全文获取类型
收费全文 | 461篇 |
免费 | 47篇 |
出版年
2021年 | 5篇 |
2020年 | 5篇 |
2019年 | 5篇 |
2018年 | 7篇 |
2017年 | 7篇 |
2016年 | 7篇 |
2015年 | 13篇 |
2014年 | 14篇 |
2013年 | 20篇 |
2012年 | 17篇 |
2011年 | 14篇 |
2010年 | 11篇 |
2009年 | 9篇 |
2008年 | 16篇 |
2007年 | 9篇 |
2006年 | 15篇 |
2005年 | 13篇 |
2004年 | 6篇 |
2003年 | 6篇 |
2002年 | 6篇 |
2001年 | 8篇 |
2000年 | 10篇 |
1999年 | 10篇 |
1998年 | 15篇 |
1997年 | 7篇 |
1996年 | 9篇 |
1993年 | 7篇 |
1992年 | 10篇 |
1991年 | 10篇 |
1990年 | 20篇 |
1989年 | 13篇 |
1988年 | 14篇 |
1987年 | 15篇 |
1986年 | 6篇 |
1985年 | 12篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1981年 | 5篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1976年 | 7篇 |
1975年 | 4篇 |
1974年 | 5篇 |
1973年 | 4篇 |
1972年 | 12篇 |
1971年 | 7篇 |
1970年 | 10篇 |
1969年 | 8篇 |
1968年 | 7篇 |
排序方式: 共有508条查询结果,搜索用时 31 毫秒
41.
Survival of embryos exposed to several concentrations of uterine proteins and changes in tubal morphology in rabbits given low preovulatory doses of progesterone (P4) that had previously not affected ovulation or fertilization, but caused severe embryo mortality, were studied. In experiment 1, 332 morulae were cultured for 24 h in a control medium containing < 0.5 to > 3.0 mg x mL(-1) of Day 3 uterine fluid proteins. There was no difference in blastocyst development nor implantation to Day 12 following transfer of the blastocysts to recipients, except fewer implants developed in the BSA control. In experiment 2 the oviducts and uteri of control and P4-treated does were examined by SEM for 8 days following ovulation. Secretory cells in the oviducts and to a lesser extent in the uteri were stimulated by P4 treatment for 3 to 4 days after ovulation. Morphology of ciliated cells was unaffected. The subtle changes did not fully account for P4-induced embryo mortality in vivo. 相似文献
42.
43.
What can bioinformatics do for parasitology research? 总被引:11,自引:0,他引:11
44.
Ben C Collins Ludovic CJ Gillet Lorenz C Blum Lin‐Yang Cheng Olga Vitek Jeppe Mouritsen Genevieve Lachance Tim D Spector Emmanouil T Dermitzakis Ruedi Aebersold 《Molecular systems biology》2015,11(2)
The degree and the origins of quantitative variability of most human plasma proteins are largely unknown. Because the twin study design provides a natural opportunity to estimate the relative contribution of heritability and environment to different traits in human population, we applied here the highly accurate and reproducible SWATH mass spectrometry technique to quantify 1,904 peptides defining 342 unique plasma proteins in 232 plasma samples collected longitudinally from pairs of monozygotic and dizygotic twins at intervals of 2–7 years, and proportioned the observed total quantitative variability to its root causes, genes, and environmental and longitudinal factors. The data indicate that different proteins show vastly different patterns of abundance variability among humans and that genetic control and longitudinal variation affect protein levels and biological processes to different degrees. The data further strongly suggest that the plasma concentrations of clinical biomarkers need to be calibrated against genetic and temporal factors. Moreover, we identified 13 cis‐SNPs significantly influencing the level of specific plasma proteins. These results therefore have immediate implications for the effective design of blood‐based biomarker studies. 相似文献
45.
Con Dogovski Stanley C. Xie Gaetan Burgio Jess Bridgford Sachel Mok James M. McCaw Kesinee Chotivanich Shannon Kenny Nina Gn?dig Judith Straimer Zbynek Bozdech David A. Fidock Julie A. Simpson Arjen M. Dondorp Simon Foote Nectarios Klonis Leann Tilley 《PLoS biology》2015,13(4)
Successful control of falciparum malaria depends greatly on treatment with artemisinin combination therapies. Thus, reports that resistance to artemisinins (ARTs) has emerged, and that the prevalence of this resistance is increasing, are alarming. ART resistance has recently been linked to mutations in the K13 propeller protein. We undertook a detailed kinetic analysis of the drug responses of K13 wild-type and mutant isolates of Plasmodium falciparum sourced from a region in Cambodia (Pailin). We demonstrate that ART treatment induces growth retardation and an accumulation of ubiquitinated proteins, indicative of a cellular stress response that engages the ubiquitin/proteasome system. We show that resistant parasites exhibit lower levels of ubiquitinated proteins and delayed onset of cell death, indicating an enhanced cell stress response. We found that the stress response can be targeted by inhibiting the proteasome. Accordingly, clinically used proteasome inhibitors strongly synergize ART activity against both sensitive and resistant parasites, including isogenic lines expressing mutant or wild-type K13. Synergy is also observed against Plasmodium berghei in vivo. We developed a detailed model of parasite responses that enables us to infer, for the first time, in vivo parasite clearance profiles from in vitro assessments of ART sensitivity. We provide evidence that the clinical marker of resistance (delayed parasite clearance) is an indirect measure of drug efficacy because of the persistence of unviable parasites with unchanged morphology in the circulation, and we suggest alternative approaches for the direct measurement of viability. Our model predicts that extending current three-day ART treatment courses to four days, or splitting the doses, will efficiently clear resistant parasite infections. This work provides a rationale for improving the detection of ART resistance in the field and for treatment strategies that can be employed in areas with ART resistance. 相似文献
46.
