92.
93.
To determine whether gestation-related changes in responsiveness of the rat uterus to beta-adrenergic agonists are mediated at the level of adenylyl cyclase, we measured myometrial adenylyl cyclase activity and protein quantities during pregnancy and labor. In rat myometrial membranes, basal adenylyl cyclase activity increased from the nonpregnant state to mid (Days 12-14) and then late (Days 18-20) gestation and then decreased intrapartum (Day 22). Stimulated adenylyl cyclase activity, at the level of the beta-adrenergic receptor (isoproterenol, 10(-4) M), the G protein (GTP, 10(-5) M), or the adenylyl cyclase enzyme (MnCl(2), 20 mM), was similarly altered during gestation. Total adenylyl cyclase protein was quantified by [(3)H]forskolin binding assay in myometrial membranes from nonpregnant and pregnant (Day 14, Day 20, Day 21, and intrapartum Day 22) rats. Adenylyl cyclase protein increased progressively from nonpregnant rats to pregnant rats at mid (Day 14) and late (Day 20) gestation, but it decreased abruptly to nonpregnant levels on Day 21, the day before parturition, and remained at similar levels on Day 22 (intrapartum). The gestation-related increase in expression of myometrial adenylyl cyclase protein may facilitate uterine quiescence during pregnancy, and the abrupt decrease of adenylyl cyclase protein on the last day of pregnancy may be a contributing mechanism for the initiation of labor. 相似文献
94.
Asynchronous rat embryo cells expressing Myc were followed in 50 fields by computerized video time lapse (CVTL) for three to four cycles before irradiation (4 Gy) and then for 6-7 days thereafter. Pedigrees were constructed for single cells that had been irradiated in different parts of the cycle, i.e. at different times after they were born. Over 95% of the cell death occurred by postmitotic apoptosis after the cells and their progeny had divided from one to six times. The duration of the process of apoptosis once it was initiated was independent of the phase in which the cell was irradiated. Cell death was defined as cessation of movement, typically 20-60 min after the cell rounded with membrane blebbing, but membrane rupture did not occur until 5 to 40 h later. The times to apoptosis and the number of divisions after irradiation were less for cells irradiated late in the cycle. Cells irradiated in G(1) phase divided one to six times and survived 40-120 h before undergoing apoptosis compared to only one to two times and 5-40 h for cells irradiated in G(2) phase. The only cells that died without dividing after irradiation were irradiated in mid to late S phase. Essentially the same results were observed for a dose of 9.5 Gy, although the progeny died sooner and after fewer divisions than after 4 Gy. Regardless of the phase in which they were irradiated, the cells underwent apoptosis from 2 to 150 h after their last division. Therefore, the postmitotic apoptosis did not occur in a predictable or programmed manner, although apoptosis was associated with lengthening of both the generation time and the duration of mitosis immediately prior to the death of the daughter cells. After the non-clonogenic cells divided and yielded progeny entering the first generation after irradiation with 4 Gy, 60% of the progeny either had micronuclei or were sisters of cells that had micronuclei, compared to none of the progeny of clonogenic cells having micronuclei in generation 1. However, another 20% of the non-clonogenic cells had progeny with micronuclei appearing first in generation 2 or 3. As a result, 80% of the non-clonogenic cells had progeny with micronuclei. Furthermore, cells with micronuclei were more likely to die during the generation in which the micronuclei were observed than cells not having micronuclei. Also, micronuclei were occasionally observed in the progeny from clonogenic cells in later generations at about the same time that lethal sectoring was observed. Thus cell death was associated with formation of micronuclei. Most importantly, cells irradiated in late S or G(2) phase were more radiosensitive than cells irradiated in G(1) phase for both loss of clonogenic survival and the time of death and number of divisions completed after irradiation. Finally, the cumulative percentage of apoptosis scored in whole populations of asynchronous or synchronous populations, without distinguishing between the progeny of individually irradiated cells, underestimates the true amount of apoptosis that occurs in cells that undergo postmitotic apoptosis after irradiation. Scoring cell death in whole populations of cells gives erroneous results since both clonogenic and non-clonogenic cells are dividing as non-clonogenic cells are undergoing apoptosis over a period of many days. 相似文献
95.
Members of the Frizzled family of integral membrane proteins are implicated in many developmental events, including specifying cell fate, orienting cell and planar polarity, and directing cell migration. Frizzleds function as cell surface receptors for secreted Wnt proteins. We report here the isolation of a mutation in cfz-2, a Caenorhabditis elegans Frizzled gene. Mutation of cfz-2 causes defective cell migration, disorganization of head neurons, and can cause ectopic axon outgrowth. Analysis of mosaic animals shows that CFZ-2 functions cell nonautonomously, but does not rule out an autonomous role. CFZ-2 is expressed primarily in the anterior of embryos and in several cells in the head of adults. Our analysis of interactions between CFZ-2 and other Wnt pathways reveals that three Wnts, CWN-1, CWN-2 and EGL-20, and a Frizzled, MOM-5, function redundantly with one another and with CFZ-2 for specific cell migrations. In contrast, CWN-1, CWN-2, EGL-20, CFZ-2, and MOM-5 antagonize one another for other migrations. Therefore, CFZ-2 functions by collaborating with and/or antagonizing other Wnt signaling pathways to regulate specific cell migrations. 相似文献
96.