47.
Masa-aki Higuchi Dan D. Topiol Bilal Ahmed Hokuto Morita Samuel Carbunaru Christopher W. Hess Dawn Bowers Herbert E. Ward Lisa R. Warren Meredith M. DeFranco Michelle S. Troche Shankar J. Kulkarni Erin Hastings Kelly D. Foote Michael S. Okun Daniel Martinez-Ramirez 《PloS one》2015,10(12)
Objective
To investigate the relationship of our interdisciplinary screening process on post-operative unintended hospitalizations and quality of life.Background
There are currently no standardized criteria for selection of appropriate Deep Brain Stimulation candidates and little hard data exists to support the use of any singular method.Methods
An Essential Tremor cohort was selected from our institutional Deep Brain Stimulation database. The interdisciplinary model utilized seven specialties who pre-operatively screened all potential Deep Brain Stimulation candidates. Concerns for surgery raised by each specialty were documented and classified as none, minor, or major. Charts were reviewed to identify unintended hospitalizations and quality of life measurements at 1 year post-surgery.Results
Eighty-six percent (44/51) of the potential screened candidates were approved for Deep Brain Stimulation. Eight (18%) patients had an unintended hospitalization during the follow-up period. Patients with minor or major concerns raised by any specialty service had significantly more unintended hospitalizations when compared to patients without concerns (75% vs. 25%, p < 0.005). The rate of hospitalization revealed a direct relationship to the “level of concern”; ranging from 100% if major concerns, 42% if minor concerns, and 7% if no concerns raised, p = 0.001. Quality of life scores significantly worsened in patients with unintended hospitalizations at 6 (p = 0.046) and 12 months (p = 0.027) when compared to baseline scores. No significant differences in tremor scores between unintended and non-unintended hospitalizations were observed.Conclusions
The number and level of concerns raised during interdisciplinary Deep Brain Stimulation screenings were significantly related to unintended hospitalizations and to a reduced quality of life. The interdisciplinary evaluation may help to stratify risk for these complications. However, data should be interpreted with caution due to the limitations of our study. Further prospective comparative and larger studies are required to confirm our results. 相似文献48.
A.G. Fowler A.M. Foote M.F. Moody P. Vachette S.W. Prrovencher A. Gabriel J. Bordas M.H.J. Koch 《Journal of biochemical and biophysical methods》1983,7(4):317-329
We have constructed an experimental system, under remote control, for stopped-flow X-ray scattering using synchrotron radiation. It has been used, in conjunction with an annular detector and its associated electronics, to obtain good scattering curves, with time-slices as short as 200 ms, in a new study of the dissociation of the enzyme complex aspartate transcarbamylase. The data have been analysed by new statistical methods, and they agree well with the results from parallel chemical quench experiments. For studying dissociation reactions, stopped-flow X-ray scattering is a quite practical method, which need not use very much more material than conventional stopped-flow experiments. 相似文献
49.
Thomson R Quinn S McKay J Silver J Bahlo M FitzGerald L Foote S Dickinson J Stankovich J 《Human genetics》2007,121(3-4):459-468
Dense sets of hundreds of thousands of markers have been developed for genome-wide association studies. These marker sets
are also beneficial for linkage analysis of large, deep pedigrees containing distantly related cases. It is impossible to
analyse jointly all genotypes in large pedigrees using the Lander–Green Algorithm, however, as marker density increases it
becomes less crucial to analyse all individuals’ genotypes simultaneously. In this report, an approximate multipoint non-parametric
technique is described, where large pedigrees are split into many small pedigrees, each containing just two cases. This technique
is demonstrated, using phased data from the International Hapmap Project to simulate sets of 10,000, 50,000 and 250,000 markers,
showing that it becomes increasingly accurate as more markers are genotyped. This method allows routine linkage analysis of
large families with dense marker sets and represents a more easily applied alternative to Monte Carlo Markov Chain methods. 相似文献
50.