A gene trap integration provides an early in situ marker for hepatic specification of the foregut endoderm 总被引:1,自引:0,他引:1
Watt AJ Jones EA Ure JM Peddie D Wilson DI Forrester LM 《Mechanisms of development》2001,100(2):1477-215
We report the characterization of a gene trap integration that provides an in situ marker for one of the earliest events in liver development. Expression of the reporter gene is observed at the nine-somite stage in the hepatic field of the foregut endoderm. At 10.5 days post-coitus expression is observed exclusively and at high levels in the majority of cells in the developing liver bud. As development proceeds the proportion of expressing cells decreases with expression in adult liver being restricted to a few sporadic cells. This therefore provides the earliest, most specific in situ marker of the hepatic lineage reported to date and will be useful in the further characterization of the inductive events involved in hepatic specification. Molecular characterization of the gene trap insertion suggests that the expression pattern is the result of alternative promoter use in the ankyrin repeat-containing gene, gtar. 相似文献
97.
98.
Andreas Fichtner David I. Forrester Werner H?rdtle Knut Sturm Goddert von Oheimb 《PloS one》2015,10(3)
The role of competition in tree communities is increasingly well understood, while little is known about the patterns and mechanisms of the interplay between above- and belowground competition in tree communities. This knowledge, however, is crucial for a better understanding of community dynamics and developing adaptive near-natural management strategies. We assessed neighbourhood interactions in an unmanaged old-growth European beech (Fagus sylvatica) forest by quantifying variation in the intensity of above- (shading) and belowground competition (crowding) among dominant and co-dominant canopy beech trees during tree maturation. Shading had on average a much larger impact on radial growth than crowding and the sensitivity to changes in competitive conditions was lowest for crowding effects. We found that each mode of competition reduced the effect of the other. Increasing crowding reduced the negative effect of shading, and at high levels of shading, crowding actually had a facilitative effect and increased growth. Our study demonstrates that complementarity in above- and belowground processes enable F. sylvatica to alter resource acquisition strategies, thus optimising tree radial growth. As a result, competition seemed to become less important in stands with a high growing stock and tree communities with a long continuity of anthropogenic undisturbed population dynamics. We suggest that growth rates do not exclusively depend on the density of potential competitors at the intraspecific level, but on the conspecific aggregation of large-diameter trees and their functional role for regulating biotic filtering processes. This finding highlights the potential importance of the rarely examined relationship between the spatial aggregation pattern of large-diameter trees and the outcome of neighbourhood interactions, which may be central to community dynamics and the related forest ecosystem services. 相似文献
99.
Simon V. Fowler Robert Barreto Sarah Dodd Davi M. Macedo Quentin Paynter José H. Pedrosa-Macedo Olinto L. Pereira Paul Peterson Lindsay Smith Nick Waipara Chris J. Winks Guy Forrester 《Biological Control》2013,64(3):323-329
In the native range of Tradescantia fluminensis in SE Brazil surveys revealed a natural enemy biota attacking the plant that was rich in potential biocontrol agents for New Zealand (NZ), including nine fungi and 10 herbivorous insect species. Similar surveys in NZ, where T. fluminensis is an invasive exotic weed, revealed no specialist insect herbivores or pathogens. The Brazilian insect herbivores included leafmining, stemboring and gall-forming feeding guilds that were absent in NZ. Mean foliar damage levels per site on T. fluminensis were 7.8× higher for folivores in Brazil cf. NZ, and 21.2× higher for pathogens. The presence of rust pustules, or ‘brown lesions’, on leaves in Brazil was negatively associated with damage by folivores, perhaps indicating an antagonistic interaction. In contrast, damage by the white smut fungus, Kordyana sp., was not negatively associated with folivore damage. Mean dry biomass of T. fluminensis was significantly lower in Brazil (164 g m?2) cf. NZ (455 g m?2). In NZ, 85% of sites had biomass measures >200 g m?2 (the previously determined threshold above which native forest regeneration fails). In Brazil, only 27% of sites had biomass measures >200 g m?2. Among the insect herbivores, three chrysomelid beetles, Neolema ogloblini, Neolema abbreviata and Lema basicostata were prioritised as potential biocontrol agents. Their larvae cause potentially complementary damage to leaves, shoot-tips and mature stems, respectively. Several pathogens, including a rust, were rejected before we selected the Kordyana species. Host range testing of all four agents showed sufficient host-specificity for consideration for release in NZ. Neolema ogloblini and L. basicostata were field-released in NZ in 2011 and 2012, with the field-release of N. abbreviata due late 2012. An application to release Kordyana sp. in NZ has been made. 相似文献
100.
Herman Pontzer Mary H. Brown Brian M. Wood David A. Raichlen Audax.Z.P. Mabulla Jacob A. Harris Holly Dunsworth Brian Hare Kara Walker Amy Luke Lara R. Dugas Dale Schoeller Jacob Plange-Rhule Pascal Bovet Terrence E. Forrester Melissa Emery Thompson Robert W. Shumaker Jessica M. Rothman Stephen R. Ross 《Current biology : CB》2021,31(8):1804-1810.e5